Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture
A major concern associated with the use of drugs is their adverse side effects. Specific examples of the drugs of concern include antibiotic agents and non-steroidal anti-inflammatory drugs. Despite the presence of a high degree of efficacy for specific conditions, these drugs may deteriorate the su...
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doaj-5976f018ca2d483c8d82cc4b88f877062020-11-25T01:23:21ZengPeerJ Inc.PeerJ2167-83592020-01-018e835510.7717/peerj.8355Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte cultureKorakot Nganvongpanit0Thippaporn Euppayo1Puntita Siengdee2Kittisak Buddhachat3Siriwadee Chomdej4Siriwan Ongchai5Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandAnimal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandAnimal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandExcellence Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, ThailandExcellence Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandA major concern associated with the use of drugs is their adverse side effects. Specific examples of the drugs of concern include antibiotic agents and non-steroidal anti-inflammatory drugs. Despite the presence of a high degree of efficacy for specific conditions, these drugs may deteriorate the surrounding tissues that are exposed to them. Often, carprofen is used for joint inflammation; however, it may stimulate cartilage degradation which can then lead to osteoarthritis progression. In this study, hyaluronan was combined with carprofen treatment in three different applications (pre-treatment, co-treatment and post-treatment) on normal canine chondrocytes to determine whether Hyaluronan (HA) is capable of mitigating the degree of chondrotoxicity of carprofen. Our findings revealed that carprofen at IC20 (0.16 mg/mL) decreased viability and increased nitric oxide (NO) production. Importantly, carprofen induced the apoptosis of canine chondrocytes via the up-regulation of Bax, Casp3, Casp8, Casp9 and NOS2 as compared to the control group. Although the co-treatment of HA and carprofen appeared not to further alleviate the chondrotoxicity of carprofen due to the presence of a high number of apoptotic chondrocytes, post-treatment with HA (carprofen treatment for 24 h and then changed to HA for 24 h) resulted in a decrease in chondrocyte apoptosis by the down-regulation of Bax, Casp3, Casp8, Casp9, NOS2, along with NO production when compared with the treatment of carprofen for 48 h (P < 0.05). These results suggest that HA can be used as a therapeutic agent to mitigate the degree of chondrotoxicity of carprofen.https://peerj.com/articles/8355.pdfApoptosisCarprofenHyaluronic acidCellChondrocyte |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Korakot Nganvongpanit Thippaporn Euppayo Puntita Siengdee Kittisak Buddhachat Siriwadee Chomdej Siriwan Ongchai |
spellingShingle |
Korakot Nganvongpanit Thippaporn Euppayo Puntita Siengdee Kittisak Buddhachat Siriwadee Chomdej Siriwan Ongchai Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture PeerJ Apoptosis Carprofen Hyaluronic acid Cell Chondrocyte |
author_facet |
Korakot Nganvongpanit Thippaporn Euppayo Puntita Siengdee Kittisak Buddhachat Siriwadee Chomdej Siriwan Ongchai |
author_sort |
Korakot Nganvongpanit |
title |
Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
title_short |
Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
title_full |
Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
title_fullStr |
Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
title_full_unstemmed |
Post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
title_sort |
post-treatment of hyaluronan to decrease the apoptotic effects of carprofen in canine articular chondrocyte culture |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2020-01-01 |
description |
A major concern associated with the use of drugs is their adverse side effects. Specific examples of the drugs of concern include antibiotic agents and non-steroidal anti-inflammatory drugs. Despite the presence of a high degree of efficacy for specific conditions, these drugs may deteriorate the surrounding tissues that are exposed to them. Often, carprofen is used for joint inflammation; however, it may stimulate cartilage degradation which can then lead to osteoarthritis progression. In this study, hyaluronan was combined with carprofen treatment in three different applications (pre-treatment, co-treatment and post-treatment) on normal canine chondrocytes to determine whether Hyaluronan (HA) is capable of mitigating the degree of chondrotoxicity of carprofen. Our findings revealed that carprofen at IC20 (0.16 mg/mL) decreased viability and increased nitric oxide (NO) production. Importantly, carprofen induced the apoptosis of canine chondrocytes via the up-regulation of Bax, Casp3, Casp8, Casp9 and NOS2 as compared to the control group. Although the co-treatment of HA and carprofen appeared not to further alleviate the chondrotoxicity of carprofen due to the presence of a high number of apoptotic chondrocytes, post-treatment with HA (carprofen treatment for 24 h and then changed to HA for 24 h) resulted in a decrease in chondrocyte apoptosis by the down-regulation of Bax, Casp3, Casp8, Casp9, NOS2, along with NO production when compared with the treatment of carprofen for 48 h (P < 0.05). These results suggest that HA can be used as a therapeutic agent to mitigate the degree of chondrotoxicity of carprofen. |
topic |
Apoptosis Carprofen Hyaluronic acid Cell Chondrocyte |
url |
https://peerj.com/articles/8355.pdf |
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