Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty
Fu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In...
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doaj-5ab5647c3f6d4a2f8f5ae73b76a75f612021-05-07T09:24:23ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-05-011210.3389/fphar.2021.649732649732Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious PubertyChunyan Guo0Ning Sun1Kaili Hu2Guoliang Bai3Meng Zhang4Qian Wang5Qian Ding6Jing Liu7Xiaoling Wang8Libo Zhao9Department of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaSchool of Traditional Chinese Medicine Department, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Traditional Chinese medicine, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaDepartment of Pharmacy, National Center for Children Health, Beijing Children’s Hospital, Capital Medical University, Beijing, ChinaFu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In this study, we analyze the components and mechanisms of FY in PP, based on the integrated pharmacology. Investigated main constituents, targets, pathways of FY by using an integrative pharmacology, and recognized main constituents by HPLC-MS/MS. Then, observed the levels of Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (E2) in danazol-induced PP in Sprague–Dawley (SD) rats. Lastly, retrospective study analyzed the clinical data of 575 patients who were diagnosed PP, treated by the FY, and followed-up in our hospital from 2014–2020. The result that total of 116 important candidate targets were selected based on pharmacological analysis. Selected the top 10 values key targets such as the estrogen receptor alpha (ESR1) and insulin-like growth factor 1 (IGF1), were localized and the related gene functions were determined. Gene functions were associated with biological regulation, a cellular process, or signaling pathway, such as the Estrogen signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway. By recognizing the five compounds related to the ESR1 and IGF1, which are Quercetin, kaempferol, Luteolin, Apigenin, and Emodin. The results of the molecular docking study further showed that the flavonoids had a strong binding affinity for ESR1 and IGF1 after docking into the crystal structure. The results showed that the FY could effectively reduce E2, LH, and FSH levels in SD rats. Furthermore, the results of the retrospective analysis of medical records showed that the FY could remarkably reduce E2 levels in girls with PP.https://www.frontiersin.org/articles/10.3389/fphar.2021.649732/fullintegrated pharmacologyprecocious pubertyChinese medicineprotein-protein interaction networkFuyou formula |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunyan Guo Ning Sun Kaili Hu Guoliang Bai Meng Zhang Qian Wang Qian Ding Jing Liu Xiaoling Wang Libo Zhao |
spellingShingle |
Chunyan Guo Ning Sun Kaili Hu Guoliang Bai Meng Zhang Qian Wang Qian Ding Jing Liu Xiaoling Wang Libo Zhao Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty Frontiers in Pharmacology integrated pharmacology precocious puberty Chinese medicine protein-protein interaction network Fuyou formula |
author_facet |
Chunyan Guo Ning Sun Kaili Hu Guoliang Bai Meng Zhang Qian Wang Qian Ding Jing Liu Xiaoling Wang Libo Zhao |
author_sort |
Chunyan Guo |
title |
Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty |
title_short |
Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty |
title_full |
Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty |
title_fullStr |
Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty |
title_full_unstemmed |
Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty |
title_sort |
integrated pharmacological analysis on the mechanism of fuyou formula in treating precocious puberty |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-05-01 |
description |
Fu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In this study, we analyze the components and mechanisms of FY in PP, based on the integrated pharmacology. Investigated main constituents, targets, pathways of FY by using an integrative pharmacology, and recognized main constituents by HPLC-MS/MS. Then, observed the levels of Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (E2) in danazol-induced PP in Sprague–Dawley (SD) rats. Lastly, retrospective study analyzed the clinical data of 575 patients who were diagnosed PP, treated by the FY, and followed-up in our hospital from 2014–2020. The result that total of 116 important candidate targets were selected based on pharmacological analysis. Selected the top 10 values key targets such as the estrogen receptor alpha (ESR1) and insulin-like growth factor 1 (IGF1), were localized and the related gene functions were determined. Gene functions were associated with biological regulation, a cellular process, or signaling pathway, such as the Estrogen signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway. By recognizing the five compounds related to the ESR1 and IGF1, which are Quercetin, kaempferol, Luteolin, Apigenin, and Emodin. The results of the molecular docking study further showed that the flavonoids had a strong binding affinity for ESR1 and IGF1 after docking into the crystal structure. The results showed that the FY could effectively reduce E2, LH, and FSH levels in SD rats. Furthermore, the results of the retrospective analysis of medical records showed that the FY could remarkably reduce E2 levels in girls with PP. |
topic |
integrated pharmacology precocious puberty Chinese medicine protein-protein interaction network Fuyou formula |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.649732/full |
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