Mmp17b is essential for proper neural crest cell migration in vivo.
The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b f...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3788140?pdf=render |
id |
doaj-5976424d5d9448498361d5d806c8403d |
---|---|
record_format |
Article |
spelling |
doaj-5976424d5d9448498361d5d806c8403d2020-11-24T20:45:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7648410.1371/journal.pone.0076484Mmp17b is essential for proper neural crest cell migration in vivo.Noah R LeighMarcus-Oliver SchuppKeguo LiVakeel PadmanabhanAdam GastonguayLing WangChang Z ChunGeorge A WilkinsonRamani RamchandranThe extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b function, or chemical inhibition of MMP activity results in aberrant NC cell migration with minimal change in NC proliferation or apoptosis. Intriguingly, a GPI anchored protein with metalloproteinase inhibitor properties, Reversion-inducing-Cysteine-rich protein with Kazal motifs (RECK), which has previously been implicated in NC development, is expressed in close apposition to NC cells expressing mmp17b, raising the possibility that these two gene products interact. Consistent with this possibility, embryos silenced for mmp17b show defective development of the dorsal root ganglia (DRG), a crest-derived structure affected in RECK mutant fish sensory deprived (sdp). Taken together, this study has identified the first pair of MMP, and their putative MMP inhibitor RECK that functions together in NC cell migration.http://europepmc.org/articles/PMC3788140?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noah R Leigh Marcus-Oliver Schupp Keguo Li Vakeel Padmanabhan Adam Gastonguay Ling Wang Chang Z Chun George A Wilkinson Ramani Ramchandran |
spellingShingle |
Noah R Leigh Marcus-Oliver Schupp Keguo Li Vakeel Padmanabhan Adam Gastonguay Ling Wang Chang Z Chun George A Wilkinson Ramani Ramchandran Mmp17b is essential for proper neural crest cell migration in vivo. PLoS ONE |
author_facet |
Noah R Leigh Marcus-Oliver Schupp Keguo Li Vakeel Padmanabhan Adam Gastonguay Ling Wang Chang Z Chun George A Wilkinson Ramani Ramchandran |
author_sort |
Noah R Leigh |
title |
Mmp17b is essential for proper neural crest cell migration in vivo. |
title_short |
Mmp17b is essential for proper neural crest cell migration in vivo. |
title_full |
Mmp17b is essential for proper neural crest cell migration in vivo. |
title_fullStr |
Mmp17b is essential for proper neural crest cell migration in vivo. |
title_full_unstemmed |
Mmp17b is essential for proper neural crest cell migration in vivo. |
title_sort |
mmp17b is essential for proper neural crest cell migration in vivo. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b function, or chemical inhibition of MMP activity results in aberrant NC cell migration with minimal change in NC proliferation or apoptosis. Intriguingly, a GPI anchored protein with metalloproteinase inhibitor properties, Reversion-inducing-Cysteine-rich protein with Kazal motifs (RECK), which has previously been implicated in NC development, is expressed in close apposition to NC cells expressing mmp17b, raising the possibility that these two gene products interact. Consistent with this possibility, embryos silenced for mmp17b show defective development of the dorsal root ganglia (DRG), a crest-derived structure affected in RECK mutant fish sensory deprived (sdp). Taken together, this study has identified the first pair of MMP, and their putative MMP inhibitor RECK that functions together in NC cell migration. |
url |
http://europepmc.org/articles/PMC3788140?pdf=render |
work_keys_str_mv |
AT noahrleigh mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT marcusoliverschupp mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT keguoli mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT vakeelpadmanabhan mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT adamgastonguay mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT lingwang mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT changzchun mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT georgeawilkinson mmp17bisessentialforproperneuralcrestcellmigrationinvivo AT ramaniramchandran mmp17bisessentialforproperneuralcrestcellmigrationinvivo |
_version_ |
1716813827563061248 |