Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1
An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradi...
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2008-12-01
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Online Access: | http://www.mdpi.com/1424-8220/8/12/8262/ |
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doaj-5a535f1c82a54f3d87519b133f99ce802020-11-25T00:55:04ZengMDPI AGSensors1424-82202008-12-018128262827410.3390/s8128262Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1Joseph H.O. OwinoNicolette HendricksOmotayo A. ArotibaEverlyne A. SongaNazeem JahedTesfaye T. WaryoEmmanuel I. IwuohaPriscilla G .L. BakerRachel F. NgeceAn aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor’s differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA–based immunosensors.http://www.mdpi.com/1424-8220/8/12/8262/ImmunosensorGold nanoparticlesAflatoxin B1PolythionineHorseradish peroxidise (HRP) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joseph H.O. Owino Nicolette Hendricks Omotayo A. Arotiba Everlyne A. Songa Nazeem Jahed Tesfaye T. Waryo Emmanuel I. Iwuoha Priscilla G .L. Baker Rachel F. Ngece |
spellingShingle |
Joseph H.O. Owino Nicolette Hendricks Omotayo A. Arotiba Everlyne A. Songa Nazeem Jahed Tesfaye T. Waryo Emmanuel I. Iwuoha Priscilla G .L. Baker Rachel F. Ngece Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 Sensors Immunosensor Gold nanoparticles Aflatoxin B1 Polythionine Horseradish peroxidise (HRP) |
author_facet |
Joseph H.O. Owino Nicolette Hendricks Omotayo A. Arotiba Everlyne A. Songa Nazeem Jahed Tesfaye T. Waryo Emmanuel I. Iwuoha Priscilla G .L. Baker Rachel F. Ngece |
author_sort |
Joseph H.O. Owino |
title |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
title_short |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
title_full |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
title_fullStr |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
title_full_unstemmed |
Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1 |
title_sort |
electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of aflatoxin b1 |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2008-12-01 |
description |
An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor’s differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA–based immunosensors. |
topic |
Immunosensor Gold nanoparticles Aflatoxin B1 Polythionine Horseradish peroxidise (HRP) |
url |
http://www.mdpi.com/1424-8220/8/12/8262/ |
work_keys_str_mv |
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