Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology
Among noncoding RNA sequences, riboswitches and ribozymes have attracted the attention of the synthetic biology community as circuit components for translation regulation. When fused to aptamer sequences, ribozymes and riboswitches are enabled to interact with chemicals. Therefore, protein synthesis...
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doaj-5958e04071f84502ba5eced1b0d9e29c2021-03-18T00:02:26ZengMDPI AGLife2075-17292021-03-011124824810.3390/life11030248Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic BiologyHuanhuan Ge0Mario Andrea Marchisio1School of Pharmaceutical Science and Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, ChinaSchool of Pharmaceutical Science and Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, ChinaAmong noncoding RNA sequences, riboswitches and ribozymes have attracted the attention of the synthetic biology community as circuit components for translation regulation. When fused to aptamer sequences, ribozymes and riboswitches are enabled to interact with chemicals. Therefore, protein synthesis can be controlled at the mRNA level without the need for transcription factors. Potentially, the use of chemical-responsive ribozymes/riboswitches would drastically simplify the design of genetic circuits. In this review, we describe synthetic RNA structures that have been used so far in the yeast <i>Saccharomyces cerevisiae</i>. We present their interaction mode with different chemicals (e.g., theophylline and antibiotics) or proteins (such as the RNase III) and their recent employment into clustered regularly interspaced short palindromic repeats–CRISPR-associated protein 9 (CRISPR-Cas) systems. Particular attention is paid, throughout the whole paper, to their usage and performance into synthetic gene circuits.https://www.mdpi.com/2075-1729/11/3/248<i>S. cerevisiae</i>aptamersriboswitchesribozymessynthetic biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huanhuan Ge Mario Andrea Marchisio |
spellingShingle |
Huanhuan Ge Mario Andrea Marchisio Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology Life <i>S. cerevisiae</i> aptamers riboswitches ribozymes synthetic biology |
author_facet |
Huanhuan Ge Mario Andrea Marchisio |
author_sort |
Huanhuan Ge |
title |
Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology |
title_short |
Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology |
title_full |
Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology |
title_fullStr |
Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology |
title_full_unstemmed |
Aptamers, Riboswitches and Ribozymes in <i>S. cerevisiae </i>Synthetic Biology |
title_sort |
aptamers, riboswitches and ribozymes in <i>s. cerevisiae </i>synthetic biology |
publisher |
MDPI AG |
series |
Life |
issn |
2075-1729 |
publishDate |
2021-03-01 |
description |
Among noncoding RNA sequences, riboswitches and ribozymes have attracted the attention of the synthetic biology community as circuit components for translation regulation. When fused to aptamer sequences, ribozymes and riboswitches are enabled to interact with chemicals. Therefore, protein synthesis can be controlled at the mRNA level without the need for transcription factors. Potentially, the use of chemical-responsive ribozymes/riboswitches would drastically simplify the design of genetic circuits. In this review, we describe synthetic RNA structures that have been used so far in the yeast <i>Saccharomyces cerevisiae</i>. We present their interaction mode with different chemicals (e.g., theophylline and antibiotics) or proteins (such as the RNase III) and their recent employment into clustered regularly interspaced short palindromic repeats–CRISPR-associated protein 9 (CRISPR-Cas) systems. Particular attention is paid, throughout the whole paper, to their usage and performance into synthetic gene circuits. |
topic |
<i>S. cerevisiae</i> aptamers riboswitches ribozymes synthetic biology |
url |
https://www.mdpi.com/2075-1729/11/3/248 |
work_keys_str_mv |
AT huanhuange aptamersriboswitchesandribozymesiniscerevisiaeisyntheticbiology AT marioandreamarchisio aptamersriboswitchesandribozymesiniscerevisiaeisyntheticbiology |
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