Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs
Multicomponent lipid emulsions are available for critical care of preterm infants. We sought to determine the impact of different lipid emulsions on early priming of the host and its response to an acute stimulus. Pigs delivered 7d preterm (<em>n</em> = 59) were randomized to receive dif...
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Format: | Article |
Language: | English |
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MDPI AG
2021-01-01
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Series: | Nutrients |
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Online Access: | https://www.mdpi.com/2072-6643/13/1/205 |
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doaj-5b75a0bb8a9643db9f7db628c6c44150 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
William Yakah David Ramiro-Cortijo Pratibha Singh Joanne Brown Barbara Stoll Madhulika Kulkarni Berthe C. Oosterloo Doug Burrin Krishna Rao Maddipati Raina N. Fichorova Steven D. Freedman Camilia R. Martin |
spellingShingle |
William Yakah David Ramiro-Cortijo Pratibha Singh Joanne Brown Barbara Stoll Madhulika Kulkarni Berthe C. Oosterloo Doug Burrin Krishna Rao Maddipati Raina N. Fichorova Steven D. Freedman Camilia R. Martin Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs Nutrients fatty acids arachidonic acid lipid metabolism eicosanoids oxylipins metabolomics |
author_facet |
William Yakah David Ramiro-Cortijo Pratibha Singh Joanne Brown Barbara Stoll Madhulika Kulkarni Berthe C. Oosterloo Doug Burrin Krishna Rao Maddipati Raina N. Fichorova Steven D. Freedman Camilia R. Martin |
author_sort |
William Yakah |
title |
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs |
title_short |
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs |
title_full |
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs |
title_fullStr |
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs |
title_full_unstemmed |
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs |
title_sort |
parenteral fish-oil containing lipid emulsions limit initial lipopolysaccharide-induced host immune responses in preterm pigs |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-01-01 |
description |
Multicomponent lipid emulsions are available for critical care of preterm infants. We sought to determine the impact of different lipid emulsions on early priming of the host and its response to an acute stimulus. Pigs delivered 7d preterm (<em>n</em> = 59) were randomized to receive different lipid emulsions for 11 days: 100% soybean oil (SO), mixed oil emulsion (SO, medium chain olive oil and fish oil) including 15% fish oil (MO15), or 100% fish oil (FO100). On day 11, pigs received an 8-h continuous intravenous infusion of either lipopolysaccharide (LPS—lyophilized <em>Escherichia coli</em>) or saline. Plasma was collected for fatty acid, oxylipin, metabolomic, and cytokine analyses. At day 11, plasma omega-3 fatty acid levels in the FO100 groups showed the highest increase in eicosapentaenoic acid, EPA (0.1 ± 0.0 to 9.7 ± 1.9, <em>p </em>< 0.001), docosahexaenoic acid, DHA (day 0 = 2.5 ± 0.7 to 13.6 ± 2.9, <em>p </em>< 0.001), EPA and DHA-derived oxylipins, and sphingomyelin metabolites. In the SO group, levels of cytokine IL1β increased at the first hour of LPS infusion (296.6 ± 308 pg/mL) but was undetectable in MO15, FO100, or in the animals receiving saline instead of LPS. Pigs in the SO group showed a significant increase in arachidonic acid (AA)-derived prostaglandins and thromboxanes in the first hour (<em>p </em>< 0.05). No significant changes in oxylipins were observed with either fish-oil containing group during LPS infusion. Host priming with soybean oil in the early postnatal period preserves a higher AA:DHA ratio and the ability to acutely respond to an external stimulus. In contrast, fish-oil containing lipid emulsions increase DHA, exacerbate a deficit in AA, and limit the initial LPS-induced inflammatory responses in preterm pigs. |
topic |
fatty acids arachidonic acid lipid metabolism eicosanoids oxylipins metabolomics |
url |
https://www.mdpi.com/2072-6643/13/1/205 |
work_keys_str_mv |
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spelling |
doaj-5b75a0bb8a9643db9f7db628c6c441502021-01-13T00:04:30ZengMDPI AGNutrients2072-66432021-01-011320520510.3390/nu13010205Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm PigsWilliam Yakah0David Ramiro-Cortijo1Pratibha Singh2Joanne Brown3Barbara Stoll4Madhulika Kulkarni5Berthe C. Oosterloo6Doug Burrin7Krishna Rao Maddipati8Raina N. Fichorova9Steven D. Freedman10Camilia R. Martin11Department of Neonatology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USADivision of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USADivision of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USADivision of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USAUnited States Department of Agriculture-Agricultural Research Service, Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USASection Neonatology, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USAUnited States Department of Agriculture-Agricultural Research Service, Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USAUnited States Department of Agriculture-Agricultural Research Service, Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USADepartment of Pathology, Lipidomics Core Facility, Wayne State University, 42 W Warren Avenue, Detroit, MI 48202, USALaboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women’s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USADivision of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USADepartment of Neonatology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115, USAMulticomponent lipid emulsions are available for critical care of preterm infants. We sought to determine the impact of different lipid emulsions on early priming of the host and its response to an acute stimulus. Pigs delivered 7d preterm (<em>n</em> = 59) were randomized to receive different lipid emulsions for 11 days: 100% soybean oil (SO), mixed oil emulsion (SO, medium chain olive oil and fish oil) including 15% fish oil (MO15), or 100% fish oil (FO100). On day 11, pigs received an 8-h continuous intravenous infusion of either lipopolysaccharide (LPS—lyophilized <em>Escherichia coli</em>) or saline. Plasma was collected for fatty acid, oxylipin, metabolomic, and cytokine analyses. At day 11, plasma omega-3 fatty acid levels in the FO100 groups showed the highest increase in eicosapentaenoic acid, EPA (0.1 ± 0.0 to 9.7 ± 1.9, <em>p </em>< 0.001), docosahexaenoic acid, DHA (day 0 = 2.5 ± 0.7 to 13.6 ± 2.9, <em>p </em>< 0.001), EPA and DHA-derived oxylipins, and sphingomyelin metabolites. In the SO group, levels of cytokine IL1β increased at the first hour of LPS infusion (296.6 ± 308 pg/mL) but was undetectable in MO15, FO100, or in the animals receiving saline instead of LPS. Pigs in the SO group showed a significant increase in arachidonic acid (AA)-derived prostaglandins and thromboxanes in the first hour (<em>p </em>< 0.05). No significant changes in oxylipins were observed with either fish-oil containing group during LPS infusion. Host priming with soybean oil in the early postnatal period preserves a higher AA:DHA ratio and the ability to acutely respond to an external stimulus. In contrast, fish-oil containing lipid emulsions increase DHA, exacerbate a deficit in AA, and limit the initial LPS-induced inflammatory responses in preterm pigs.https://www.mdpi.com/2072-6643/13/1/205fatty acidsarachidonic acidlipid metabolismeicosanoidsoxylipinsmetabolomics |