Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007
<p>Abstract</p> <p>Background</p> <p>From October 2006 to February 2007, clinical specimens from 452 patients with symptoms related to respiratory tract infection in the northern region of Taiwan were collected. Real-time PCR and direct immunofluorescent antibody tests...
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doaj-59ec90dad08f41ef971ca4bd4a376d222020-11-25T02:01:55ZengBMCBMC Research Notes1756-05002009-05-01218610.1186/1756-0500-2-86Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007Chen Kuan-HsuanLee Cheng-MingWang Sheng-FanLee Yuan-MingChan Yu-JiunLiu Wu-TseChen Yi-Ming<p>Abstract</p> <p>Background</p> <p>From October 2006 to February 2007, clinical specimens from 452 patients with symptoms related to respiratory tract infection in the northern region of Taiwan were collected. Real-time PCR and direct immunofluorescent antibody tests showed that 145 (32%) patients had influenza B virus infections. Subsequently, nucleotide sequence analyses of both hemagglutinin (HA) and neuraminidase (NA) genes of 39 isolates were performed. Isolated viruses were antigenically characterized using hemagglutinin inhibition (HI) test.</p> <p>Findings</p> <p>Phylogenetic tree analysis showed that all the isolates belonged to the B reassortant lineage with HA gene belonged to the B/Victoria/2/87 lineage and the NA gene belonged to the B/Yamagata/16/88 lineage. In addition, a group of children aged between 6 to 8 years old resided in Yilan county were infected with a variant strain. Hemagglutinin inhibition (HI) tests confirmed that all the reassortant influenza B viruses were B/Malaysia/2506/04-like viruses. Pre- and post-immunized serum samples from 4 normal volunteers inoculated with 2007 influenza vaccine were evaluated for their HI activity on 6 reassortant B isolates including two variants that we found in the Yilan county. The results demonstrated that after vaccination, all four vaccinees had at least 4-fold increases of their HI titers.</p> <p>Conclusion</p> <p>The results indicate that the 2006–2007 seasonal influenza vaccine was effective in stimulating protective immunity against the influenza B variants identified in Yilan county. Continuous surveillance of emerging influenza B variants in the northern region of Taiwan is important for the selection of proper vaccine candidate in the future.</p> http://www.biomedcentral.com/1756-0500/2/86 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chen Kuan-Hsuan Lee Cheng-Ming Wang Sheng-Fan Lee Yuan-Ming Chan Yu-Jiun Liu Wu-Tse Chen Yi-Ming |
spellingShingle |
Chen Kuan-Hsuan Lee Cheng-Ming Wang Sheng-Fan Lee Yuan-Ming Chan Yu-Jiun Liu Wu-Tse Chen Yi-Ming Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 BMC Research Notes |
author_facet |
Chen Kuan-Hsuan Lee Cheng-Ming Wang Sheng-Fan Lee Yuan-Ming Chan Yu-Jiun Liu Wu-Tse Chen Yi-Ming |
author_sort |
Chen Kuan-Hsuan |
title |
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 |
title_short |
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 |
title_full |
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 |
title_fullStr |
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 |
title_full_unstemmed |
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007 |
title_sort |
virological investigation of four outbreaks of influenza b reassortants in the northern region of taiwan from october 2006 to february 2007 |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2009-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p>From October 2006 to February 2007, clinical specimens from 452 patients with symptoms related to respiratory tract infection in the northern region of Taiwan were collected. Real-time PCR and direct immunofluorescent antibody tests showed that 145 (32%) patients had influenza B virus infections. Subsequently, nucleotide sequence analyses of both hemagglutinin (HA) and neuraminidase (NA) genes of 39 isolates were performed. Isolated viruses were antigenically characterized using hemagglutinin inhibition (HI) test.</p> <p>Findings</p> <p>Phylogenetic tree analysis showed that all the isolates belonged to the B reassortant lineage with HA gene belonged to the B/Victoria/2/87 lineage and the NA gene belonged to the B/Yamagata/16/88 lineage. In addition, a group of children aged between 6 to 8 years old resided in Yilan county were infected with a variant strain. Hemagglutinin inhibition (HI) tests confirmed that all the reassortant influenza B viruses were B/Malaysia/2506/04-like viruses. Pre- and post-immunized serum samples from 4 normal volunteers inoculated with 2007 influenza vaccine were evaluated for their HI activity on 6 reassortant B isolates including two variants that we found in the Yilan county. The results demonstrated that after vaccination, all four vaccinees had at least 4-fold increases of their HI titers.</p> <p>Conclusion</p> <p>The results indicate that the 2006–2007 seasonal influenza vaccine was effective in stimulating protective immunity against the influenza B variants identified in Yilan county. Continuous surveillance of emerging influenza B variants in the northern region of Taiwan is important for the selection of proper vaccine candidate in the future.</p> |
url |
http://www.biomedcentral.com/1756-0500/2/86 |
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