In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of...
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doaj-5aed9329d3094a1b96c8966b4a47779d2020-11-25T00:03:31ZengMDPI AGPharmaceutics1999-49232019-02-011126110.3390/pharmaceutics11020061pharmaceutics11020061In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with PaclitaxelValentina Coccè0Silvia Franzè1Anna Teresa Brini2Aldo Bruno Giannì3Luisa Pascucci4Emilio Ciusani5Giulio Alessandri6Giampietro Farronato7Loredana Cavicchini8Valeria Sordi9Rita Paroni10Michele Dei Cas11Francesco Cilurzo12Augusto Pessina13CRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyDepartment of Pharmaceutical Science, University of Milan, 20133 Milan, ItalyCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyDepartment of Veterinary Medicine, University of Perugia, 06123 Perugia, ItalyLaboratory of Clinical Pathology and Medical Genetics, Fondazione IRCCS Istituto Neurologico “C. Besta”, 20133 Milan, ItalyCellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, IRCCS Neurological Institute C. Besta, 20133 Milan, ItalyCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyDiabetes Research Institute, IRCCS San Raffaele Scientific Institute, 20132 Milan, ItalyDepartment of Health Sciences of the University of Milan, 20142 Milan, ItalyDepartment of Health Sciences of the University of Milan, 20142 Milan, ItalyDepartment of Pharmaceutical Science, University of Milan, 20133 Milan, ItalyCRC StaMeTec, Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20133 Milan, ItalyInterdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of GinPaMSCs did not affect the growth of cancer cell lines tested in vitro, whereas the secretome of GinPaMSCs primed with paclitaxel (GinPaMSCs/PTX) exerted a significant anticancer effect. GinPaMSCs were able to uptake and then release paclitaxel in amounts pharmacologically effective against cancer cells, as demonstrated in vitro by the direct activity of GinPaMSCs/PTX and their secretome against both human pancreatic carcinoma and squamous carcinoma cells. PTX was associated with extracellular vesicles (EVs) secreted by cells (EVs/PTX), suggesting that PTX is incorporated into exosomes during their biogenesis. The isolation of mesenchymal stromal cells (MSCs) from gingiva is less invasive than that from other tissues (such as bone marrow and fat), and GinPaMSCs provide an optimal substrate for drug-priming to obtain EVs/PTX having anticancer activity. This research may contribute to develop new strategies of cell-mediated drug delivery by EVs that are easy to store without losing function, and could have a superior safety profile in therapy.https://www.mdpi.com/1999-4923/11/2/61gingiva mesenchymal stromal cellspaclitaxelsquamous cell carcinomadrug deliveryexosomes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valentina Coccè Silvia Franzè Anna Teresa Brini Aldo Bruno Giannì Luisa Pascucci Emilio Ciusani Giulio Alessandri Giampietro Farronato Loredana Cavicchini Valeria Sordi Rita Paroni Michele Dei Cas Francesco Cilurzo Augusto Pessina |
spellingShingle |
Valentina Coccè Silvia Franzè Anna Teresa Brini Aldo Bruno Giannì Luisa Pascucci Emilio Ciusani Giulio Alessandri Giampietro Farronato Loredana Cavicchini Valeria Sordi Rita Paroni Michele Dei Cas Francesco Cilurzo Augusto Pessina In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel Pharmaceutics gingiva mesenchymal stromal cells paclitaxel squamous cell carcinoma drug delivery exosomes |
author_facet |
Valentina Coccè Silvia Franzè Anna Teresa Brini Aldo Bruno Giannì Luisa Pascucci Emilio Ciusani Giulio Alessandri Giampietro Farronato Loredana Cavicchini Valeria Sordi Rita Paroni Michele Dei Cas Francesco Cilurzo Augusto Pessina |
author_sort |
Valentina Coccè |
title |
In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel |
title_short |
In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel |
title_full |
In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel |
title_fullStr |
In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel |
title_full_unstemmed |
In Vitro Anticancer Activity of Extracellular Vesicles (EVs) Secreted by Gingival Mesenchymal Stromal Cells Primed with Paclitaxel |
title_sort |
in vitro anticancer activity of extracellular vesicles (evs) secreted by gingival mesenchymal stromal cells primed with paclitaxel |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2019-02-01 |
description |
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which are easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, and characterized by studying their secretome before and after priming with paclitaxel (PTX). The secretome of GinPaMSCs did not affect the growth of cancer cell lines tested in vitro, whereas the secretome of GinPaMSCs primed with paclitaxel (GinPaMSCs/PTX) exerted a significant anticancer effect. GinPaMSCs were able to uptake and then release paclitaxel in amounts pharmacologically effective against cancer cells, as demonstrated in vitro by the direct activity of GinPaMSCs/PTX and their secretome against both human pancreatic carcinoma and squamous carcinoma cells. PTX was associated with extracellular vesicles (EVs) secreted by cells (EVs/PTX), suggesting that PTX is incorporated into exosomes during their biogenesis. The isolation of mesenchymal stromal cells (MSCs) from gingiva is less invasive than that from other tissues (such as bone marrow and fat), and GinPaMSCs provide an optimal substrate for drug-priming to obtain EVs/PTX having anticancer activity. This research may contribute to develop new strategies of cell-mediated drug delivery by EVs that are easy to store without losing function, and could have a superior safety profile in therapy. |
topic |
gingiva mesenchymal stromal cells paclitaxel squamous cell carcinoma drug delivery exosomes |
url |
https://www.mdpi.com/1999-4923/11/2/61 |
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