Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae
The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase produ...
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Sociedade Brasileira de Microbiologia
2013-09-01
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doaj-59ea2073afc444a8ac94fbb5833e923d2020-11-24T23:43:18ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052013-09-0144384985410.1590/S1517-83822013000300028S1517-83822013000300028Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniaeMaryam Mobarak-Qamsari0Mitra Ashayeri-Panah1Freshteh Eftekhar2Mohammad Mehdi Feizabadi3Shahid Beheshti UniversityShahid Beheshti UniversityShahid Beheshti UniversityTehran University of Medical SciencesThe present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined to 17 antibiotics by disc diffusion. Presence of integron classes 1, 2 and 3 was detected by PCR and integrase specific primers. Isolates harboring class 1 integron were then screened for variable regions using PCR. Fifty isolates (48%) produced extended-spectrum β-lactamases among which, 22 (44%) harbored class 1, 3 (6%) carried class 2 and none contained class 3 integons. Integron carriage was significantly associated with higher rates of multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of K. pneumoniae. Integron harboring isolates were more resistant to aztreonam (51.3%), ceftazidime (42.6%), cefotaxime (43.3%), cefepime (24.6%), kanamycin (43.2%), tobramycin (30.7%), norfloxcacin (32%) and spectinomycin (25.6%) compared to the organisms without integrons. On the other hand, resistance to nitrofurantoin and streptomycin was significantly higher among the integron negative isolates. PCR amplification of class1 integron variable regions revealed 9 different sized DNA fragments and isolates with similar profiles for class 1 integron variable regions showed the same antibiotic resistance phenotypes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300028&lng=en&tlng=enKlebsiella pneumoniaeextended-spectrum β-lactamasemultiple antibiotic resistanceintegron |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maryam Mobarak-Qamsari Mitra Ashayeri-Panah Freshteh Eftekhar Mohammad Mehdi Feizabadi |
spellingShingle |
Maryam Mobarak-Qamsari Mitra Ashayeri-Panah Freshteh Eftekhar Mohammad Mehdi Feizabadi Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae Brazilian Journal of Microbiology Klebsiella pneumoniae extended-spectrum β-lactamase multiple antibiotic resistance integron |
author_facet |
Maryam Mobarak-Qamsari Mitra Ashayeri-Panah Freshteh Eftekhar Mohammad Mehdi Feizabadi |
author_sort |
Maryam Mobarak-Qamsari |
title |
Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae |
title_short |
Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae |
title_full |
Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae |
title_fullStr |
Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae |
title_full_unstemmed |
Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae |
title_sort |
integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of klebsiella pneumoniae |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2013-09-01 |
description |
The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined to 17 antibiotics by disc diffusion. Presence of integron classes 1, 2 and 3 was detected by PCR and integrase specific primers. Isolates harboring class 1 integron were then screened for variable regions using PCR. Fifty isolates (48%) produced extended-spectrum β-lactamases among which, 22 (44%) harbored class 1, 3 (6%) carried class 2 and none contained class 3 integons. Integron carriage was significantly associated with higher rates of multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of K. pneumoniae. Integron harboring isolates were more resistant to aztreonam (51.3%), ceftazidime (42.6%), cefotaxime (43.3%), cefepime (24.6%), kanamycin (43.2%), tobramycin (30.7%), norfloxcacin (32%) and spectinomycin (25.6%) compared to the organisms without integrons. On the other hand, resistance to nitrofurantoin and streptomycin was significantly higher among the integron negative isolates. PCR amplification of class1 integron variable regions revealed 9 different sized DNA fragments and isolates with similar profiles for class 1 integron variable regions showed the same antibiotic resistance phenotypes. |
topic |
Klebsiella pneumoniae extended-spectrum β-lactamase multiple antibiotic resistance integron |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300028&lng=en&tlng=en |
work_keys_str_mv |
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