MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9

• Main Authors: Shi C, Yang Y, Zhang L, Yu J, Qin S, Xu H, Gao Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-10-01
• Series: OncoTargets and Therapy
• Subjects: miR-200a-3p; Ovarian cancer; PCDH9
• Online Access: https://www.dovepress.com/mir-200a-3p-promoted-the-malignant-behaviors-of-ovarian-cancer-cells-t-peer-reviewed-article-OTT

Can Shi, Yijun Yang, Lei Zhang, Juanpeng Yu, Shanshan Qin, Hongge Xu, Yingchun Gao Department of Obstetrics and Gynecology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, People’s Republic of ChinaCorrespondence: Yingchun GaoDepartment of Obstetrics and Gynecology, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, #1 Huanghe Road West, Huai’an, Jiangsu 223300, People’s Republic of ChinaEmail [e-mail]: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in the development and progression of ovarian cancer. MiR-200a-3p was found to act as an oncogene in a variety of cancers, however, the expression and function of miR-200a-3p in ovarian cancer has not been characterized.Materials and methods: The expression of miR-200a-3p in ovarian cancer tissues and cell lines was detected by the RT-qPCR. The influence of miR-200a-3p on the growth of ovarian cancer cells was determined with the Cell Counting Kit-8 assay, colony formation and cell invasion assay. The binding of miR-200a-3p with the 3ʹ-untranslated region (UTR) of PDCH9 was detected by luciferase reporter assay. The expression of PCDH9 was investigated by RT-qPCR and Western blot analysis.Results: miR-200a-3p was up-regulated in ovarian cancer tissues and cell lines. Highly expressed miR-200a-3p was significantly associated with the tumor size, tumor metastasis and TNM stage. Overexpression of miR-200a-3p markedly promoted the proliferation, colony formation and invasion of ovarian cancer cells. Functional study uncovered that miR-200a-3p bound the 3ʹ-untranslated region (UTR) of PCDH9 and decreased the expression of PCDH9 in ovarian cancer cells. The expression of miR-200a-3p in ovarian cancer tissues was significantly negatively correlated with that of PCDH9. Restored PCDH9 inhibited the promoting effect of miR-200a-3p on the proliferation of ovarian cancer cells.Conclusion: Our results suggested the potential oncogenic function of miR-200a-3p via modulating PCDH9 in ovarian cancer.Keywords: miR-200a-3p, ovarian cancer, PCDH9