Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis
The present study evaluated the pheno- and genotypical antimicrobial resistance profile of coagulase-negative Staphylococcus (CNS) species isolated from dairy cows milk, specially concerning to oxacillin. Of 100 CNS isolates, the S. xylosus was the prevalent species, followed by S. cohnii, S. homini...
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Colégio Brasileiro de Patologia Animal (CBPA)
2012-08-01
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doaj-597a11bf5df3444898832f39dd1e60e82020-11-25T01:13:43ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502012-08-0132869269610.1590/S0100-736X2012000800002S0100-736X2012000800002Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitisLidiane C. Soares0Ingrid A. Pereira1Bruno R. Pribul2Marcelo S. Oliva3Shana M.O. Coelho4Miliane M.S. Souza5Universidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroThe present study evaluated the pheno- and genotypical antimicrobial resistance profile of coagulase-negative Staphylococcus (CNS) species isolated from dairy cows milk, specially concerning to oxacillin. Of 100 CNS isolates, the S. xylosus was the prevalent species, followed by S. cohnii, S. hominis, S. capitis and S. haemolyticus. Only 6% were phenotypically susceptible to the antimicrobial agents tested in disk diffusion assay. Penicillin and ampicillin resistance rates were significantly higher than others antimicrobials. Four isolates were positive to mecA gene (4%), all represented by the S. xylosus species. The blaZ gene was detected in 16% of the isolates (16/100). It was noticed that all mecA + were also positive to this gene and the presence of both genes was correlated to phenotypic beta-lactamic resistance. We conclude that CNS species from bovine milk presented significantly distinct antimicrobial resistance profiles, evaluated by phenotypic and genotypic tests, which has implications for treatment and management decisions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000800002&lng=en&tlng=enMastiteestafilococos coagulase negativosresistência antimicrobianagene mecAgene blaZbovinos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lidiane C. Soares Ingrid A. Pereira Bruno R. Pribul Marcelo S. Oliva Shana M.O. Coelho Miliane M.S. Souza |
spellingShingle |
Lidiane C. Soares Ingrid A. Pereira Bruno R. Pribul Marcelo S. Oliva Shana M.O. Coelho Miliane M.S. Souza Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis Pesquisa Veterinária Brasileira Mastite estafilococos coagulase negativos resistência antimicrobiana gene mecA gene blaZ bovinos |
author_facet |
Lidiane C. Soares Ingrid A. Pereira Bruno R. Pribul Marcelo S. Oliva Shana M.O. Coelho Miliane M.S. Souza |
author_sort |
Lidiane C. Soares |
title |
Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis |
title_short |
Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis |
title_full |
Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis |
title_fullStr |
Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis |
title_full_unstemmed |
Antimicrobial resistance and detection of mecA and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis |
title_sort |
antimicrobial resistance and detection of meca and blaz genes in coagulase-negative staphylococcus isolated from bovine mastitis |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
publishDate |
2012-08-01 |
description |
The present study evaluated the pheno- and genotypical antimicrobial resistance profile of coagulase-negative Staphylococcus (CNS) species isolated from dairy cows milk, specially concerning to oxacillin. Of 100 CNS isolates, the S. xylosus was the prevalent species, followed by S. cohnii, S. hominis, S. capitis and S. haemolyticus. Only 6% were phenotypically susceptible to the antimicrobial agents tested in disk diffusion assay. Penicillin and ampicillin resistance rates were significantly higher than others antimicrobials. Four isolates were positive to mecA gene (4%), all represented by the S. xylosus species. The blaZ gene was detected in 16% of the isolates (16/100). It was noticed that all mecA + were also positive to this gene and the presence of both genes was correlated to phenotypic beta-lactamic resistance. We conclude that CNS species from bovine milk presented significantly distinct antimicrobial resistance profiles, evaluated by phenotypic and genotypic tests, which has implications for treatment and management decisions. |
topic |
Mastite estafilococos coagulase negativos resistência antimicrobiana gene mecA gene blaZ bovinos |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000800002&lng=en&tlng=en |
work_keys_str_mv |
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