Morphological and molecular motifs of fibrosing pulmonary injury patterns
Abstract Interstitial lung diseases encompass a large number of entities, which are characterised by a small number of partially overlapping fibrosing injury patterns, either alone or in combination. Thus, the presently applied morphological diagnostic criteria do not reliably discriminate different...
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doaj-5b3519a573004623a8241cb06b11aeed2020-11-25T01:17:19ZengWileyThe Journal of Pathology: Clinical Research2056-45382019-10-015425627110.1002/cjp2.141Morphological and molecular motifs of fibrosing pulmonary injury patternsDanny Jonigk0Helge Stark1Peter Braubach2Lavinia Neubert3Hoen‐oh Shin4Nicole Izykowski5Tobias Welte6Sabina Janciauskiene7Gregor Warnecke8Axel Haverich9Mark Kuehnel10Florian Laenger11Institute of Pathology Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) The German Center for Lung Research (Deutsches Zentrum für Lungenforschung, DZL), Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) The German Center for Lung Research (Deutsches Zentrum für Lungenforschung, DZL), Hannover Medical School (MHH) Hanover GermanyBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) The German Center for Lung Research (Deutsches Zentrum für Lungenforschung, DZL), Hannover Medical School (MHH) Hanover GermanyDepartment of Thoracic Surgery Hannover Medical School (MHH) Hanover GermanyDepartment of Thoracic Surgery Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyInstitute of Pathology Hannover Medical School (MHH) Hanover GermanyAbstract Interstitial lung diseases encompass a large number of entities, which are characterised by a small number of partially overlapping fibrosing injury patterns, either alone or in combination. Thus, the presently applied morphological diagnostic criteria do not reliably discriminate different interstitial lung diseases. We therefore analysed critical regulatory pathways and signalling molecules involved in pulmonary remodelling with regard to their diagnostic suitability. Using laser‐microdissection and microarray techniques, we examined the expression patterns of 45 tissue‐remodelling associated target genes in remodelled and non‐remodelled tissue samples from patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP), non‐specific interstitial pneumonia (NSIP), organising pneumonia (OP) and alveolar fibroelastosis (AFE), as well as controls (81 patients in total). We found a shared usage of pivotal pathways in AFE, NSIP, OP and UIP, but also individual molecular traits, which set the fibrosing injury patterns apart from each other and correlate well with their specific morphological aspects. Comparison of the aberrant gene expression patterns demonstrated that (1) molecular profiling in fibrosing lung diseases is feasible, (2) pulmonary injury patterns can be discriminated with very high confidence on a molecular level (86–100% specificity) using individual gene subsets and (3) these findings can be adapted as suitable diagnostic adjuncts.https://doi.org/10.1002/cjp2.141interstitial lung diseasesidiopathic interstitial pneumoniausual interstitial pneumonianon‐specific interstitial pneumoniaorganising pneumoniaalveolar fibroelastosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Danny Jonigk Helge Stark Peter Braubach Lavinia Neubert Hoen‐oh Shin Nicole Izykowski Tobias Welte Sabina Janciauskiene Gregor Warnecke Axel Haverich Mark Kuehnel Florian Laenger |
spellingShingle |
Danny Jonigk Helge Stark Peter Braubach Lavinia Neubert Hoen‐oh Shin Nicole Izykowski Tobias Welte Sabina Janciauskiene Gregor Warnecke Axel Haverich Mark Kuehnel Florian Laenger Morphological and molecular motifs of fibrosing pulmonary injury patterns The Journal of Pathology: Clinical Research interstitial lung diseases idiopathic interstitial pneumonia usual interstitial pneumonia non‐specific interstitial pneumonia organising pneumonia alveolar fibroelastosis |
author_facet |
Danny Jonigk Helge Stark Peter Braubach Lavinia Neubert Hoen‐oh Shin Nicole Izykowski Tobias Welte Sabina Janciauskiene Gregor Warnecke Axel Haverich Mark Kuehnel Florian Laenger |
author_sort |
Danny Jonigk |
title |
Morphological and molecular motifs of fibrosing pulmonary injury patterns |
title_short |
Morphological and molecular motifs of fibrosing pulmonary injury patterns |
title_full |
Morphological and molecular motifs of fibrosing pulmonary injury patterns |
title_fullStr |
Morphological and molecular motifs of fibrosing pulmonary injury patterns |
title_full_unstemmed |
Morphological and molecular motifs of fibrosing pulmonary injury patterns |
title_sort |
morphological and molecular motifs of fibrosing pulmonary injury patterns |
publisher |
Wiley |
series |
The Journal of Pathology: Clinical Research |
issn |
2056-4538 |
publishDate |
2019-10-01 |
description |
Abstract Interstitial lung diseases encompass a large number of entities, which are characterised by a small number of partially overlapping fibrosing injury patterns, either alone or in combination. Thus, the presently applied morphological diagnostic criteria do not reliably discriminate different interstitial lung diseases. We therefore analysed critical regulatory pathways and signalling molecules involved in pulmonary remodelling with regard to their diagnostic suitability. Using laser‐microdissection and microarray techniques, we examined the expression patterns of 45 tissue‐remodelling associated target genes in remodelled and non‐remodelled tissue samples from patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP), non‐specific interstitial pneumonia (NSIP), organising pneumonia (OP) and alveolar fibroelastosis (AFE), as well as controls (81 patients in total). We found a shared usage of pivotal pathways in AFE, NSIP, OP and UIP, but also individual molecular traits, which set the fibrosing injury patterns apart from each other and correlate well with their specific morphological aspects. Comparison of the aberrant gene expression patterns demonstrated that (1) molecular profiling in fibrosing lung diseases is feasible, (2) pulmonary injury patterns can be discriminated with very high confidence on a molecular level (86–100% specificity) using individual gene subsets and (3) these findings can be adapted as suitable diagnostic adjuncts. |
topic |
interstitial lung diseases idiopathic interstitial pneumonia usual interstitial pneumonia non‐specific interstitial pneumonia organising pneumonia alveolar fibroelastosis |
url |
https://doi.org/10.1002/cjp2.141 |
work_keys_str_mv |
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