The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

Abstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV c...

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Main Authors:	Emma L. Hesketh, Yulia Meshcheriakova, Rebecca F. Thompson, George P. Lomonossoff, Neil A. Ranson
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-04-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-017-00533-w

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spelling	doaj-5a08a9447f2247ffa6187b87a1890e272020-12-08T01:05:41ZengNature Publishing GroupScientific Reports2045-23222017-04-017111010.1038/s41598-017-00533-wThe structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopyEmma L. Hesketh0Yulia Meshcheriakova1Rebecca F. Thompson2George P. Lomonossoff3Neil A. Ranson4Astbury Centre for Structural Molecular Biology, University of LeedsDepartment of Biological Chemistry, John Innes Centre, Norwich Research ParkAstbury Centre for Structural Molecular Biology, University of LeedsDepartment of Biological Chemistry, John Innes Centre, Norwich Research ParkAstbury Centre for Structural Molecular Biology, University of LeedsAbstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.https://doi.org/10.1038/s41598-017-00533-w
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson
spellingShingle	Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy Scientific Reports
author_facet	Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson
author_sort	Emma L. Hesketh
title	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_short	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_full	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_fullStr	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_full_unstemmed	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_sort	structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2017-04-01
description	Abstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.
url	https://doi.org/10.1038/s41598-017-00533-w
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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

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