Dendritic cells transduced with TIPE-2 recombinant adenovirus induces T cells suppression

• Main Authors: Shudong Liu, Jie Wang, Wenyan Li, Hui Shi, Changlong Zhou, Ge Tang, Jiangwei Zhang, Zhao Yang
• Format: Article
• Language: English
• Published: BMC 2021-02-01
• Series: Journal of Inflammation
• Subjects: Dendritic cells; TIPE-2; Adenovirus; T cells suppression
• Online Access: https://doi.org/10.1186/s12950-021-00274-8

Abstract Introduction TIPE-2 has been identified as a negative regulator of both innate and adaptive immunity and is involved in several inflammatory diseases. However, the role of immune suppression of dendritic cells (DCs) transduced with TIPE-2 has not been well studied. Methods In this study, DCs were transduced with TIPE-2 recombinant adenovirus, and then were cocultured with allogeneic CD4+ or CD8 + T cells. The proliferation, cytokine production and activation marker levels of CD4+ or CD8 + T cell were detected. Results The data demonstrated that T cell proliferation, cytokine production and activation marker levels were attenuated after treated with TIPE-2 transduced DCs. Conclusions These results suggested that TIPE-2 transduced DCs are capable of inducing allogeneic CD4+ or CD8 + T cell immune suppression, which provide a promising way for the therapeutical strategies of transplantation or autoimmune diseases.