Preparation of β(1→3)/β(1→4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus

• Main Authors: Yuki Fujii, Manami Kobayashi, Yoshikatsu Miyabe, Hideki Kishimura, Tadashi Hatanaka, Yuya Kumagai
• Format: Article
• Language: English
• Published: SpringerOpen 2021-05-01
• Series: Bioresources and Bioprocessing
• Subjects: Palmaria palmata in Japan; β(1→3)/β(1→4)-xylooligosaccharides production; GH10 and GH11 endoxylanase; Actinomycete
• Online Access: https://doi.org/10.1186/s40643-021-00390-6

Abstract Red alga dulse contains xylan with β(1→3)/β(1→4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many d-xylose residues and fewer XOSs with β(1→3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k cat/K m values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 μg/mL StXyl10 for 4 h at 50 °C and then 2.0 μg/mL StXyl11 for 36 h at 60 °C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a β(1→3) linkage and 0.97% d-xylose. Our study provides useful information for the production of XOSs with β(1→3) linkages.