gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene
Intramuscular fat (IMF) is the most important evaluating indicator of chicken meat quality, the content of which is positively correlated with tenderness, flavor, and succulence of the meat. Chicken IMF deposition process is regulated by many factors, including genetic, nutrition, and environment. A...
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doaj-59ba3fc927ba4e95abd205f8f359bdcb2020-11-25T00:25:59ZengMDPI AGCells2073-44092019-06-018655610.3390/cells8060556cells8060556gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> GeneGuirong Sun0Fang Li1Xiangfei Ma2Junwei Sun3Ruirui Jiang4Yadong Tian5Ruili Han6Guoxi Li7Yanbin Wang8Zhuanjian Li9Xiangtao Kang10Wenting Li11College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaIntramuscular fat (IMF) is the most important evaluating indicator of chicken meat quality, the content of which is positively correlated with tenderness, flavor, and succulence of the meat. Chicken IMF deposition process is regulated by many factors, including genetic, nutrition, and environment. Although large number of omics’ studies focused on the IMF deposition process, the molecular mechanism of chicken IMF deposition is still poorly understood. In order to study the role of miRNAs in chicken intramuscular adipogenesis, the intramuscular adipocyte differentiation model (IMF-preadipocytes and IMF-adipocytes) was established and subject to miRNA-Seq. A total of 117 differentially expressed miRNAs between two groups were obtained. Target genes prediction and functional enrichment analysis revealed that eight pathways involved in lipid metabolism related processes, such as fatty acid metabolism and fatty acid elongation. Meanwhile a putative miRNA, gga-miR-18b-3p, was identified be served a function in the intramuscular adipocyte differentiation. Luciferase assay suggested that the gga-miR-18b-3p targeted to the 3′UTR of <i>ACOT13</i>. Subsequent functional experiments demonstrated that gga-miR-18b-3p acted as an inhibitor of intramuscular adipocyte differentiation by targeting <i>ACOT13</i>. Our findings laid a new theoretical foundation for the study of lipid metabolism, and also provided a potential target to improve the meat quality in the poultry industry.https://www.mdpi.com/2073-4409/8/6/556miR-18b-3p<i>ACOT13</i>intramuscular adipocytesdifferentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guirong Sun Fang Li Xiangfei Ma Junwei Sun Ruirui Jiang Yadong Tian Ruili Han Guoxi Li Yanbin Wang Zhuanjian Li Xiangtao Kang Wenting Li |
spellingShingle |
Guirong Sun Fang Li Xiangfei Ma Junwei Sun Ruirui Jiang Yadong Tian Ruili Han Guoxi Li Yanbin Wang Zhuanjian Li Xiangtao Kang Wenting Li gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene Cells miR-18b-3p <i>ACOT13</i> intramuscular adipocytes differentiation |
author_facet |
Guirong Sun Fang Li Xiangfei Ma Junwei Sun Ruirui Jiang Yadong Tian Ruili Han Guoxi Li Yanbin Wang Zhuanjian Li Xiangtao Kang Wenting Li |
author_sort |
Guirong Sun |
title |
gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene |
title_short |
gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene |
title_full |
gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene |
title_fullStr |
gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene |
title_full_unstemmed |
gga-miRNA-18b-3p Inhibits Intramuscular Adipocytes Differentiation in Chicken by Targeting the <i>ACOT13</i> Gene |
title_sort |
gga-mirna-18b-3p inhibits intramuscular adipocytes differentiation in chicken by targeting the <i>acot13</i> gene |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-06-01 |
description |
Intramuscular fat (IMF) is the most important evaluating indicator of chicken meat quality, the content of which is positively correlated with tenderness, flavor, and succulence of the meat. Chicken IMF deposition process is regulated by many factors, including genetic, nutrition, and environment. Although large number of omics’ studies focused on the IMF deposition process, the molecular mechanism of chicken IMF deposition is still poorly understood. In order to study the role of miRNAs in chicken intramuscular adipogenesis, the intramuscular adipocyte differentiation model (IMF-preadipocytes and IMF-adipocytes) was established and subject to miRNA-Seq. A total of 117 differentially expressed miRNAs between two groups were obtained. Target genes prediction and functional enrichment analysis revealed that eight pathways involved in lipid metabolism related processes, such as fatty acid metabolism and fatty acid elongation. Meanwhile a putative miRNA, gga-miR-18b-3p, was identified be served a function in the intramuscular adipocyte differentiation. Luciferase assay suggested that the gga-miR-18b-3p targeted to the 3′UTR of <i>ACOT13</i>. Subsequent functional experiments demonstrated that gga-miR-18b-3p acted as an inhibitor of intramuscular adipocyte differentiation by targeting <i>ACOT13</i>. Our findings laid a new theoretical foundation for the study of lipid metabolism, and also provided a potential target to improve the meat quality in the poultry industry. |
topic |
miR-18b-3p <i>ACOT13</i> intramuscular adipocytes differentiation |
url |
https://www.mdpi.com/2073-4409/8/6/556 |
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