Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02.
Plasmodium falciparum Apical Membrane Antigen 1 (PfAMA1) is a candidate vaccine antigen expressed by merozoites and sporozoites. It plays a key role in red blood cell and hepatocyte invasion that can be blocked by antibodies.We assessed the safety and immunogenicity of recombinant PfAMA1 in a dose-e...
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2008-01-01
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doaj-5932558cf31e4513b4ca6411ba831dd02020-11-25T01:01:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01312e396010.1371/journal.pone.0003960Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02.Meta RoestenbergEd RemarqueErik de JongeRob HermsenHildur BlythmanOdile LeroyEgeruan ImoukhuedeSoren JepsenOpokua Ofori-AnyinamBart FaberClemens H M KockenMiranda ArnoldVanessa WalravenKarina TeelenWill RoeffenQuirijn de MastW Ripley BallouJoe CohenMarie Claude DuboisStéphane AscarateilAndre van der VenAlan ThomasRobert SauerweinPlasmodium falciparum Apical Membrane Antigen 1 (PfAMA1) is a candidate vaccine antigen expressed by merozoites and sporozoites. It plays a key role in red blood cell and hepatocyte invasion that can be blocked by antibodies.We assessed the safety and immunogenicity of recombinant PfAMA1 in a dose-escalating, phase Ia trial. PfAMA1 FVO strain, produced in Pichia pastoris, was reconstituted at 10 microg and 50 microg doses with three different adjuvants, Alhydrogel, Montanide ISA720 and AS02 Adjuvant System. Six randomised groups of healthy male volunteers, 8-10 volunteers each, were scheduled to receive three immunisations at 4-week intervals. Safety and immunogenicity data were collected over one year. Transient pain was the predominant injection site reaction (80-100%). Induration occurred in the Montanide 50 microg group, resulting in a sterile abscess in two volunteers. Systemic adverse events occurred mainly in the AS02 groups lasting for 1-2 days. Erythema was observed in 22% of Montanide and 59% of AS02 group volunteers. After the second dose, six volunteers in the AS02 group and one in the Montanide group who reported grade 3 erythema (>50 mm) were withdrawn as they met the stopping criteria. All adverse events resolved. There were no vaccine-related serious adverse events. Humoral responses were highest in the AS02 groups. Antibodies showed activity in an in vitro growth inhibition assay up to 80%. Upon stimulation with the vaccine, peripheral mononuclear cells from all groups proliferated and secreted IFNgamma and IL-5 cytokines.All formulations showed distinct reactogenicity profiles. All formulations with PfAMA1 were immunogenic and induced functional antibodies.(Clinicaltrials.gov) NCT00730782.http://europepmc.org/articles/PMC2602972?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meta Roestenberg Ed Remarque Erik de Jonge Rob Hermsen Hildur Blythman Odile Leroy Egeruan Imoukhuede Soren Jepsen Opokua Ofori-Anyinam Bart Faber Clemens H M Kocken Miranda Arnold Vanessa Walraven Karina Teelen Will Roeffen Quirijn de Mast W Ripley Ballou Joe Cohen Marie Claude Dubois Stéphane Ascarateil Andre van der Ven Alan Thomas Robert Sauerwein |
spellingShingle |
Meta Roestenberg Ed Remarque Erik de Jonge Rob Hermsen Hildur Blythman Odile Leroy Egeruan Imoukhuede Soren Jepsen Opokua Ofori-Anyinam Bart Faber Clemens H M Kocken Miranda Arnold Vanessa Walraven Karina Teelen Will Roeffen Quirijn de Mast W Ripley Ballou Joe Cohen Marie Claude Dubois Stéphane Ascarateil Andre van der Ven Alan Thomas Robert Sauerwein Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. PLoS ONE |
author_facet |
Meta Roestenberg Ed Remarque Erik de Jonge Rob Hermsen Hildur Blythman Odile Leroy Egeruan Imoukhuede Soren Jepsen Opokua Ofori-Anyinam Bart Faber Clemens H M Kocken Miranda Arnold Vanessa Walraven Karina Teelen Will Roeffen Quirijn de Mast W Ripley Ballou Joe Cohen Marie Claude Dubois Stéphane Ascarateil Andre van der Ven Alan Thomas Robert Sauerwein |
author_sort |
Meta Roestenberg |
title |
Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. |
title_short |
Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. |
title_full |
Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. |
title_fullStr |
Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. |
title_full_unstemmed |
Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. |
title_sort |
safety and immunogenicity of a recombinant plasmodium falciparum ama1 malaria vaccine adjuvanted with alhydrogel, montanide isa 720 or as02. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-01-01 |
description |
Plasmodium falciparum Apical Membrane Antigen 1 (PfAMA1) is a candidate vaccine antigen expressed by merozoites and sporozoites. It plays a key role in red blood cell and hepatocyte invasion that can be blocked by antibodies.We assessed the safety and immunogenicity of recombinant PfAMA1 in a dose-escalating, phase Ia trial. PfAMA1 FVO strain, produced in Pichia pastoris, was reconstituted at 10 microg and 50 microg doses with three different adjuvants, Alhydrogel, Montanide ISA720 and AS02 Adjuvant System. Six randomised groups of healthy male volunteers, 8-10 volunteers each, were scheduled to receive three immunisations at 4-week intervals. Safety and immunogenicity data were collected over one year. Transient pain was the predominant injection site reaction (80-100%). Induration occurred in the Montanide 50 microg group, resulting in a sterile abscess in two volunteers. Systemic adverse events occurred mainly in the AS02 groups lasting for 1-2 days. Erythema was observed in 22% of Montanide and 59% of AS02 group volunteers. After the second dose, six volunteers in the AS02 group and one in the Montanide group who reported grade 3 erythema (>50 mm) were withdrawn as they met the stopping criteria. All adverse events resolved. There were no vaccine-related serious adverse events. Humoral responses were highest in the AS02 groups. Antibodies showed activity in an in vitro growth inhibition assay up to 80%. Upon stimulation with the vaccine, peripheral mononuclear cells from all groups proliferated and secreted IFNgamma and IL-5 cytokines.All formulations showed distinct reactogenicity profiles. All formulations with PfAMA1 were immunogenic and induced functional antibodies.(Clinicaltrials.gov) NCT00730782. |
url |
http://europepmc.org/articles/PMC2602972?pdf=render |
work_keys_str_mv |
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