Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of...
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doaj-5922b66ae1054d59a7f15c7de4223e912020-11-25T02:23:52ZengMDPI AGPolymers2073-43602020-04-011285385310.3390/polym12040853Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone RegenerationDewi Sartika0Chih-Hsin Wang1Ding-Han Wang2Juin-Hong Cherng3Shu-Jen Chang4Gang-Yi Fan5Yi-Wen Wang6Chian-Her Lee7Po-Da Hong8Chih-Chien Wang9Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, Taipei 114, TaiwanDepartment of Plastic and Reconstructive Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, TaiwanDepartment of Dentistry, School of Dentistry, National Yang-Ming University, Taipei 112, TaiwanDepartment and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 114, TaiwanLaboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, Taipei 114, TaiwanLaboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, Taipei 114, TaiwanDepartment and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 114, TaiwanDepartment of Orthopedics, Taipei Medical University Hospital, Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Materials Sciences and Engineering, National Taiwan University of Science and Technology, Taipei 106, TaiwanDepartment of Orthopedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, TaiwanRecently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (<i>BMP-2</i>, <i>COL1a1</i>, and <i>OCN</i>) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.https://www.mdpi.com/2073-4360/12/4/853silk fibroinscaffoldadipose stem cellsbone regenerationcalvarial defectsbone tissue engineering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dewi Sartika Chih-Hsin Wang Ding-Han Wang Juin-Hong Cherng Shu-Jen Chang Gang-Yi Fan Yi-Wen Wang Chian-Her Lee Po-Da Hong Chih-Chien Wang |
spellingShingle |
Dewi Sartika Chih-Hsin Wang Ding-Han Wang Juin-Hong Cherng Shu-Jen Chang Gang-Yi Fan Yi-Wen Wang Chian-Her Lee Po-Da Hong Chih-Chien Wang Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration Polymers silk fibroin scaffold adipose stem cells bone regeneration calvarial defects bone tissue engineering |
author_facet |
Dewi Sartika Chih-Hsin Wang Ding-Han Wang Juin-Hong Cherng Shu-Jen Chang Gang-Yi Fan Yi-Wen Wang Chian-Her Lee Po-Da Hong Chih-Chien Wang |
author_sort |
Dewi Sartika |
title |
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration |
title_short |
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration |
title_full |
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration |
title_fullStr |
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration |
title_full_unstemmed |
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration |
title_sort |
human adipose-derived mesenchymal stem cells-incorporated silk fibroin as a potential bio-scaffold in guiding bone regeneration |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2020-04-01 |
description |
Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (<i>BMP-2</i>, <i>COL1a1</i>, and <i>OCN</i>) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications. |
topic |
silk fibroin scaffold adipose stem cells bone regeneration calvarial defects bone tissue engineering |
url |
https://www.mdpi.com/2073-4360/12/4/853 |
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