Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.

Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, avai...

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Main Authors: Mohammed A K Mahdy, Hesham M Al-Mekhlafi, Abdulsalam M Al-Mekhlafi, Yvonne A L Lim, Naemah O M Bin Shuaib, Ahmed A Azazy, Rohela Mahmud
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-09-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2942841?pdf=render
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spelling doaj-592373020adb49c5bf918878a3856ec52020-11-25T01:22:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-09-015910.1371/journal.pone.0012879Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.Mohammed A K MahdyHesham M Al-MekhlafiAbdulsalam M Al-MekhlafiYvonne A L LimNaemah O M Bin ShuaibAhmed A AzazyRohela MahmudCutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited.This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The sequences from Leishmania isolates were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types.The predominance of the anthroponotic species (i.e. L. tropica) indicates the probability of anthroponotic transmission of cutaneous leishmaniasis in Yemen. These findings will help public health authorities to build an effective control strategy taking into consideration person-to-person transmission as the main dynamic of transmission of CL.http://europepmc.org/articles/PMC2942841?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Mohammed A K Mahdy
Hesham M Al-Mekhlafi
Abdulsalam M Al-Mekhlafi
Yvonne A L Lim
Naemah O M Bin Shuaib
Ahmed A Azazy
Rohela Mahmud
spellingShingle Mohammed A K Mahdy
Hesham M Al-Mekhlafi
Abdulsalam M Al-Mekhlafi
Yvonne A L Lim
Naemah O M Bin Shuaib
Ahmed A Azazy
Rohela Mahmud
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
PLoS ONE
author_facet Mohammed A K Mahdy
Hesham M Al-Mekhlafi
Abdulsalam M Al-Mekhlafi
Yvonne A L Lim
Naemah O M Bin Shuaib
Ahmed A Azazy
Rohela Mahmud
author_sort Mohammed A K Mahdy
title Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
title_short Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
title_full Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
title_fullStr Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
title_full_unstemmed Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
title_sort molecular characterization of leishmania species isolated from cutaneous leishmaniasis in yemen.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2010-09-01
description Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited.This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The sequences from Leishmania isolates were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types.The predominance of the anthroponotic species (i.e. L. tropica) indicates the probability of anthroponotic transmission of cutaneous leishmaniasis in Yemen. These findings will help public health authorities to build an effective control strategy taking into consideration person-to-person transmission as the main dynamic of transmission of CL.
url http://europepmc.org/articles/PMC2942841?pdf=render
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