Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose
The endocannabinoid system (ECS) is composed of a group of Gi-coupled protein receptors and enzymes, producing and degrading the endocannabinoids, 2-arachidonoylglycerol (2-AG) and N-arachidonoyl-ethanolamine (AEA). Endocannabinoid-mediated signaling modulates brain functions, such as pain, mood, me...
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Frontiers Media S.A.
2019-09-01
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Series: | Frontiers in Endocrinology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00647/full |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alejandra Palma-Chavez Alejandra Palma-Chavez Macarena Konar-Nié Patricio Órdenes Felipe Maurelia Roberto Elizondo-Vega Karina Oyarce Sergio López Joaquin Rojas Ximena Steinberg María A. García-Robles María A. García-Robles Fernando J. Sepúlveda Fernando J. Sepúlveda |
spellingShingle |
Alejandra Palma-Chavez Alejandra Palma-Chavez Macarena Konar-Nié Patricio Órdenes Felipe Maurelia Roberto Elizondo-Vega Karina Oyarce Sergio López Joaquin Rojas Ximena Steinberg María A. García-Robles María A. García-Robles Fernando J. Sepúlveda Fernando J. Sepúlveda Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose Frontiers in Endocrinology feeding behavior endocannabinoid system hypothalamus DAGLα Tanycytes |
author_facet |
Alejandra Palma-Chavez Alejandra Palma-Chavez Macarena Konar-Nié Patricio Órdenes Felipe Maurelia Roberto Elizondo-Vega Karina Oyarce Sergio López Joaquin Rojas Ximena Steinberg María A. García-Robles María A. García-Robles Fernando J. Sepúlveda Fernando J. Sepúlveda |
author_sort |
Alejandra Palma-Chavez |
title |
Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose |
title_short |
Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose |
title_full |
Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose |
title_fullStr |
Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose |
title_full_unstemmed |
Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose |
title_sort |
glucose increase daglα levels in tanycytes and its inhibition alters orexigenic and anorexigenic neuropeptides expression in response to glucose |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Endocrinology |
issn |
1664-2392 |
publishDate |
2019-09-01 |
description |
The endocannabinoid system (ECS) is composed of a group of Gi-coupled protein receptors and enzymes, producing and degrading the endocannabinoids, 2-arachidonoylglycerol (2-AG) and N-arachidonoyl-ethanolamine (AEA). Endocannabinoid-mediated signaling modulates brain functions, such as pain, mood, memory, and feeding behavior. The activation of the ECS is associated with overeating and obesity; however, the expression of components of this system has been only partially studied in the hypothalamus, a critical region implicated in feeding behavior. Within this brain region, anorexigenic, and orexigenic neurons of the arcuate nucleus (ARC) are in close contact with tanycytes, glial radial-like cells that line the lateral walls and floor of the third ventricle (3V). The specific function of tanycytes and the effects of metabolic signals generated by them on adjacent neurons is starting to be elucidated. We have proposed that the ECS within tanycytes modulates ARC neurons, thus modifying food intake. Here, we evaluated the expression and the loss of function of the 2-AG-producing enzyme, diacylglycerol lipase-alpha (DAGLα). Using Western blot and immunohistochemistry analyses in basal hypothalamus sections of adult rats under several glycemic conditions, we confirm that DAGLα is strongly expressed at the basal hypothalamus in glial and neuronal cells, increasing further in response to greater extracellular glucose levels. Using a DAGLα-inhibiting adenovirus (shRNA), suppression of DAGLα expression in tanycytes altered the usual response to intracerebroventricular glucose in terms of neuropeptides produced by neurons of the ARC. Thus, these results strongly suggest that the tanycytes could generate 2-AG, which modulates the function of anorexigenic and orexigenic neurons. |
topic |
feeding behavior endocannabinoid system hypothalamus DAGLα Tanycytes |
url |
https://www.frontiersin.org/article/10.3389/fendo.2019.00647/full |
work_keys_str_mv |
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doaj-592656f21fca4de3b57d1aef07b7bb6b2020-11-25T01:22:58ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922019-09-011010.3389/fendo.2019.00647457243Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to GlucoseAlejandra Palma-Chavez0Alejandra Palma-Chavez1Macarena Konar-Nié2Patricio Órdenes3Felipe Maurelia4Roberto Elizondo-Vega5Karina Oyarce6Sergio López7Joaquin Rojas8Ximena Steinberg9María A. García-Robles10María A. García-Robles11Fernando J. Sepúlveda12Fernando J. Sepúlveda13Laboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileLaboratorio de Bioquímica y Biología Celular, Departamento de Bioquímica y Biología Molecular, Universidad de Concepción, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileLaboratorio de Bioquímica y Biología Celular, Departamento de Bioquímica y Biología Molecular, Universidad de Concepción, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileFacultad de Medicina y Ciencia, Universidad San Sebastián, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileCentro de Estudios Avanzados para la Vida (CREAV), Universidad de Concepción, Concepción, ChileLaboratorio de Bioquímica y Biología Celular, Departamento de Bioquímica y Biología Molecular, Universidad de Concepción, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileCentro de Estudios Avanzados para la Vida (CREAV), Universidad de Concepción, Concepción, ChileLaboratorio de Biología Celular, Departamento de Biología Celular, Universidad de Concepcion, Concepción, ChileLaboratorio de Bioquímica y Biología Celular, Departamento de Bioquímica y Biología Molecular, Universidad de Concepción, Concepción, ChileThe endocannabinoid system (ECS) is composed of a group of Gi-coupled protein receptors and enzymes, producing and degrading the endocannabinoids, 2-arachidonoylglycerol (2-AG) and N-arachidonoyl-ethanolamine (AEA). Endocannabinoid-mediated signaling modulates brain functions, such as pain, mood, memory, and feeding behavior. The activation of the ECS is associated with overeating and obesity; however, the expression of components of this system has been only partially studied in the hypothalamus, a critical region implicated in feeding behavior. Within this brain region, anorexigenic, and orexigenic neurons of the arcuate nucleus (ARC) are in close contact with tanycytes, glial radial-like cells that line the lateral walls and floor of the third ventricle (3V). The specific function of tanycytes and the effects of metabolic signals generated by them on adjacent neurons is starting to be elucidated. We have proposed that the ECS within tanycytes modulates ARC neurons, thus modifying food intake. Here, we evaluated the expression and the loss of function of the 2-AG-producing enzyme, diacylglycerol lipase-alpha (DAGLα). Using Western blot and immunohistochemistry analyses in basal hypothalamus sections of adult rats under several glycemic conditions, we confirm that DAGLα is strongly expressed at the basal hypothalamus in glial and neuronal cells, increasing further in response to greater extracellular glucose levels. Using a DAGLα-inhibiting adenovirus (shRNA), suppression of DAGLα expression in tanycytes altered the usual response to intracerebroventricular glucose in terms of neuropeptides produced by neurons of the ARC. Thus, these results strongly suggest that the tanycytes could generate 2-AG, which modulates the function of anorexigenic and orexigenic neurons.https://www.frontiersin.org/article/10.3389/fendo.2019.00647/fullfeeding behaviorendocannabinoid systemhypothalamusDAGLαTanycytes |