Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior
Low-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompa...
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doaj-59d9e899cfab4e96ad6a2168bfbdd8f52020-11-25T01:14:21ZengMDPI AGCoatings2079-64122019-02-019213410.3390/coatings9020134coatings9020134Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell BehaviorCheuk Sing Choy0Eisner Salamanca1Pei Ying Lin2Haw-Ming Huang3Nai-Chia Teng4Yu-Hwa Pan5Wei-Jen Chang6Department of Community Medicine, En Chu Kong Hospital, New Taipei City 237, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Community Medicine, En Chu Kong Hospital, New Taipei City 237, TaiwanDepartment of Community Medicine, En Chu Kong Hospital, New Taipei City 237, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanLow-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompatibility in-vitro. Our results showed that PGPT did not differ in roughness, dominant crystalline phases, absorption peaks corresponding to phosphate band peaks, or micro-meso pore size, compared to non-treated porcine grafts. The PGPT Ca/P ratio was 2.16; whereas the porcine control ratio was 2.04 (<i>p</i> < 0.05). PGPT’s [C 1<i>s</i>], [P 2<i>p</i>] and [Ca 2<i>p</i>] values were 24.3%, 5.6% and 11.0%, respectively, indicating that PGPT was an apatite without another crystalline phase. Cell viability and alkaline phosphatase assays revealed enhanced proliferation and osteoblastic differentiation for the cells cultivated in the PGPT media after 5 days (<i>p</i> < 0.05). The cells cultured in PGPT medium had higher bone sialoprotein and osteocalcin relative mRNA expression compared to cells cultured in non-treated porcine grafts (<i>p</i> < 0.05). CAP treatment of porcine particles did not modify the biomaterial’s surface and improved the proliferation and differentiation of osteoblast-like cells.https://www.mdpi.com/2079-6412/9/2/134argon plasma treatmentporcine bone graftbiological apatitechemical propertiesin-vitro behavior |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheuk Sing Choy Eisner Salamanca Pei Ying Lin Haw-Ming Huang Nai-Chia Teng Yu-Hwa Pan Wei-Jen Chang |
spellingShingle |
Cheuk Sing Choy Eisner Salamanca Pei Ying Lin Haw-Ming Huang Nai-Chia Teng Yu-Hwa Pan Wei-Jen Chang Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior Coatings argon plasma treatment porcine bone graft biological apatite chemical properties in-vitro behavior |
author_facet |
Cheuk Sing Choy Eisner Salamanca Pei Ying Lin Haw-Ming Huang Nai-Chia Teng Yu-Hwa Pan Wei-Jen Chang |
author_sort |
Cheuk Sing Choy |
title |
Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior |
title_short |
Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior |
title_full |
Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior |
title_fullStr |
Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior |
title_full_unstemmed |
Argon Plasma Surface Modified Porcine Bone Substitute Improved Osteoblast-Like Cell Behavior |
title_sort |
argon plasma surface modified porcine bone substitute improved osteoblast-like cell behavior |
publisher |
MDPI AG |
series |
Coatings |
issn |
2079-6412 |
publishDate |
2019-02-01 |
description |
Low-temperature plasma-treated porcine grafts (PGPT) may be an effective means for treating demanding osseous defects and enhance our understanding of plasma-tissue engineering. We chemically characterized porcine grafts under low-temperature Argon plasma treatment (CAP) and evaluated their biocompatibility in-vitro. Our results showed that PGPT did not differ in roughness, dominant crystalline phases, absorption peaks corresponding to phosphate band peaks, or micro-meso pore size, compared to non-treated porcine grafts. The PGPT Ca/P ratio was 2.16; whereas the porcine control ratio was 2.04 (<i>p</i> < 0.05). PGPT’s [C 1<i>s</i>], [P 2<i>p</i>] and [Ca 2<i>p</i>] values were 24.3%, 5.6% and 11.0%, respectively, indicating that PGPT was an apatite without another crystalline phase. Cell viability and alkaline phosphatase assays revealed enhanced proliferation and osteoblastic differentiation for the cells cultivated in the PGPT media after 5 days (<i>p</i> < 0.05). The cells cultured in PGPT medium had higher bone sialoprotein and osteocalcin relative mRNA expression compared to cells cultured in non-treated porcine grafts (<i>p</i> < 0.05). CAP treatment of porcine particles did not modify the biomaterial’s surface and improved the proliferation and differentiation of osteoblast-like cells. |
topic |
argon plasma treatment porcine bone graft biological apatite chemical properties in-vitro behavior |
url |
https://www.mdpi.com/2079-6412/9/2/134 |
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