Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner
Consumption of broccoli mediates numerous chemo-protective benefits through the intake of phytochemicals, some of which modulate aryl hydrocarbon receptor (AHR) activity. Whether AHR activation is a critical aspect of the therapeutic potential of dietary broccoli is not known. Here we administered i...
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Elsevier
2017-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617305029 |
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doaj-5b09603c89ec4c358de11f9e9245da1e2021-04-30T07:11:36ZengElsevierJournal of Functional Foods1756-46462017-10-0137685698Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent mannerTroy D. Hubbard0Iain A. Murray1Robert G. Nichols2Kaitlyn Cassel3Michael Podolsky4Guray Kuzu5Yuan Tian6Phillip Smith7Mary J. Kennett8Andrew D. Patterson9Gary H. Perdew10Department of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesCenter for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesThe Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United StatesDepartment of Veterinary and Biomedical Sciences and The Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, United States; Corresponding author at: The Center for Molecular Toxicology and Carcinogenesis, 309 LSB, The Pennsylvania State University, University Park, PA 16802, United States.Consumption of broccoli mediates numerous chemo-protective benefits through the intake of phytochemicals, some of which modulate aryl hydrocarbon receptor (AHR) activity. Whether AHR activation is a critical aspect of the therapeutic potential of dietary broccoli is not known. Here we administered isocaloric diets, with or without supplementation of whole broccoli (15% w/w), to congenic mice expressing the high-affinity Ahrb/b or low-affinity Ahrd/d alleles, for 24 days and examined the effects on AHR activity, intestinal microbial community structure, inflammatory status, and response to chemically induced colitis. Cecal microbial community structure and metabolic potential were segregated according to host dietary and AHR status. Dietary broccoli associated with heightened intestinal AHR activity, decreased microbial abundance of the family Erysipelotrichaceae, and attenuation of colitis. In summary, broccoli consumption elicited an enhanced response in ligand-sensitive Ahrb/b mice, demonstrating that in part the beneficial aspects of dietary broccoli upon intestinal health are associated with heightened AHR activity.http://www.sciencedirect.com/science/article/pii/S1756464617305029AHRICZIndole-3-carbinolBroccoliIntestinal homeostasisAh receptor |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Troy D. Hubbard Iain A. Murray Robert G. Nichols Kaitlyn Cassel Michael Podolsky Guray Kuzu Yuan Tian Phillip Smith Mary J. Kennett Andrew D. Patterson Gary H. Perdew |
| spellingShingle |
Troy D. Hubbard Iain A. Murray Robert G. Nichols Kaitlyn Cassel Michael Podolsky Guray Kuzu Yuan Tian Phillip Smith Mary J. Kennett Andrew D. Patterson Gary H. Perdew Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner Journal of Functional Foods AHR ICZ Indole-3-carbinol Broccoli Intestinal homeostasis Ah receptor |
| author_facet |
Troy D. Hubbard Iain A. Murray Robert G. Nichols Kaitlyn Cassel Michael Podolsky Guray Kuzu Yuan Tian Phillip Smith Mary J. Kennett Andrew D. Patterson Gary H. Perdew |
| author_sort |
Troy D. Hubbard |
| title |
Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner |
| title_short |
Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner |
| title_full |
Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner |
| title_fullStr |
Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner |
| title_full_unstemmed |
Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner |
| title_sort |
dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an ah receptor dependent manner |
| publisher |
Elsevier |
| series |
Journal of Functional Foods |
| issn |
1756-4646 |
| publishDate |
2017-10-01 |
| description |
Consumption of broccoli mediates numerous chemo-protective benefits through the intake of phytochemicals, some of which modulate aryl hydrocarbon receptor (AHR) activity. Whether AHR activation is a critical aspect of the therapeutic potential of dietary broccoli is not known. Here we administered isocaloric diets, with or without supplementation of whole broccoli (15% w/w), to congenic mice expressing the high-affinity Ahrb/b or low-affinity Ahrd/d alleles, for 24 days and examined the effects on AHR activity, intestinal microbial community structure, inflammatory status, and response to chemically induced colitis. Cecal microbial community structure and metabolic potential were segregated according to host dietary and AHR status. Dietary broccoli associated with heightened intestinal AHR activity, decreased microbial abundance of the family Erysipelotrichaceae, and attenuation of colitis. In summary, broccoli consumption elicited an enhanced response in ligand-sensitive Ahrb/b mice, demonstrating that in part the beneficial aspects of dietary broccoli upon intestinal health are associated with heightened AHR activity. |
| topic |
AHR ICZ Indole-3-carbinol Broccoli Intestinal homeostasis Ah receptor |
| url |
http://www.sciencedirect.com/science/article/pii/S1756464617305029 |
| work_keys_str_mv |
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