Cell Electrosensitization Exists Only in Certain Electroporation Buffers.
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be e...
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doaj-5952efe560d24f9da9089303cce0bb7c2020-11-25T00:40:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015943410.1371/journal.pone.0159434Cell Electrosensitization Exists Only in Certain Electroporation Buffers.Janja DermolOlga N PakhomovaAndrei G PakhomovDamijan MiklavčičElectroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified. We investigated cell sensitization dynamics in five different electroporation buffers. We split a pulse train into two trains varying the delay between them and measured the propidium uptake by fluorescence microscopy. By fitting the first-order model to the experimental results, we determined the uptake due to each train (i.e. the first and the second) and the corresponding resealing constant. Cell sensitization was observed in the growth medium but not in other tested buffers. The effect of pulse repetition frequency, cell size change, cytoskeleton disruption and calcium influx do not adequately explain cell sensitization. Based on our results, we can conclude that cell sensitization is a sum of several processes and is buffer dependent. Further research is needed to determine its generality and to identify underlying mechanisms.http://europepmc.org/articles/PMC4959715?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Janja Dermol Olga N Pakhomova Andrei G Pakhomov Damijan Miklavčič |
spellingShingle |
Janja Dermol Olga N Pakhomova Andrei G Pakhomov Damijan Miklavčič Cell Electrosensitization Exists Only in Certain Electroporation Buffers. PLoS ONE |
author_facet |
Janja Dermol Olga N Pakhomova Andrei G Pakhomov Damijan Miklavčič |
author_sort |
Janja Dermol |
title |
Cell Electrosensitization Exists Only in Certain Electroporation Buffers. |
title_short |
Cell Electrosensitization Exists Only in Certain Electroporation Buffers. |
title_full |
Cell Electrosensitization Exists Only in Certain Electroporation Buffers. |
title_fullStr |
Cell Electrosensitization Exists Only in Certain Electroporation Buffers. |
title_full_unstemmed |
Cell Electrosensitization Exists Only in Certain Electroporation Buffers. |
title_sort |
cell electrosensitization exists only in certain electroporation buffers. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified. We investigated cell sensitization dynamics in five different electroporation buffers. We split a pulse train into two trains varying the delay between them and measured the propidium uptake by fluorescence microscopy. By fitting the first-order model to the experimental results, we determined the uptake due to each train (i.e. the first and the second) and the corresponding resealing constant. Cell sensitization was observed in the growth medium but not in other tested buffers. The effect of pulse repetition frequency, cell size change, cytoskeleton disruption and calcium influx do not adequately explain cell sensitization. Based on our results, we can conclude that cell sensitization is a sum of several processes and is buffer dependent. Further research is needed to determine its generality and to identify underlying mechanisms. |
url |
http://europepmc.org/articles/PMC4959715?pdf=render |
work_keys_str_mv |
AT janjadermol cellelectrosensitizationexistsonlyincertainelectroporationbuffers AT olganpakhomova cellelectrosensitizationexistsonlyincertainelectroporationbuffers AT andreigpakhomov cellelectrosensitizationexistsonlyincertainelectroporationbuffers AT damijanmiklavcic cellelectrosensitizationexistsonlyincertainelectroporationbuffers |
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