Dexmedetomidine affects the malignant biological behavior of glioma cells via miR-760/HDGF
Objective: To investigate the effect of dexmedetomidine on the malignant biological behavior of glioma cells and its mechanism. Methods: Human glioma cell line A172 was cultured in vitro and treated with different concentrations (50, 100, 200, 400, 800 mg / L) of dexmedetomidine. The effect of de...
Main Author: | |
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Format: | Article |
Language: | English |
Published: |
Editorial Board of Journal of Hainan Medical University
2020-04-01
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Series: | Journal of Hainan Medical University |
Subjects: | |
Online Access: | http://www.hnykdxxb.com/PDF/202020/04.pdf |
Summary: | Objective: To investigate the effect of dexmedetomidine on the malignant biological
behavior of glioma cells and its mechanism. Methods: Human glioma cell line A172 was
cultured in vitro and treated with different concentrations (50, 100, 200, 400, 800 mg / L)
of dexmedetomidine. The effect of dexmedetomidine on the proliferation of A172 cells
was detected by MTT. Flow cytometry was used to detect the effect of dexmedetomidine
on the apoptosis of A172 cells. Transwell chamber assay was used to detect the effect of
dexmedetomidine on the migration and invasion of A172 cells. The effects of dexmedetomidine
on the expression of miR-760 and HDGF were detected by real-time quantitative PCR (qRTPCR)
and Western blot. Dual luciferase reporter assays were used to examine the effect of
miR-760 overexpression on wild-type and mutant HDGF luciferase activities. miR-760 mimics
and miR-con were transfected into A172 cells to detect changes in cell proliferation, apoptosis,
migration and invasion. Western blot was used to detect the expression of MMP-2, MMP-9,
Cleaved caspase-3 and Cleaved PARP protein. Results: Dexmedetomidine significantly
reduced the survival rate of glioma cells (P<0.05), decreased the number of migrated cells
and the number of invasive cells (P<0.05), and inhibited the expression of HDGF, MMP-2
and MMP-9 (P<0.05). However, it could increase the apoptosis rate (P<0.05) and promote
the expression of miR-760, Cleaved caspase-3 and Cleaved PARP (P<0.05). The effect
was obvious when the concentration reaches 800 mg / L. Luciferase reporter gene results
confirmed that miR-760 can inhibit the luciferase activity of the 3'-UTR region of HDGF
(P<0.05). After transfection of miR-760 mimics, cell viability was significantly lower than
that of miR-con group (P<0.05), and the number of migrated cells and invasive cells were
significantly decreased (P<0.05), and the expression levels of MMP-2 and MMP-9 were
significantly decreased (P<0.05), but the apoptosis rate was significantly increased (P<0.05),
and the expression levels of Cleaved caspase-3 and Cleaved PARP were significantly increased
(P<0.05). Interference with the expression of miR-760 reversed the effect of dexmedetomidine
on the biological behavior of glioma cells. Conclusion: Dexmedetomidine attenuates the
proliferation, migration and invasion of glioma cells through miR-760/HDGF and promotes
apoptosis. |
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ISSN: | 1007-1237 1007-1237 |