The Influence of Explant Types and Orientation on in Vitro Culture

The Influence of Explant Types and Orientation on in Vitro Culture

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Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surfac...

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Main Authors: Kamnoon KANCHANAPOOM, Nararatn PROMSORN
Format: Article
Language:English
Published: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 2011-08-01
Series:Notulae Scientia Biologicae
Online Access:http://www.notulaebiologicae.ro/index.php/nsb/article/view/6120
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spelling doaj-594a7ef39e484cc4b55bc7f50df7545b2020-11-25T02:03:27ZengUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-NapocaNotulae Scientia Biologicae2067-32052067-32642011-08-0133899210.15835/nsb3361206029The Influence of Explant Types and Orientation on in Vitro CultureKamnoon KANCHANAPOOM0Nararatn PROMSORN1Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla 90112Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.http://www.notulaebiologicae.ro/index.php/nsb/article/view/6120
collection DOAJ
language English
format Article
sources DOAJ
author Kamnoon KANCHANAPOOM
Nararatn PROMSORN
spellingShingle Kamnoon KANCHANAPOOM
Nararatn PROMSORN
The Influence of Explant Types and Orientation on in Vitro Culture
Notulae Scientia Biologicae
author_facet Kamnoon KANCHANAPOOM
Nararatn PROMSORN
author_sort Kamnoon KANCHANAPOOM
title The Influence of Explant Types and Orientation on in Vitro Culture
title_short The Influence of Explant Types and Orientation on in Vitro Culture
title_full The Influence of Explant Types and Orientation on in Vitro Culture
title_fullStr The Influence of Explant Types and Orientation on in Vitro Culture
title_full_unstemmed The Influence of Explant Types and Orientation on in Vitro Culture
title_sort influence of explant types and orientation on in vitro culture
publisher University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
series Notulae Scientia Biologicae
issn 2067-3205
2067-3264
publishDate 2011-08-01
description Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.
url http://www.notulaebiologicae.ro/index.php/nsb/article/view/6120
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AT kamnoonkanchanapoom influenceofexplanttypesandorientationoninvitroculture
AT nararatnpromsorn influenceofexplanttypesandorientationoninvitroculture
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