Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome
Forkhead box P3 (Foxp3) gene is an important means in the Treg cells function, in both maintenances of immune tolerance and regulation of response. Epigenetic modifications of the foxp3 gene at its regulatory regions control the chromatin accessibility for the transcription factors and other transc...
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Tehran University of Medical Sciences
2019-12-01
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doaj-5b01d560cb484e60af1782eb9c2cf2982020-11-25T04:11:51ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492019-12-0119110.18502/ijaai.v19i1.2409Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s SyndromeJafar Farhadi0Mohammad Nouri1Alireza Khabbazi2Nasser Samadi3Zohreh Babaloo4Mahdi Azad5Somayeh Abolhasani6Shahriar Alipour7Golamreza Jadideslam8Sam Seydi Shirvani9Ebrahim Sakhinia10Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran AND Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranConnective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranCancer Biochemistry, Cancer Biotechnology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IranDepartment of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IranMolecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran AND Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran AND Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Forkhead box P3 (Foxp3) gene is an important means in the Treg cells function, in both maintenances of immune tolerance and regulation of response. Epigenetic modifications of the foxp3 gene at its regulatory regions control the chromatin accessibility for the transcription factors and other transcriptional regulators in order to control Foxp3 expression. In addition, the methylation status of CpG islands within the Foxp3 promoter and regulatory elements regulate the expression of Foxp3. This study was performed to assess the role of the foxp3 gene in patients with Behçet’s syndrome (BS). Venous blood samples were collected from all participants and peripheral blood mononuclear cells (PBMC) were extracted through Ficoll-Hypaque method. Genomic DNA was randomly sheared by sonication and immunoprecipitated with a monoclonal antibody. The status methylation of the foxp3 gene was estimated in 108 blood samples of active BS patients and healthy individuals (controls); using methylation DNA immunoprecipitation (MeDIP) technique. Expression analysis was carried out; using Real-time PCR. The expression of foxp3 gene in the patients' group (mean±SD: 1.79±1.12) was significantly lower than the healthy group (mean±SD: 2.73±1.33) (p<001). Also, the methylation levels of Foxp3 promoter showed that its level in patients (mean±SD: 2.3±1.16) was higher than the healthy group (mean±SD: 1.85±0.59). However, this increase was not statistically significant (p>0.05). Also, these results indicated that increasing the amount of methylation of the foxp3 gene by reducing its expression leads to an increase and intensifying of the disease. The decrease in Foxp3 expression is possibly associated with hypermethylation of the gene, and it can be considered as a risk factor for BS. Future studies may be needed to identify the capability of specific DNA methylation alterations in this syndrome. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2248Behçet’s syndromeDNA methylationFoxp3DIP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jafar Farhadi Mohammad Nouri Alireza Khabbazi Nasser Samadi Zohreh Babaloo Mahdi Azad Somayeh Abolhasani Shahriar Alipour Golamreza Jadideslam Sam Seydi Shirvani Ebrahim Sakhinia |
spellingShingle |
Jafar Farhadi Mohammad Nouri Alireza Khabbazi Nasser Samadi Zohreh Babaloo Mahdi Azad Somayeh Abolhasani Shahriar Alipour Golamreza Jadideslam Sam Seydi Shirvani Ebrahim Sakhinia Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome Iranian Journal of Allergy, Asthma and Immunology Behçet’s syndrome DNA methylation Foxp3 DIP |
author_facet |
Jafar Farhadi Mohammad Nouri Alireza Khabbazi Nasser Samadi Zohreh Babaloo Mahdi Azad Somayeh Abolhasani Shahriar Alipour Golamreza Jadideslam Sam Seydi Shirvani Ebrahim Sakhinia |
author_sort |
Jafar Farhadi |
title |
Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome |
title_short |
Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome |
title_full |
Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome |
title_fullStr |
Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome |
title_full_unstemmed |
Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Behçet’s Syndrome |
title_sort |
analysis of methylation and expression profile of foxp3 gene in patients with behçet’s syndrome |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2019-12-01 |
description |
Forkhead box P3 (Foxp3) gene is an important means in the Treg cells function, in both maintenances of immune tolerance and regulation of response. Epigenetic modifications of the foxp3 gene at its regulatory regions control the chromatin accessibility for the transcription factors and other transcriptional regulators in order to control Foxp3 expression. In addition, the methylation status of CpG islands within the Foxp3 promoter and regulatory elements regulate the expression of Foxp3. This study was performed to assess the role of the foxp3 gene in patients with Behçet’s syndrome (BS). Venous blood samples were collected from all participants and peripheral blood mononuclear cells (PBMC) were extracted through Ficoll-Hypaque method. Genomic DNA was randomly sheared by sonication and immunoprecipitated with a monoclonal antibody. The status methylation of the foxp3 gene was estimated in 108 blood samples of active BS patients and healthy individuals (controls); using methylation DNA immunoprecipitation (MeDIP) technique. Expression analysis was carried out; using Real-time PCR. The expression of foxp3 gene in the patients' group (mean±SD: 1.79±1.12) was significantly lower than the healthy group (mean±SD: 2.73±1.33) (p<001). Also, the methylation levels of Foxp3 promoter showed that its level in patients (mean±SD: 2.3±1.16) was higher than the healthy group (mean±SD: 1.85±0.59). However, this increase was not statistically significant (p>0.05). Also, these results indicated that increasing the amount of methylation of the foxp3 gene by reducing its expression leads to an increase and intensifying of the disease. The decrease in Foxp3 expression is possibly associated with hypermethylation of the gene, and it can be considered as a risk factor for BS. Future studies may be needed to identify the capability of specific DNA methylation alterations in this syndrome.
|
topic |
Behçet’s syndrome DNA methylation Foxp3 DIP |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2248 |
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