A submerged duckweed mutant with abundant starch accumulation for bioethanol production
Abstract Duckweed is one kind of promising bioenergy plant with prominent advantages such as fast growth rate and high starch content. However, almost all previous studies focused on the natural duckweed germplasms. In this study, heavy‐ion irradiation was used to establish a mutant library of Lemna...
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doaj-5937acfb46234de6bb25b9b7d00052be2020-11-25T04:09:46ZengWileyGCB Bioenergy1757-16931757-17072020-12-0112121078109110.1111/gcbb.12746A submerged duckweed mutant with abundant starch accumulation for bioethanol productionYu Liu0Hua Xu1Yu Wang2Xianfeng Tang3Guo He4Shumin Wang5Yubin Ma6Yingzhen Kong7Changjiang Yu8Gongke Zhou9College of Resources and Environment Qingdao Agricultural University Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaCollege of Marine Life Sciences Ocean University of China Qingdao ChinaCollege of Agronomy Qingdao Agricultural University Qingdao ChinaKey Laboratory of Biofuels Qingdao Engineering Research Center of Biomass Resources and Environment Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences Qingdao ChinaCollege of Resources and Environment Qingdao Agricultural University Qingdao ChinaAbstract Duckweed is one kind of promising bioenergy plant with prominent advantages such as fast growth rate and high starch content. However, almost all previous studies focused on the natural duckweed germplasms. In this study, heavy‐ion irradiation was used to establish a mutant library of Lemna aequinoctialis 6002, and one mutant named submarine‐1 (sub‐1) was screened, which could accumulate more starch but with smaller granules. Unexpectedly, under proper external growth conditions such as poor nutritional status and insufficient growth space, sub‐1 mutant would sink underwater due to formation of dense tissue structure and large amount of fine starch particles with the extension of cultivation time. The starch content in the sinking sub‐1 increased to over 45% (dry weight) and was 12% higher than the floating sub‐1, highlighting that submergence can be considered as a spontaneous and efficient indicator for screening of high‐starch duckweed. Additionally, the saccharification efficiency of starch and ethanol yield had increased in sub‐1 mutant compared to the wild type. Based on the unique characteristics of sub‐1 mutant, a cultivation model of submerged duckweed in a simulated aquaculture pond was designed to get more starch‐rich biomass, enabling effective production of renewable bioenergy.https://doi.org/10.1111/gcbb.12746bioethanolduckweedheavy‐ion irradiationstarchsub‐1 mutantsubmergence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Liu Hua Xu Yu Wang Xianfeng Tang Guo He Shumin Wang Yubin Ma Yingzhen Kong Changjiang Yu Gongke Zhou |
spellingShingle |
Yu Liu Hua Xu Yu Wang Xianfeng Tang Guo He Shumin Wang Yubin Ma Yingzhen Kong Changjiang Yu Gongke Zhou A submerged duckweed mutant with abundant starch accumulation for bioethanol production GCB Bioenergy bioethanol duckweed heavy‐ion irradiation starch sub‐1 mutant submergence |
author_facet |
Yu Liu Hua Xu Yu Wang Xianfeng Tang Guo He Shumin Wang Yubin Ma Yingzhen Kong Changjiang Yu Gongke Zhou |
author_sort |
Yu Liu |
title |
A submerged duckweed mutant with abundant starch accumulation for bioethanol production |
title_short |
A submerged duckweed mutant with abundant starch accumulation for bioethanol production |
title_full |
A submerged duckweed mutant with abundant starch accumulation for bioethanol production |
title_fullStr |
A submerged duckweed mutant with abundant starch accumulation for bioethanol production |
title_full_unstemmed |
A submerged duckweed mutant with abundant starch accumulation for bioethanol production |
title_sort |
submerged duckweed mutant with abundant starch accumulation for bioethanol production |
publisher |
Wiley |
series |
GCB Bioenergy |
issn |
1757-1693 1757-1707 |
publishDate |
2020-12-01 |
description |
Abstract Duckweed is one kind of promising bioenergy plant with prominent advantages such as fast growth rate and high starch content. However, almost all previous studies focused on the natural duckweed germplasms. In this study, heavy‐ion irradiation was used to establish a mutant library of Lemna aequinoctialis 6002, and one mutant named submarine‐1 (sub‐1) was screened, which could accumulate more starch but with smaller granules. Unexpectedly, under proper external growth conditions such as poor nutritional status and insufficient growth space, sub‐1 mutant would sink underwater due to formation of dense tissue structure and large amount of fine starch particles with the extension of cultivation time. The starch content in the sinking sub‐1 increased to over 45% (dry weight) and was 12% higher than the floating sub‐1, highlighting that submergence can be considered as a spontaneous and efficient indicator for screening of high‐starch duckweed. Additionally, the saccharification efficiency of starch and ethanol yield had increased in sub‐1 mutant compared to the wild type. Based on the unique characteristics of sub‐1 mutant, a cultivation model of submerged duckweed in a simulated aquaculture pond was designed to get more starch‐rich biomass, enabling effective production of renewable bioenergy. |
topic |
bioethanol duckweed heavy‐ion irradiation starch sub‐1 mutant submergence |
url |
https://doi.org/10.1111/gcbb.12746 |
work_keys_str_mv |
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