Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012
Salmonella infection is among the main food-borne gastrointestinal disease. Meat has been recognized as a major source of human illness caused by Salmonella serovars. The presence of Salmonella was detected in 60 samples of fresh beef from retailsof Sanandaj. The presence of Salmonella was assessed...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Islamic Azad University, Tabriz Branch
2012-11-01
|
Series: | Bihdāsht-i Mavādd-i Ghaz̠āyī |
Subjects: | |
Online Access: | http://jfh.iaut.ac.ir/article_517645_bc3cc3cbad75b9f19815e0aaddc0e186.pdf |
id |
doaj-5b34e13eb5c547a5be691eac9eeed9ea |
---|---|
record_format |
Article |
spelling |
doaj-5b34e13eb5c547a5be691eac9eeed9ea2020-11-24T21:24:55ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682012-11-0123 (7) پاییز4148517645Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012H Karimi Darehabi0F Esmailneshad1K Ebrahimi mohammadi2Assistant Professor of Food Hygiene Department, Faculty of Veterinary Science, SanandajBranch, Islamic Azad University, Kurdistan, Iran.Member of young Researchers Club, Islamic Azad University, damegan,IranDepartment of food science and technology, Faculty of agriculture, Islamic Azad University, mahabad branch, Iran.Salmonella infection is among the main food-borne gastrointestinal disease. Meat has been recognized as a major source of human illness caused by Salmonella serovars. The presence of Salmonella was detected in 60 samples of fresh beef from retailsof Sanandaj. The presence of Salmonella was assessed by conventional culture method and confirmed by PCR assay. To confirm the identification of isolated colonies as Salmonella spp. and determining serovars as typhimuriumand enteritidisserovars, a multiplex PCR assay, using three pairs of primers were employed. S141 and S139 for InvAgene, specific for the genus of Salmonella, Fli15 and Tym for FliCgene, specific for typhimurium serovar and Prot6e-5 and Prot6e-6 for Prot6E gene, specific for Enteritidisserovar.12 samples 20% were determined as contaminated with Salmonella sppwith microbial culture method but with PCR method only seven samples 11.66% were confirmed. 4 samples (6.6%) of isolated colonies were confirmed as SalmonellaTyphimuriumand any number of isolated colonies were confirmed as Salmonella Enteritidis , the other isolated colonis were belong to other salmonella serovars. This study showed a relatively highprevalence of salmonella in fresh beef from Sanandaj.http://jfh.iaut.ac.ir/article_517645_bc3cc3cbad75b9f19815e0aaddc0e186.pdfbeefSalmonella typhimuriumSalmonella Enteritidismultiplex PCR |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
H Karimi Darehabi F Esmailneshad K Ebrahimi mohammadi |
spellingShingle |
H Karimi Darehabi F Esmailneshad K Ebrahimi mohammadi Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 Bihdāsht-i Mavādd-i Ghaz̠āyī beef Salmonella typhimurium Salmonella Enteritidis multiplex PCR |
author_facet |
H Karimi Darehabi F Esmailneshad K Ebrahimi mohammadi |
author_sort |
H Karimi Darehabi |
title |
Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 |
title_short |
Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 |
title_full |
Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 |
title_fullStr |
Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 |
title_full_unstemmed |
Contamination of Fresh Beef to Salmonella typhimurium and Salmonella enteritidis in Sanandaj during 2012 |
title_sort |
contamination of fresh beef to salmonella typhimurium and salmonella enteritidis in sanandaj during 2012 |
publisher |
Islamic Azad University, Tabriz Branch |
series |
Bihdāsht-i Mavādd-i Ghaz̠āyī |
issn |
2228-7647 2476-6968 |
publishDate |
2012-11-01 |
description |
Salmonella infection is among the main food-borne gastrointestinal disease. Meat has been recognized as a major source of human illness caused by Salmonella serovars. The presence of Salmonella was detected in 60 samples of fresh beef from retailsof Sanandaj. The presence of Salmonella was assessed by conventional culture method and confirmed by PCR assay. To confirm the identification of isolated colonies as Salmonella spp. and determining serovars as typhimuriumand enteritidisserovars, a multiplex PCR assay, using three pairs of primers were employed. S141 and S139 for InvAgene, specific for the genus of Salmonella, Fli15 and Tym for FliCgene, specific for typhimurium serovar and Prot6e-5 and Prot6e-6 for Prot6E gene, specific for Enteritidisserovar.12 samples 20% were determined as contaminated with Salmonella sppwith microbial culture method but with PCR method only seven samples 11.66% were confirmed. 4 samples (6.6%) of isolated colonies were confirmed as SalmonellaTyphimuriumand any number of isolated colonies were confirmed as Salmonella Enteritidis , the other isolated colonis were belong to other salmonella serovars. This study showed a relatively highprevalence of salmonella in fresh beef from Sanandaj. |
topic |
beef Salmonella typhimurium Salmonella Enteritidis multiplex PCR |
url |
http://jfh.iaut.ac.ir/article_517645_bc3cc3cbad75b9f19815e0aaddc0e186.pdf |
work_keys_str_mv |
AT hkarimidarehabi contaminationoffreshbeeftosalmonellatyphimuriumandsalmonellaenteritidisinsanandajduring2012 AT fesmailneshad contaminationoffreshbeeftosalmonellatyphimuriumandsalmonellaenteritidisinsanandajduring2012 AT kebrahimimohammadi contaminationoffreshbeeftosalmonellatyphimuriumandsalmonellaenteritidisinsanandajduring2012 |
_version_ |
1725986094723366912 |