Insulin-Like Growth Factor I Receptor (IGF-IR) Ligands and BMI in Squamous Intra-Epithelial Lesion (SIL) of Cervix
Introduction: Cancer cervix is the most common cancer in women in developing countries like India. Several studies have linked insulin-like growth factors-I & II (IGF-I and IGF-II) and IGF binding proteins-3 (IGFBP-3) with pathogenesis of Squamous Intraepithelial Lesion of cervix (SIL). To t...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2016-02-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/7234/17113_CE[Ra1]_F(AK)_PF1(BMAK)_PFA(AK)_PFA2(AK)_PF2(PAG).pdf |
Summary: | Introduction: Cancer cervix is the most common cancer in
women in developing countries like India. Several studies
have linked insulin-like growth factors-I & II (IGF-I and IGF-II)
and IGF binding proteins-3 (IGFBP-3) with pathogenesis of
Squamous Intraepithelial Lesion of cervix (SIL). To the best of
our knowledge, no study has shown any correlation between
circulating C-Peptide levels and SIL.
Aim: The present study has attempted to evaluate the correlation
between SIL and IGF-IR ligands (IGF-I, IGF-II, C-Peptide), IGF
binding protein (IGFBP-3) and Body Mass Index (BMI).
Materials and Methods: The present case-control study
consisted of 31 histologically proven SIL cases and 31 age
matched controls without evidence of SIL. A 10 ml blood
sample was collected in heparinized vial. Plasma was separated
immediately using centrifugation and was stored at -800
C
till further analysis. Plasma levels of IGF-I, IGF-II, C-peptide
and IGFBP3 were measured using commercially available
Enzyme Linked Immunosorbent Assay (ELISA) kit. Height and
weight was noted for calculation of BMI. Bio-effective molar
ratio (BEMR) was calculated as 3.72 x {(0.25 x IGF-I) + (0.032
x IGF-II) + (0.0025 x C-peptide)} / {(1435 + IGFBP-3) – (2.79
x IGF-I) – (2.87 x IGF-II)}. Statistical analysis was performed
using SPSS and Microsoft Excel software employing student
t-test, Mann-Whitney and Chi-square test for trend while binary
logistic regression was used to estimate the odds ratios (OR)
and corresponding 95% Confidence Intervals (CI).
Result: IGF-I, IGF-II levels and BEMR were significantly
increased in SIL compared to controls (p= 0.001, p <0.001, and
p <0.001, respectively). C-Peptide levels were higher in controls
than SIL (p = 0.04). IGFBP-3 & BMI in SIL were not significantly
related when compared with controls. Risk of SIL in 4th quartile
for BEMR, IGF-I, and IGF-II was 12.18(95% CI= 3.13-47.39),
3.94(95% CI = 1.24-12.56), and 4.57(95% CI = 1.42-14.7),
respectively.
Conclusion: Elevated levels of IGF-I and IGF-II are associated
with risk of SIL while BEMR emerges out to be a derived factor
strongly associated with risk of SIL. |
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ISSN: | 2249-782X 0973-709X |