Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond
Waterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study...
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doaj-59fbbbac4e654577a7c9c0f6970df4882020-11-25T00:13:43ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-05-01196157610.3390/ijms19061576ijms19061576Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor PondAida Rasyidah Azman0Naji Arafat Mahat1Roswanira Abdul Wahab2Fazira Ilyana Abdul Razak3Hafezul Helmi Hamzah4Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, MalaysiaCriminal Investigation Department, Criminal Intelligence (D4), Royal Malaysia Police, Kuala Lumpur 50560, MalaysiaWaterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study proposes an eco-friendly, safranin-tinted Candida rugosa lipase (triacylglycerol ester hydrolysis EC 3.1.1.3) with functionalized carbon nanotubes (CRL-MWCNTS/GA/SAF) as an alternative reagent to the small particle reagent. The CRL-MWCNTS/GA/SAF reagent was compared with the small particle reagent to visualize groomed, full fingerprints deposited on stainless steel knives which were immersed in a natural outdoor pond for 30 days. The quality of visualized fingerprints using the new reagent was similar (modified-Centre for Applied Science and Technology grade: 4; p > 0.05) to small particle reagent, even after 15 days of immersion. Despite the slight decrease in quality of visualized fingerprints using the CRL-MWCNTS/GA/SAF on the last three immersion periods, the fingerprints remained forensically identifiable (modified-Centre for Applied Science and Technology grade: 3). The possible chemical interactions that enabled successful visualization is also discussed. Thus, this novel reagent may provide a relatively greener alternative for the visualization of latent fingerprints on immersed non-porous objects.http://www.mdpi.com/1422-0067/19/6/1576forensic sciencelatent fingerprintsnon-porous objectsimmersionnatural outdoor pondCandida rugosa lipase nanoconjugatesnanobio-based method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aida Rasyidah Azman Naji Arafat Mahat Roswanira Abdul Wahab Fazira Ilyana Abdul Razak Hafezul Helmi Hamzah |
spellingShingle |
Aida Rasyidah Azman Naji Arafat Mahat Roswanira Abdul Wahab Fazira Ilyana Abdul Razak Hafezul Helmi Hamzah Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond International Journal of Molecular Sciences forensic science latent fingerprints non-porous objects immersion natural outdoor pond Candida rugosa lipase nanoconjugates nanobio-based method |
author_facet |
Aida Rasyidah Azman Naji Arafat Mahat Roswanira Abdul Wahab Fazira Ilyana Abdul Razak Hafezul Helmi Hamzah |
author_sort |
Aida Rasyidah Azman |
title |
Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond |
title_short |
Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond |
title_full |
Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond |
title_fullStr |
Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond |
title_full_unstemmed |
Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond |
title_sort |
novel safranin-tinted candida rugosa lipase nanoconjugates reagent for visualizing latent fingerprints on stainless steel knives immersed in a natural outdoor pond |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-05-01 |
description |
Waterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study proposes an eco-friendly, safranin-tinted Candida rugosa lipase (triacylglycerol ester hydrolysis EC 3.1.1.3) with functionalized carbon nanotubes (CRL-MWCNTS/GA/SAF) as an alternative reagent to the small particle reagent. The CRL-MWCNTS/GA/SAF reagent was compared with the small particle reagent to visualize groomed, full fingerprints deposited on stainless steel knives which were immersed in a natural outdoor pond for 30 days. The quality of visualized fingerprints using the new reagent was similar (modified-Centre for Applied Science and Technology grade: 4; p > 0.05) to small particle reagent, even after 15 days of immersion. Despite the slight decrease in quality of visualized fingerprints using the CRL-MWCNTS/GA/SAF on the last three immersion periods, the fingerprints remained forensically identifiable (modified-Centre for Applied Science and Technology grade: 3). The possible chemical interactions that enabled successful visualization is also discussed. Thus, this novel reagent may provide a relatively greener alternative for the visualization of latent fingerprints on immersed non-porous objects. |
topic |
forensic science latent fingerprints non-porous objects immersion natural outdoor pond Candida rugosa lipase nanoconjugates nanobio-based method |
url |
http://www.mdpi.com/1422-0067/19/6/1576 |
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