Universal toxin-based selection for precise genome engineering in human cells
Genome engineering in cell lines or human stem cells often has poor efficiency, limiting the development of research and therapeutic applications. Here, the authors use a toxin-based selection system for precise bi-allelic engineering in cells and in vivo.
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2021-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20810-z |
| id |
doaj-5902061ff2c8407598c33e5d4d157a46 |
|---|---|
| record_format |
Article |
| spelling |
doaj-5902061ff2c8407598c33e5d4d157a462021-01-24T12:12:14ZengNature Publishing GroupNature Communications2041-17232021-01-0112111410.1038/s41467-020-20810-zUniversal toxin-based selection for precise genome engineering in human cellsSongyuan Li0Nina Akrap1Silvia Cerboni2Michelle J. Porritt3Sandra Wimberger4Anders Lundin5Carl Möller6Mike Firth7Euan Gordon8Bojana Lazovic9Aleksandra Sieńska10Luna Simona Pane11Matthew A. Coelho12Giovanni Ciotta13Giovanni Pellegrini14Marcella Sini15Xiufeng Xu16Suman Mitra17Mohammad Bohlooly-Y18Benjamin J. M. Taylor19Grzegorz Sienski20Marcello Maresca21Translational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Science and Experimental Medicine, Respiratory & Immunology, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaR&D Data Infrastructure & Tools, AstraZenecaDiscovery Biology SWE, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaWellcome Sanger InstituteDiscovery Biology UK, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaCVRM pathology, Clinical Pharmacology & Safety Sciences, BioPharmaceuticals R&D, AstraZenecaCVRM pathology, Clinical Pharmacology & Safety Sciences, BioPharmaceuticals R&D, AstraZenecaDepartment of Biosciences and Nutrition, Karolinska InstituteInserm UMR1277 CNRS UMR9020 – CANTHER, Institut pour la Recherche sur le Cancer de LilleTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaDiscovery Biology UK, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaTranslational Genomics, Discovery Sciences, BioPharmaceuticals R&D, AstraZenecaGenome engineering in cell lines or human stem cells often has poor efficiency, limiting the development of research and therapeutic applications. Here, the authors use a toxin-based selection system for precise bi-allelic engineering in cells and in vivo.https://doi.org/10.1038/s41467-020-20810-z |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Songyuan Li Nina Akrap Silvia Cerboni Michelle J. Porritt Sandra Wimberger Anders Lundin Carl Möller Mike Firth Euan Gordon Bojana Lazovic Aleksandra Sieńska Luna Simona Pane Matthew A. Coelho Giovanni Ciotta Giovanni Pellegrini Marcella Sini Xiufeng Xu Suman Mitra Mohammad Bohlooly-Y Benjamin J. M. Taylor Grzegorz Sienski Marcello Maresca |
| spellingShingle |
Songyuan Li Nina Akrap Silvia Cerboni Michelle J. Porritt Sandra Wimberger Anders Lundin Carl Möller Mike Firth Euan Gordon Bojana Lazovic Aleksandra Sieńska Luna Simona Pane Matthew A. Coelho Giovanni Ciotta Giovanni Pellegrini Marcella Sini Xiufeng Xu Suman Mitra Mohammad Bohlooly-Y Benjamin J. M. Taylor Grzegorz Sienski Marcello Maresca Universal toxin-based selection for precise genome engineering in human cells Nature Communications |
| author_facet |
Songyuan Li Nina Akrap Silvia Cerboni Michelle J. Porritt Sandra Wimberger Anders Lundin Carl Möller Mike Firth Euan Gordon Bojana Lazovic Aleksandra Sieńska Luna Simona Pane Matthew A. Coelho Giovanni Ciotta Giovanni Pellegrini Marcella Sini Xiufeng Xu Suman Mitra Mohammad Bohlooly-Y Benjamin J. M. Taylor Grzegorz Sienski Marcello Maresca |
| author_sort |
Songyuan Li |
| title |
Universal toxin-based selection for precise genome engineering in human cells |
| title_short |
Universal toxin-based selection for precise genome engineering in human cells |
| title_full |
Universal toxin-based selection for precise genome engineering in human cells |
| title_fullStr |
Universal toxin-based selection for precise genome engineering in human cells |
| title_full_unstemmed |
Universal toxin-based selection for precise genome engineering in human cells |
| title_sort |
universal toxin-based selection for precise genome engineering in human cells |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-01-01 |
| description |
Genome engineering in cell lines or human stem cells often has poor efficiency, limiting the development of research and therapeutic applications. Here, the authors use a toxin-based selection system for precise bi-allelic engineering in cells and in vivo. |
| url |
https://doi.org/10.1038/s41467-020-20810-z |
| work_keys_str_mv |
AT songyuanli universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT ninaakrap universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT silviacerboni universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT michellejporritt universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT sandrawimberger universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT anderslundin universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT carlmoller universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT mikefirth universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT euangordon universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT bojanalazovic universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT aleksandrasienska universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT lunasimonapane universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT matthewacoelho universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT giovanniciotta universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT giovannipellegrini universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT marcellasini universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT xiufengxu universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT sumanmitra universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT mohammadbohloolyy universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT benjaminjmtaylor universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT grzegorzsienski universaltoxinbasedselectionforprecisegenomeengineeringinhumancells AT marcellomaresca universaltoxinbasedselectionforprecisegenomeengineeringinhumancells |
| _version_ |
1724326187883298816 |