Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.
Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, avai...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-09-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2942841?pdf=render |
id |
doaj-592373020adb49c5bf918878a3856ec5 |
---|---|
record_format |
Article |
spelling |
doaj-592373020adb49c5bf918878a3856ec52020-11-25T01:22:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-09-015910.1371/journal.pone.0012879Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen.Mohammed A K MahdyHesham M Al-MekhlafiAbdulsalam M Al-MekhlafiYvonne A L LimNaemah O M Bin ShuaibAhmed A AzazyRohela MahmudCutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited.This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The sequences from Leishmania isolates were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types.The predominance of the anthroponotic species (i.e. L. tropica) indicates the probability of anthroponotic transmission of cutaneous leishmaniasis in Yemen. These findings will help public health authorities to build an effective control strategy taking into consideration person-to-person transmission as the main dynamic of transmission of CL.http://europepmc.org/articles/PMC2942841?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammed A K Mahdy Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Yvonne A L Lim Naemah O M Bin Shuaib Ahmed A Azazy Rohela Mahmud |
spellingShingle |
Mohammed A K Mahdy Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Yvonne A L Lim Naemah O M Bin Shuaib Ahmed A Azazy Rohela Mahmud Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. PLoS ONE |
author_facet |
Mohammed A K Mahdy Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Yvonne A L Lim Naemah O M Bin Shuaib Ahmed A Azazy Rohela Mahmud |
author_sort |
Mohammed A K Mahdy |
title |
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. |
title_short |
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. |
title_full |
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. |
title_fullStr |
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. |
title_full_unstemmed |
Molecular characterization of Leishmania species isolated from cutaneous leishmaniasis in Yemen. |
title_sort |
molecular characterization of leishmania species isolated from cutaneous leishmaniasis in yemen. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-09-01 |
description |
Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited.This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The sequences from Leishmania isolates were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. The trees identified Leishmania tropica from 16 isolates which were represented by two sequence types.The predominance of the anthroponotic species (i.e. L. tropica) indicates the probability of anthroponotic transmission of cutaneous leishmaniasis in Yemen. These findings will help public health authorities to build an effective control strategy taking into consideration person-to-person transmission as the main dynamic of transmission of CL. |
url |
http://europepmc.org/articles/PMC2942841?pdf=render |
work_keys_str_mv |
AT mohammedakmahdy molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT heshammalmekhlafi molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT abdulsalammalmekhlafi molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT yvonneallim molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT naemahombinshuaib molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT ahmedaazazy molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen AT rohelamahmud molecularcharacterizationofleishmaniaspeciesisolatedfromcutaneousleishmaniasisinyemen |
_version_ |
1725127684016570368 |