Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate
Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment...
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doaj-59f7168690ff47dba3ba93c62530ed142020-12-31T00:01:13ZengMDPI AGApplied Sciences2076-34172021-12-011127527510.3390/app11010275Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz SubstrateJan Slavík0Vratislav Čmiel1Jaromír Hubálek2Yi Yang3Tian-Ling Ren4Institute of Microelectronics & Beijing National Research Center for Information Science and Technology (BNRist), Tsinghua University, Beijing 100084, ChinaDepartment of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 3082/12, 61600 Brno, Czech RepublicCentral European Institute of Technology, Brno University of Technology, Purkyňova 123, 61600 Brno, Czech RepublicInstitute of Microelectronics & Beijing National Research Center for Information Science and Technology (BNRist), Tsinghua University, Beijing 100084, ChinaInstitute of Microelectronics & Beijing National Research Center for Information Science and Technology (BNRist), Tsinghua University, Beijing 100084, ChinaAlignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves.https://www.mdpi.com/2076-3417/11/1/275hippocampal neuronscell alignmentcell patterningquartz groovesparylene cues |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Slavík Vratislav Čmiel Jaromír Hubálek Yi Yang Tian-Ling Ren |
spellingShingle |
Jan Slavík Vratislav Čmiel Jaromír Hubálek Yi Yang Tian-Ling Ren Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate Applied Sciences hippocampal neurons cell alignment cell patterning quartz grooves parylene cues |
author_facet |
Jan Slavík Vratislav Čmiel Jaromír Hubálek Yi Yang Tian-Ling Ren |
author_sort |
Jan Slavík |
title |
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate |
title_short |
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate |
title_full |
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate |
title_fullStr |
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate |
title_full_unstemmed |
Hippocampal Neurons’ Alignment on Quartz Grooves and Parylene Cues on Quartz Substrate |
title_sort |
hippocampal neurons’ alignment on quartz grooves and parylene cues on quartz substrate |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-12-01 |
description |
Alignment and patterning of neurons have great importance in some research fields, especially for regenerative medicine and for the formation of artificial neural networks. Alignment of neurons on quartz grooves and parylene cues on quartz substrate was evaluated in this work. The neurons’ alignment on quartz grooves is considered to be topographical alignment, while the neurons’ alignment on parylene cues on quartz substrate is considered to be chemical alignment. Both quartz grooves’ and parylene cues’ widths were fabricated in a range from 2 µm to 8 µm; quartz grooves’ heights were in a range from 0.25 µm to 4 µm, while parylene cues’ heights were only 0.25 µm. Neurons were dissociated hippocampal neurons from rat E18. Neurons were cultivated on test substrates for 7 days before alignment evaluation. As expected, neurons aligned according to the direction of grooves and cues; however, transversal growth direction was also observed with much smaller tendency. Chemical alignment was found to be more effective than topographical alignment. If parylene cues are thin and distanced enough, then neurons have a tendency to follow the direction of individual parylene cues; however, neurons on quartz grooves have a tendency just to follow a preferable direction than individual quartz grooves. |
topic |
hippocampal neurons cell alignment cell patterning quartz grooves parylene cues |
url |
https://www.mdpi.com/2076-3417/11/1/275 |
work_keys_str_mv |
AT janslavik hippocampalneuronsalignmentonquartzgroovesandparylenecuesonquartzsubstrate AT vratislavcmiel hippocampalneuronsalignmentonquartzgroovesandparylenecuesonquartzsubstrate AT jaromirhubalek hippocampalneuronsalignmentonquartzgroovesandparylenecuesonquartzsubstrate AT yiyang hippocampalneuronsalignmentonquartzgroovesandparylenecuesonquartzsubstrate AT tianlingren hippocampalneuronsalignmentonquartzgroovesandparylenecuesonquartzsubstrate |
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