Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration
Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa. The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated...
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doaj-5a108c0cc3dd4cd098fe5ec2de79498e2020-11-24T20:50:58ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292014-12-01810.3389/fnana.2014.00151120244Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degenerationNicolás eCuenca0Nicolás eCuenca1Laura eFernandez-Sanchez2Yves eSauvé3Francisco Javier Segura4Francisco Javier Segura5Gema Concepcion Martínez-Navarrete6Jose Manuel Tamarit7Lorena eFuentes-Broto8Lorena eFuentes-Broto9Ana eSanchez-Cano10Ana eSanchez-Cano11Isabel ePinilla12Isabel ePinilla13Isabel ePinilla14University of AlicanteMultidisciplinary Institute for Environmental Studies Ramon MargalefUniversity of AlicanteUniversity of AlbertaAragon Health Science Institute. IIS Aragon.School of Medicine. University of ZaragozaMiguel Hernandez UniversityBloss Group Company. Spain and Heidelberg Engineering Gmbh.Aragon Health Science Institute. IIS Aragon.University of ZaragozaAragon Health Science Institute. IIS Aragon.University of ZaragozaAragon Health Science Institute. IIS Aragon.School of Medicine. University of ZaragozaLozano Blesa University Hospital.Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa. The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer, and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration.http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00151/fullImmunohistochemistryRetinal DegenerationRetinitis PigmentosaVisual AcuityElectroretinogramOptical Coherence Tomography |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicolás eCuenca Nicolás eCuenca Laura eFernandez-Sanchez Yves eSauvé Francisco Javier Segura Francisco Javier Segura Gema Concepcion Martínez-Navarrete Jose Manuel Tamarit Lorena eFuentes-Broto Lorena eFuentes-Broto Ana eSanchez-Cano Ana eSanchez-Cano Isabel ePinilla Isabel ePinilla Isabel ePinilla |
spellingShingle |
Nicolás eCuenca Nicolás eCuenca Laura eFernandez-Sanchez Yves eSauvé Francisco Javier Segura Francisco Javier Segura Gema Concepcion Martínez-Navarrete Jose Manuel Tamarit Lorena eFuentes-Broto Lorena eFuentes-Broto Ana eSanchez-Cano Ana eSanchez-Cano Isabel ePinilla Isabel ePinilla Isabel ePinilla Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration Frontiers in Neuroanatomy Immunohistochemistry Retinal Degeneration Retinitis Pigmentosa Visual Acuity Electroretinogram Optical Coherence Tomography |
author_facet |
Nicolás eCuenca Nicolás eCuenca Laura eFernandez-Sanchez Yves eSauvé Francisco Javier Segura Francisco Javier Segura Gema Concepcion Martínez-Navarrete Jose Manuel Tamarit Lorena eFuentes-Broto Lorena eFuentes-Broto Ana eSanchez-Cano Ana eSanchez-Cano Isabel ePinilla Isabel ePinilla Isabel ePinilla |
author_sort |
Nicolás eCuenca |
title |
Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
title_short |
Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
title_full |
Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
title_fullStr |
Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
title_full_unstemmed |
Correlation between SD-OCT, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
title_sort |
correlation between sd-oct, immunocytochemistry and functional findings in a pigmented animal model of retinal degeneration |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroanatomy |
issn |
1662-5129 |
publishDate |
2014-12-01 |
description |
Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa. The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer, and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration. |
topic |
Immunohistochemistry Retinal Degeneration Retinitis Pigmentosa Visual Acuity Electroretinogram Optical Coherence Tomography |
url |
http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00151/full |
work_keys_str_mv |
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