Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae
Polycomb (PcG) and Trithorax (TrxG) complexes are two evolutionarily conserved epigenetic regulatory components that act antagonistically to regulate the expression of genes involved in cell differentiation and development in multicellular organisms. The absence of PcG in both yeast models Saccharom...
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doaj-59c5779899dc4f74abcd1988d70dea7a2020-11-25T03:15:39ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-03-01710.3389/fmars.2020.00189518805Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled MicroalgaeXue Zhao0Xue Zhao1Anne Flore Deton Cabanillas2Alaguraj Veluchamy3Chris Bowler4Fabio Rocha Jimenez Vieira5Leila Tirichine6Department of Biology, Université de Nantes, CNRS, UFIP, UMR 6286, Nantes, FranceUniversit Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), Orsay, FranceInstitut de Biologie de l’Ecolenormale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Université Paris, Paris, FranceBiological and Environmental Science and Engineering Division, Laboratory of Chromatin Biochemistry, King Abdullah University of Science and Technology, Thuwal, Saudi ArabiaInstitut de Biologie de l’Ecolenormale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Université Paris, Paris, FranceInstitut de Biologie de l’Ecolenormale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Université Paris, Paris, FranceDepartment of Biology, Université de Nantes, CNRS, UFIP, UMR 6286, Nantes, FrancePolycomb (PcG) and Trithorax (TrxG) complexes are two evolutionarily conserved epigenetic regulatory components that act antagonistically to regulate the expression of genes involved in cell differentiation and development in multicellular organisms. The absence of PcG in both yeast models Saccharomyces cerevisiae and Schizosaccharomyces pombe suggested that polycomb proteins might have evolved together with the emergence of multicellular organisms. However, high throughput sequencing of several microalgal genomes and transcriptomes reveals an unprecedented abundance and diversity of genes encoding the components of these complexes. We report here the diversity of genes encoding PcG and TrxG proteins in microalgae from the Marine Microbial Eukaryote Transcriptome Sequencing Project database (MMETSP) and detected at broad scale in Tara Oceans genomics datasets using a highly sensitive method called eDAF (enhanced Domain Architecture Filtering). Further, we explored the correlation between environmental factors measured during the Tara Oceans expedition and transcript levels of PcG and TrxG components. PcG and TrxG are responsible for the deposition of a number of histone marks among which a TrxG associated mark, H3K4me3 which we profiled genome wide in the model diatom Phaeodactylum tricornutum to understand its role in microalgae and revisited the previously published histone code and co-occurrence with other histone marks including the antagonizing Polycomb deposited mark H3K27me3.https://www.frontiersin.org/article/10.3389/fmars.2020.00189/fullepigeneticsenvironmentmicroalgaediatomsbioinformaticsPhaeodactylum tricornutum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xue Zhao Xue Zhao Anne Flore Deton Cabanillas Alaguraj Veluchamy Chris Bowler Fabio Rocha Jimenez Vieira Leila Tirichine |
spellingShingle |
Xue Zhao Xue Zhao Anne Flore Deton Cabanillas Alaguraj Veluchamy Chris Bowler Fabio Rocha Jimenez Vieira Leila Tirichine Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae Frontiers in Marine Science epigenetics environment microalgae diatoms bioinformatics Phaeodactylum tricornutum |
author_facet |
Xue Zhao Xue Zhao Anne Flore Deton Cabanillas Alaguraj Veluchamy Chris Bowler Fabio Rocha Jimenez Vieira Leila Tirichine |
author_sort |
Xue Zhao |
title |
Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae |
title_short |
Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae |
title_full |
Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae |
title_fullStr |
Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae |
title_full_unstemmed |
Probing the Diversity of Polycomb and Trithorax Proteins in Cultured and Environmentally Sampled Microalgae |
title_sort |
probing the diversity of polycomb and trithorax proteins in cultured and environmentally sampled microalgae |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Marine Science |
issn |
2296-7745 |
publishDate |
2020-03-01 |
description |
Polycomb (PcG) and Trithorax (TrxG) complexes are two evolutionarily conserved epigenetic regulatory components that act antagonistically to regulate the expression of genes involved in cell differentiation and development in multicellular organisms. The absence of PcG in both yeast models Saccharomyces cerevisiae and Schizosaccharomyces pombe suggested that polycomb proteins might have evolved together with the emergence of multicellular organisms. However, high throughput sequencing of several microalgal genomes and transcriptomes reveals an unprecedented abundance and diversity of genes encoding the components of these complexes. We report here the diversity of genes encoding PcG and TrxG proteins in microalgae from the Marine Microbial Eukaryote Transcriptome Sequencing Project database (MMETSP) and detected at broad scale in Tara Oceans genomics datasets using a highly sensitive method called eDAF (enhanced Domain Architecture Filtering). Further, we explored the correlation between environmental factors measured during the Tara Oceans expedition and transcript levels of PcG and TrxG components. PcG and TrxG are responsible for the deposition of a number of histone marks among which a TrxG associated mark, H3K4me3 which we profiled genome wide in the model diatom Phaeodactylum tricornutum to understand its role in microalgae and revisited the previously published histone code and co-occurrence with other histone marks including the antagonizing Polycomb deposited mark H3K27me3. |
topic |
epigenetics environment microalgae diatoms bioinformatics Phaeodactylum tricornutum |
url |
https://www.frontiersin.org/article/10.3389/fmars.2020.00189/full |
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