Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells
In general, neurodegenerative disorders have a great deal of correlation with the misfolded as well as aggregated forms of protein-based macromolecules. Among various species formed during the aggregation process, protein oligomers have been classified as most toxic entities against several types of...
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doaj-5a0049c60ed948388eaa3f2791664fe12020-11-24T21:24:07ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-11-01710.3389/fchem.2019.00787493482Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma CellsAnzar Abdul Mujeeb0Khan Farheen Badre Alam1Ansam Wadia Faid Alshameri2Fauzia Jamal3Saba Farheen4Mohd Kashif5Anees Ahmed6Irfan Ahmad Ghazi7Mohammad Owais8Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaCSIR-National Botanical Research Institute, Lucknow, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaIn general, neurodegenerative disorders have a great deal of correlation with the misfolded as well as aggregated forms of protein-based macromolecules. Among various species formed during the aggregation process, protein oligomers have been classified as most toxic entities against several types of living cells. A series of chemicals have been developed to inhibit protein aggregation as a measure to regulate neurodegenerative diseases. Recently, various classes of nanoparticles have also been reported to inhibit protein aggregation. In the present study, we synthesized fluorescent gold nanoparticles (B-AuNPs) employing Olax scandens leaf extract. Next, an in vitro study was performed to assess the effect of as-synthesized B-AuNPs on the aggregation behavior of the ovalbumin (OVA) and other related model proteins. We performed an extensive study to elucidate anti-amyloidogenic properties of nano-sized entities and established that small-sized B-AuNPs manifest chaperone potential against protein aggregation. Further, we exploited as-synthesized B-AuNPs as a mean to prevent protein aggregation mediated toxicity in neuroblastoma cells.https://www.frontiersin.org/article/10.3389/fchem.2019.00787/fullgold nanoparticlesbiogenic nanoparticleschaperoneamyloidfibril |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anzar Abdul Mujeeb Khan Farheen Badre Alam Ansam Wadia Faid Alshameri Fauzia Jamal Saba Farheen Mohd Kashif Anees Ahmed Irfan Ahmad Ghazi Mohammad Owais |
spellingShingle |
Anzar Abdul Mujeeb Khan Farheen Badre Alam Ansam Wadia Faid Alshameri Fauzia Jamal Saba Farheen Mohd Kashif Anees Ahmed Irfan Ahmad Ghazi Mohammad Owais Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells Frontiers in Chemistry gold nanoparticles biogenic nanoparticles chaperone amyloid fibril |
author_facet |
Anzar Abdul Mujeeb Khan Farheen Badre Alam Ansam Wadia Faid Alshameri Fauzia Jamal Saba Farheen Mohd Kashif Anees Ahmed Irfan Ahmad Ghazi Mohammad Owais |
author_sort |
Anzar Abdul Mujeeb |
title |
Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells |
title_short |
Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells |
title_full |
Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells |
title_fullStr |
Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells |
title_full_unstemmed |
Chaperone Like Attributes of Biogenic Fluorescent Gold Nanoparticles: Potential to Alleviate Toxicity Induced by Intermediate State Fibrils Against Neuroblastoma Cells |
title_sort |
chaperone like attributes of biogenic fluorescent gold nanoparticles: potential to alleviate toxicity induced by intermediate state fibrils against neuroblastoma cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2019-11-01 |
description |
In general, neurodegenerative disorders have a great deal of correlation with the misfolded as well as aggregated forms of protein-based macromolecules. Among various species formed during the aggregation process, protein oligomers have been classified as most toxic entities against several types of living cells. A series of chemicals have been developed to inhibit protein aggregation as a measure to regulate neurodegenerative diseases. Recently, various classes of nanoparticles have also been reported to inhibit protein aggregation. In the present study, we synthesized fluorescent gold nanoparticles (B-AuNPs) employing Olax scandens leaf extract. Next, an in vitro study was performed to assess the effect of as-synthesized B-AuNPs on the aggregation behavior of the ovalbumin (OVA) and other related model proteins. We performed an extensive study to elucidate anti-amyloidogenic properties of nano-sized entities and established that small-sized B-AuNPs manifest chaperone potential against protein aggregation. Further, we exploited as-synthesized B-AuNPs as a mean to prevent protein aggregation mediated toxicity in neuroblastoma cells. |
topic |
gold nanoparticles biogenic nanoparticles chaperone amyloid fibril |
url |
https://www.frontiersin.org/article/10.3389/fchem.2019.00787/full |
work_keys_str_mv |
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