The hierarchically organized splitting of chromosomal bands for all human chromosomes
<p>Abstract</p> <p>Background</p> <p>Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.</p> <p>Resu...
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doaj-5b3dd6ad5ad8463c94829ba392c07af02020-11-24T21:14:25ZengBMCMolecular Cytogenetics1755-81662009-01-0121410.1186/1755-8166-2-4The hierarchically organized splitting of chromosomal bands for all human chromosomesLiehr ThomasClaussen UweMrasek KristinWeise AnjaKosyakova NadezdaNelle Heike<p>Abstract</p> <p>Background</p> <p>Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.</p> <p>Results</p> <p>Here we present for the first time the hierarchically organized splitting of chromosomal bands in their sub-bands for all human chromosomes. To do this, array-proved multicolor banding (aMCB) probe-sets for all human chromosomes were applied to normal metaphase spreads of three different G-band levels. We confirmed for all chromosomes to be a general principle that only Giemsa-dark bands split into dark and light sub-bands, as we demonstrated previously by chromosome stretching. Thus, the biological band splitting is in > 50% of the sub-bands different than implemented by the ISCN nomenclature suggesting also a splitting of G-light bands. Locus-specific probes exemplary confirmed the results of MCB.</p> <p>Conclusion</p> <p>Overall, the present study enables a better understanding of chromosome architecture. The observed difference of biological and ISCN band-splitting may be an explanation why mapping data from human genome project do not always fit the cytogenetic mapping.</p> http://www.molecularcytogenetics.org/content/2/1/4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liehr Thomas Claussen Uwe Mrasek Kristin Weise Anja Kosyakova Nadezda Nelle Heike |
spellingShingle |
Liehr Thomas Claussen Uwe Mrasek Kristin Weise Anja Kosyakova Nadezda Nelle Heike The hierarchically organized splitting of chromosomal bands for all human chromosomes Molecular Cytogenetics |
author_facet |
Liehr Thomas Claussen Uwe Mrasek Kristin Weise Anja Kosyakova Nadezda Nelle Heike |
author_sort |
Liehr Thomas |
title |
The hierarchically organized splitting of chromosomal bands for all human chromosomes |
title_short |
The hierarchically organized splitting of chromosomal bands for all human chromosomes |
title_full |
The hierarchically organized splitting of chromosomal bands for all human chromosomes |
title_fullStr |
The hierarchically organized splitting of chromosomal bands for all human chromosomes |
title_full_unstemmed |
The hierarchically organized splitting of chromosomal bands for all human chromosomes |
title_sort |
hierarchically organized splitting of chromosomal bands for all human chromosomes |
publisher |
BMC |
series |
Molecular Cytogenetics |
issn |
1755-8166 |
publishDate |
2009-01-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Chromosome banding is widely used in cytogenetics. However, the biological nature of hierarchically organized splitting of chromosomal bands of human chromosomes is an enigma and has not been, as yet, studied.</p> <p>Results</p> <p>Here we present for the first time the hierarchically organized splitting of chromosomal bands in their sub-bands for all human chromosomes. To do this, array-proved multicolor banding (aMCB) probe-sets for all human chromosomes were applied to normal metaphase spreads of three different G-band levels. We confirmed for all chromosomes to be a general principle that only Giemsa-dark bands split into dark and light sub-bands, as we demonstrated previously by chromosome stretching. Thus, the biological band splitting is in > 50% of the sub-bands different than implemented by the ISCN nomenclature suggesting also a splitting of G-light bands. Locus-specific probes exemplary confirmed the results of MCB.</p> <p>Conclusion</p> <p>Overall, the present study enables a better understanding of chromosome architecture. The observed difference of biological and ISCN band-splitting may be an explanation why mapping data from human genome project do not always fit the cytogenetic mapping.</p> |
url |
http://www.molecularcytogenetics.org/content/2/1/4 |
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