Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
Abstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocard...
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doaj-59415c078eb549519737283144b3bd142020-12-08T00:33:46ZengNature Publishing GroupScientific Reports2045-23222017-08-017111110.1038/s41598-017-08546-1Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathySahoko Ichihara0Yuka Suzuki1Jie Chang2Kentaro Kuzuya3Chisa Inoue4Yuki Kitamura5Shinji Oikawa6Graduate School of Regional Innovation StudiesGraduate School of Regional Innovation StudiesGraduate School of Regional Innovation StudiesDepartment of Human Functional Genomics, Life Science Research Center, Mie UniversityDepartment of Human Functional Genomics, Life Science Research Center, Mie UniversityDepartment of Environmental and Molecular Medicine, Mie University Graduate School of MedicineDepartment of Environmental and Molecular Medicine, Mie University Graduate School of MedicineAbstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocardial proteins in the whole left ventricles between 6-week-old hamsters with dilated (TO-2) and hypertrophic cardiomyopathy (Bio14.6) and control hamsters (F1B). Then, 2D electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry detected 18 proteins with increased carbonyl level in cardiomyopathy hamsters compared with control hamster. Carbonyl modification of proteins related to ATP synthesis, including citric acid cycle and electron transport system, was observed in the hearts of hamsters with both types of cardiomyopathy. Further analysis indicated that left ventricular carbonyl production correlated negatively with succinyl-CoA:3-ketoacid-coenzyme A transferase 1 activity (r 2 = 0.60, P = 0.0007) and ATP concentration (r 2 = 0.29, P = 0.037), suggesting that protein carbonylation has negative effects on the levels of these biomolecules. Furthermore, carbonyl production significantly correlated with plasma Troponin T level (r 2 = 0.33, P = 0.026). Reduction of energy metabolism by oxidative damage may contribute to the development of left ventricular impairment in cardiomyopathy.https://doi.org/10.1038/s41598-017-08546-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa |
spellingShingle |
Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy Scientific Reports |
author_facet |
Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa |
author_sort |
Sahoko Ichihara |
title |
Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy |
title_short |
Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy |
title_full |
Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy |
title_fullStr |
Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy |
title_full_unstemmed |
Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy |
title_sort |
involvement of oxidative modification of proteins related to atp synthesis in the left ventricles of hamsters with cardiomyopathy |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocardial proteins in the whole left ventricles between 6-week-old hamsters with dilated (TO-2) and hypertrophic cardiomyopathy (Bio14.6) and control hamsters (F1B). Then, 2D electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry detected 18 proteins with increased carbonyl level in cardiomyopathy hamsters compared with control hamster. Carbonyl modification of proteins related to ATP synthesis, including citric acid cycle and electron transport system, was observed in the hearts of hamsters with both types of cardiomyopathy. Further analysis indicated that left ventricular carbonyl production correlated negatively with succinyl-CoA:3-ketoacid-coenzyme A transferase 1 activity (r 2 = 0.60, P = 0.0007) and ATP concentration (r 2 = 0.29, P = 0.037), suggesting that protein carbonylation has negative effects on the levels of these biomolecules. Furthermore, carbonyl production significantly correlated with plasma Troponin T level (r 2 = 0.33, P = 0.026). Reduction of energy metabolism by oxidative damage may contribute to the development of left ventricular impairment in cardiomyopathy. |
url |
https://doi.org/10.1038/s41598-017-08546-1 |
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