Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance
Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT2...
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Format: | Article |
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Frontiers Media S.A.
2021-09-01
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Series: | Frontiers in Plant Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.745290/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fan Yang Fan Yang Fan Yang Qier Liu Qier Liu Qin Wang Qin Wang Ning Yang Jun Li Jun Li Honshen Wan Honshen Wan Zehou Liu Zehou Liu Sujie Yang Ying Wang Jie Zhang Jie Zhang Hang Liu Xing Fan Wujun Ma Wuyun Yang Wuyun Yang Yonghong Zhou |
spellingShingle |
Fan Yang Fan Yang Fan Yang Qier Liu Qier Liu Qin Wang Qin Wang Ning Yang Jun Li Jun Li Honshen Wan Honshen Wan Zehou Liu Zehou Liu Sujie Yang Ying Wang Jie Zhang Jie Zhang Hang Liu Xing Fan Wujun Ma Wuyun Yang Wuyun Yang Yonghong Zhou Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance Frontiers in Plant Science durum wheat Aegilops tauschii substitution pentaploidy supernumerary spikelets stripe rust |
author_facet |
Fan Yang Fan Yang Fan Yang Qier Liu Qier Liu Qin Wang Qin Wang Ning Yang Jun Li Jun Li Honshen Wan Honshen Wan Zehou Liu Zehou Liu Sujie Yang Ying Wang Jie Zhang Jie Zhang Hang Liu Xing Fan Wujun Ma Wuyun Yang Wuyun Yang Yonghong Zhou |
author_sort |
Fan Yang |
title |
Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance |
title_short |
Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance |
title_full |
Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance |
title_fullStr |
Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance |
title_full_unstemmed |
Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance |
title_sort |
characterization of the durum wheat-aegilops tauschii 4d(4b) disomic substitution line yl-443 with superior characteristics of high yielding and stripe rust resistance |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-09-01 |
description |
Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F7 progeny. Somatic analysis using multicolor fluorescence in situ hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of Aegilops tauschii AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene Yr28 carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The Yr28-specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding. |
topic |
durum wheat Aegilops tauschii substitution pentaploidy supernumerary spikelets stripe rust |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.745290/full |
work_keys_str_mv |
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doaj-5ad2d642947a4b828efa3133da2c51922021-09-30T06:58:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.745290745290Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust ResistanceFan Yang0Fan Yang1Fan Yang2Qier Liu3Qier Liu4Qin Wang5Qin Wang6Ning Yang7Jun Li8Jun Li9Honshen Wan10Honshen Wan11Zehou Liu12Zehou Liu13Sujie Yang14Ying Wang15Jie Zhang16Jie Zhang17Hang Liu18Xing Fan19Wujun Ma20Wuyun Yang21Wuyun Yang22Yonghong Zhou23Triticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaAustralia-China Joint Centre for Wheat Improvement, Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, AustraliaAustralia-China Joint Centre for Wheat Improvement, Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, AustraliaInstitute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaInstitute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaInstitute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaAustralia-China Joint Centre for Wheat Improvement, Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, AustraliaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaAustralia-China Joint Centre for Wheat Improvement, Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, AustraliaCrop Research Institute, Sichuan Academy of Agricultural Sciences (SAAS), Chengdu, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaDurum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F7 progeny. Somatic analysis using multicolor fluorescence in situ hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of Aegilops tauschii AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene Yr28 carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The Yr28-specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding.https://www.frontiersin.org/articles/10.3389/fpls.2021.745290/fulldurum wheatAegilops tauschiisubstitutionpentaploidysupernumerary spikeletsstripe rust |