Bone marrow characterization in COPD: a multi-level network analysis
Abstract Background Bone marrow (BM) produces hematopoietic and progenitor cells that contribute to distant organ inflammation and repair. Chronic obstructive pulmonary disease (COPD) is characterized by defective lung repair. Yet, BM composition has not been previously characterized in COPD patient...
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doaj-5ac85ef29fd94620b68dbea3702116832020-11-25T02:12:18ZengBMCRespiratory Research1465-993X2018-06-011911910.1186/s12931-018-0824-xBone marrow characterization in COPD: a multi-level network analysisNuria Toledo-Pons0Guillaume Noell1Andreas Jahn2Amanda Iglesias3Maria Antonia Duran4Julio Iglesias5Angel Rios6Sergio Scrimini7Rosa Faner8Orlando Gigirey9Alvar Agustí10Borja G. Cosío11CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIICIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIIDepartment of Respiratory Medicine, Hospital Universitari Son Espases-IdISBaCIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIIDepartment of Hematology, Hospital Universitari Son Espases-IdISBaDepartment of Immunology, Hospital Universitari Son Espases-IdISBaDepartment of Respiratory Medicine, Hospital Universitari Son Espases-IdISBaDepartment of Respiratory Medicine, Hospital Universitari Son Espases-IdISBaCIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIIDepartment of Thoracic Surgery, Hospital Universitari Son Espases-IdISBaCIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIICIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos IIIAbstract Background Bone marrow (BM) produces hematopoietic and progenitor cells that contribute to distant organ inflammation and repair. Chronic obstructive pulmonary disease (COPD) is characterized by defective lung repair. Yet, BM composition has not been previously characterized in COPD patients. Methods In this prospective and controlled study, BM was obtained by sternum fine-needle aspiration in 35 COPD patients and 25 healthy controls (10 smokers and 15 never-smokers). BM cell count and immunophenotype were determined by microscopy and flow cytometry, respectively. Circulating inflammatory (C-reactive protein, IL-6, IL-8) and repair markers (HGF, IGF, TGF-β, VEGF) were quantified by ELISA. Results were integrated by multi-level network correlation analysis. Results We found that: (1) there were no major significant pair wise differences between COPD patients and controls in the BM structural characteristics; (2) multi-level network analysis including patients and controls identifies a relation between immunity, repair and lung function not previously described, that remains in the COPD network but is absent in controls; and (3) this novel network identifies eosinophils as a potential mediator relating immunity and repair, particularly in patients with emphysema. Conclusions Overall, these results suggest that BM is activated in COPD with impaired repair capacity in patients with more emphysema and/or higher circulating eosinophils.http://link.springer.com/article/10.1186/s12931-018-0824-xCOPDBone marrowEosinophilNetwork |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nuria Toledo-Pons Guillaume Noell Andreas Jahn Amanda Iglesias Maria Antonia Duran Julio Iglesias Angel Rios Sergio Scrimini Rosa Faner Orlando Gigirey Alvar Agustí Borja G. Cosío |
spellingShingle |
Nuria Toledo-Pons Guillaume Noell Andreas Jahn Amanda Iglesias Maria Antonia Duran Julio Iglesias Angel Rios Sergio Scrimini Rosa Faner Orlando Gigirey Alvar Agustí Borja G. Cosío Bone marrow characterization in COPD: a multi-level network analysis Respiratory Research COPD Bone marrow Eosinophil Network |
author_facet |
Nuria Toledo-Pons Guillaume Noell Andreas Jahn Amanda Iglesias Maria Antonia Duran Julio Iglesias Angel Rios Sergio Scrimini Rosa Faner Orlando Gigirey Alvar Agustí Borja G. Cosío |
author_sort |
Nuria Toledo-Pons |
title |
Bone marrow characterization in COPD: a multi-level network analysis |
title_short |
Bone marrow characterization in COPD: a multi-level network analysis |
title_full |
Bone marrow characterization in COPD: a multi-level network analysis |
title_fullStr |
Bone marrow characterization in COPD: a multi-level network analysis |
title_full_unstemmed |
Bone marrow characterization in COPD: a multi-level network analysis |
title_sort |
bone marrow characterization in copd: a multi-level network analysis |
publisher |
BMC |
series |
Respiratory Research |
issn |
1465-993X |
publishDate |
2018-06-01 |
description |
Abstract Background Bone marrow (BM) produces hematopoietic and progenitor cells that contribute to distant organ inflammation and repair. Chronic obstructive pulmonary disease (COPD) is characterized by defective lung repair. Yet, BM composition has not been previously characterized in COPD patients. Methods In this prospective and controlled study, BM was obtained by sternum fine-needle aspiration in 35 COPD patients and 25 healthy controls (10 smokers and 15 never-smokers). BM cell count and immunophenotype were determined by microscopy and flow cytometry, respectively. Circulating inflammatory (C-reactive protein, IL-6, IL-8) and repair markers (HGF, IGF, TGF-β, VEGF) were quantified by ELISA. Results were integrated by multi-level network correlation analysis. Results We found that: (1) there were no major significant pair wise differences between COPD patients and controls in the BM structural characteristics; (2) multi-level network analysis including patients and controls identifies a relation between immunity, repair and lung function not previously described, that remains in the COPD network but is absent in controls; and (3) this novel network identifies eosinophils as a potential mediator relating immunity and repair, particularly in patients with emphysema. Conclusions Overall, these results suggest that BM is activated in COPD with impaired repair capacity in patients with more emphysema and/or higher circulating eosinophils. |
topic |
COPD Bone marrow Eosinophil Network |
url |
http://link.springer.com/article/10.1186/s12931-018-0824-x |
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