Ameliorative effects of colostrum against DMBA hepatotoxicity in rats
Colostrum, the sole diet for newborns, is an emerging nutraceutical. To date, the chemopreventive effect of Bovine Colostrum against liver injury induced by the potent carcinogen, 7,12-dimethyl-Benz[a]anthracene (DMBA) is unexplored. Humans are daily exposed to DMBA which is a highly lipophilic envi...
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doaj-5aa54d9b284c40708cd21c5acdeefe492021-04-04T04:18:03ZengElsevierSaudi Journal of Biological Sciences1319-562X2021-04-0128422542266Ameliorative effects of colostrum against DMBA hepatotoxicity in ratsNabila E. Abdelmeguid0Mahmoud I. Khalil1Nada S. Badr2Afrah F. Alkhuriji3Mohamed S.A. El-Gerbed4Ahmed S. Sultan5Zoology Department, Faculty of Science, Alexandria University, Alexandria, EgyptDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon; Molecular Biology Unit, Zoology Department, Faculty of Science, Alexandria University, Alexandria, EgyptZoology Department, Faculty of Science, Damanhur University, Damanhur, EgyptDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaZoology Department, Faculty of Science, Damanhur University, Damanhur, Egypt; Corresponding author.Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, EgyptColostrum, the sole diet for newborns, is an emerging nutraceutical. To date, the chemopreventive effect of Bovine Colostrum against liver injury induced by the potent carcinogen, 7,12-dimethyl-Benz[a]anthracene (DMBA) is unexplored. Humans are daily exposed to DMBA which is a highly lipophilic environmental organic pollutant. The study aimed to investigate the hepatoprotective role of Bovine Colostrum against DMBA-induced hepatotoxicity using a rat model. Fifty male rats were divided into five groups; GI (control), GII (olive oil, vehicle for DMBA), GIII (DMBA), GIV (DMBA + Bovine Colostrum), GV (Bovine Colostrum). After 12 weeks, body weight changes and mortality were calculated. Histological and ultrastructural examinations of liver tissue were performed. Expressions of p53, TGFβ2, TNF-α, S6K2, and c20orf20 were assessed by RT-PCR. Post-treatment with Bovine Colostrum increased both the body weight and the survival rate of rats treated with DMBA. In addition, remarkable protection against the pathological effect of DMBA was noted. Ultrastructurally, Bovine Colostrum ameliorated/prevented most of the toxic effects of DMBA on hepatocytes, including irregularities of nuclear envelope, clumping, and margination of heterochromatin aggregates, segregated nucleoli, and mitochondrial pleomorphism. Bovine Colostrum administration down-regulated p53, C20orf20, and S6K2 mRNA levels, and up-regulated TNF-α and TGFβ2. In conclusion, Bovine Colostrum have a protective effect against DMBA-induced toxicity on the liver of albino rats. Consequently, Bovine Colostrum may prevent polycyclic aromatic hydrocarbons-induced hepatotoxicity and may be useful in promoting human health if supplemented in the diet.http://www.sciencedirect.com/science/article/pii/S1319562X21000164ColostrumNutraceuticalDMBAHepatoprotectiveS6K2p53 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nabila E. Abdelmeguid Mahmoud I. Khalil Nada S. Badr Afrah F. Alkhuriji Mohamed S.A. El-Gerbed Ahmed S. Sultan |
spellingShingle |
Nabila E. Abdelmeguid Mahmoud I. Khalil Nada S. Badr Afrah F. Alkhuriji Mohamed S.A. El-Gerbed Ahmed S. Sultan Ameliorative effects of colostrum against DMBA hepatotoxicity in rats Saudi Journal of Biological Sciences Colostrum Nutraceutical DMBA Hepatoprotective S6K2 p53 |
author_facet |
Nabila E. Abdelmeguid Mahmoud I. Khalil Nada S. Badr Afrah F. Alkhuriji Mohamed S.A. El-Gerbed Ahmed S. Sultan |
author_sort |
Nabila E. Abdelmeguid |
title |
Ameliorative effects of colostrum against DMBA hepatotoxicity in rats |
title_short |
Ameliorative effects of colostrum against DMBA hepatotoxicity in rats |
title_full |
Ameliorative effects of colostrum against DMBA hepatotoxicity in rats |
title_fullStr |
Ameliorative effects of colostrum against DMBA hepatotoxicity in rats |
title_full_unstemmed |
Ameliorative effects of colostrum against DMBA hepatotoxicity in rats |
title_sort |
ameliorative effects of colostrum against dmba hepatotoxicity in rats |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2021-04-01 |
description |
Colostrum, the sole diet for newborns, is an emerging nutraceutical. To date, the chemopreventive effect of Bovine Colostrum against liver injury induced by the potent carcinogen, 7,12-dimethyl-Benz[a]anthracene (DMBA) is unexplored. Humans are daily exposed to DMBA which is a highly lipophilic environmental organic pollutant. The study aimed to investigate the hepatoprotective role of Bovine Colostrum against DMBA-induced hepatotoxicity using a rat model. Fifty male rats were divided into five groups; GI (control), GII (olive oil, vehicle for DMBA), GIII (DMBA), GIV (DMBA + Bovine Colostrum), GV (Bovine Colostrum). After 12 weeks, body weight changes and mortality were calculated. Histological and ultrastructural examinations of liver tissue were performed. Expressions of p53, TGFβ2, TNF-α, S6K2, and c20orf20 were assessed by RT-PCR. Post-treatment with Bovine Colostrum increased both the body weight and the survival rate of rats treated with DMBA. In addition, remarkable protection against the pathological effect of DMBA was noted. Ultrastructurally, Bovine Colostrum ameliorated/prevented most of the toxic effects of DMBA on hepatocytes, including irregularities of nuclear envelope, clumping, and margination of heterochromatin aggregates, segregated nucleoli, and mitochondrial pleomorphism. Bovine Colostrum administration down-regulated p53, C20orf20, and S6K2 mRNA levels, and up-regulated TNF-α and TGFβ2. In conclusion, Bovine Colostrum have a protective effect against DMBA-induced toxicity on the liver of albino rats. Consequently, Bovine Colostrum may prevent polycyclic aromatic hydrocarbons-induced hepatotoxicity and may be useful in promoting human health if supplemented in the diet. |
topic |
Colostrum Nutraceutical DMBA Hepatoprotective S6K2 p53 |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X21000164 |
work_keys_str_mv |
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