Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil
The purpose of this study was to clarify the effects of biochar on the diversity of bacteria and fungi in the rice root zone and to reveal the changes in soil microbial community structure in the root zone after biochar application to provide a scientific basis for the improvement of albic soil. Ric...
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doaj-5af1d4b5dcc640ccb248c208ce9fb7102021-08-26T14:07:06ZengMDPI AGMolecules1420-30492021-08-01264783478310.3390/molecules26164783Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic SoilDawei Yin0Hongyu Li1Haize Wang2Xiaohong Guo3Zhihui Wang4Yandong Lv5Guohua Ding6Liang Jin7Yu Lan8College of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agricultural Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Cultivation and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, ChinaCollege of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCollege of Agricultural Science, Shenyang Agricultural University, Shenyang 110161, ChinaThe purpose of this study was to clarify the effects of biochar on the diversity of bacteria and fungi in the rice root zone and to reveal the changes in soil microbial community structure in the root zone after biochar application to provide a scientific basis for the improvement of albic soil. Rice and corn stalk biochar were mixed with albic soil in a pot experiment. Soil samples were collected at the rice maturity stage, soil nutrients were determined, and genomic DNA was extracted. The library was established using polymerase chain reaction (PCR) amplification. The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics. The results revealed that the biochar increased the soil nutrient content of albic soil. The bacteria ACE indexes of treatments of rice straw biochar (SD) and corn straw biochar (SY) were increased by 3.10% and 2.06%, respectively, and the fungi ACE and Chao indices of SD were increased by 7.86% and 14.16%, respectively, compared to conventional control treatment with no biochar (SBCK). The numbers of bacterial and fungal operational taxonomic units (OUT) in SD and SY were increased, respectively, compared to that of SBCK. The relationship between soil bacteria and fungi in the biochar-treated groups was stronger than that in the SBCK. The bacterial and fungal populations were correlated with soil nutrients, which suggested that the impacts of biochar on the soil bacteria and fungi community were indirectly driven by alternation of soil nutrient characteristics. The addition of two types of biochar altered the soil microbial community structure and the effect of rice straw biochar treatment on SD was more pronounced. This study aimed to provide a reference and basic understanding for albic soil improvement by biochar, with good application prospects.https://www.mdpi.com/1420-3049/26/16/4783biocharalbic soilbacteriafungicommunity structure |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dawei Yin Hongyu Li Haize Wang Xiaohong Guo Zhihui Wang Yandong Lv Guohua Ding Liang Jin Yu Lan |
spellingShingle |
Dawei Yin Hongyu Li Haize Wang Xiaohong Guo Zhihui Wang Yandong Lv Guohua Ding Liang Jin Yu Lan Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil Molecules biochar albic soil bacteria fungi community structure |
author_facet |
Dawei Yin Hongyu Li Haize Wang Xiaohong Guo Zhihui Wang Yandong Lv Guohua Ding Liang Jin Yu Lan |
author_sort |
Dawei Yin |
title |
Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil |
title_short |
Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil |
title_full |
Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil |
title_fullStr |
Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil |
title_full_unstemmed |
Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil |
title_sort |
impact of different biochars on microbial community structure in the rhizospheric soil of rice grown in albic soil |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-08-01 |
description |
The purpose of this study was to clarify the effects of biochar on the diversity of bacteria and fungi in the rice root zone and to reveal the changes in soil microbial community structure in the root zone after biochar application to provide a scientific basis for the improvement of albic soil. Rice and corn stalk biochar were mixed with albic soil in a pot experiment. Soil samples were collected at the rice maturity stage, soil nutrients were determined, and genomic DNA was extracted. The library was established using polymerase chain reaction (PCR) amplification. The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics. The results revealed that the biochar increased the soil nutrient content of albic soil. The bacteria ACE indexes of treatments of rice straw biochar (SD) and corn straw biochar (SY) were increased by 3.10% and 2.06%, respectively, and the fungi ACE and Chao indices of SD were increased by 7.86% and 14.16%, respectively, compared to conventional control treatment with no biochar (SBCK). The numbers of bacterial and fungal operational taxonomic units (OUT) in SD and SY were increased, respectively, compared to that of SBCK. The relationship between soil bacteria and fungi in the biochar-treated groups was stronger than that in the SBCK. The bacterial and fungal populations were correlated with soil nutrients, which suggested that the impacts of biochar on the soil bacteria and fungi community were indirectly driven by alternation of soil nutrient characteristics. The addition of two types of biochar altered the soil microbial community structure and the effect of rice straw biochar treatment on SD was more pronounced. This study aimed to provide a reference and basic understanding for albic soil improvement by biochar, with good application prospects. |
topic |
biochar albic soil bacteria fungi community structure |
url |
https://www.mdpi.com/1420-3049/26/16/4783 |
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