Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks
Abstract Background Endosymbiotic bacteria inhabit a variety of arthropods including ticks and may have multiple effects on the host’s survival, reproduction or pathogen acquisition and transmission. Rhipicephalus haemaphysaloides is one of the most widely distributed tick species in China. The symb...
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doaj-5b77312c51204cdea79e1023396068682020-11-24T21:59:13ZengBMCParasites & Vectors1756-33052018-04-011111710.1186/s13071-018-2807-7Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticksLan-Hua Li0Yi Zhang1Dan Zhu2School of Publish Health and Management, Weifang Medical UniversityNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite & Vector Biology, Ministry of HealthNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Key Laboratory of Parasite & Vector Biology, Ministry of HealthAbstract Background Endosymbiotic bacteria inhabit a variety of arthropods including ticks and may have multiple effects on the host’s survival, reproduction or pathogen acquisition and transmission. Rhipicephalus haemaphysaloides is one of the most widely distributed tick species in China. The symbiotic bacteria composition and their impacts to R. haemaphysaloides ticks have not been studied. The present study investigated the composition of microbial community in R. haemaphysaloides ticks and then assessed the effects of endosymbionts on the host’s fecundity by antibiotic treatment experiments. Methods The microbial population of female and male R. haemaphysaloides ticks was analyzed using Illumina Miseq sequencing of 16S rRNA gene. Thirty engorged female ticks were then randomly divided into five groups and injected with ampicillin, ciprofloxacin, kanamycin, tetracycline, or phosphate-buffered solution (PBS), respectively. Effects of antibiotic treatments on maternal oviposition, egg hatching and density of endosymbionts were evaluated. Results Illumina Miseq sequencing showed that Coxiella and Rickettsia were the predominant bacterial genera inhabiting R. haemaphysaloides ticks. Antibiotic treatment experiments found that kanamycin reduced the density of Coxiella-like endosymbiont (Coxiella-LE hereafter) in eggs, ciprofloxacin reduced the density of Rickettsia-like endosymbiont (Rickettsia-LE), and tetracycline had effect on both endosymbionts, while ampicillin affected neither. Meanwhile hatching rates of eggs were observed to decrease greatly in the kanamycin or tetracycline-treated group but maintained in the ampicillin or ciprofloxacin-treated group. Furthermore, the reduced hatching rates were found to be associated with density of Coxiella-LE in eggs. Conclusions The findings indicate that Coxiella-LE is essential for the reproduction of R. haemaphysaloides ticks, and that kanamycin can be used to study the role of Coxiella-LE on ticks.http://link.springer.com/article/10.1186/s13071-018-2807-7Rhipicephalus haemaphysaloidesEndosymbiontCoxiellaRickettsiaFecundity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lan-Hua Li Yi Zhang Dan Zhu |
spellingShingle |
Lan-Hua Li Yi Zhang Dan Zhu Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks Parasites & Vectors Rhipicephalus haemaphysaloides Endosymbiont Coxiella Rickettsia Fecundity |
author_facet |
Lan-Hua Li Yi Zhang Dan Zhu |
author_sort |
Lan-Hua Li |
title |
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks |
title_short |
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks |
title_full |
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks |
title_fullStr |
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks |
title_full_unstemmed |
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks |
title_sort |
effects of antibiotic treatment on the fecundity of rhipicephalus haemaphysaloides ticks |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-04-01 |
description |
Abstract Background Endosymbiotic bacteria inhabit a variety of arthropods including ticks and may have multiple effects on the host’s survival, reproduction or pathogen acquisition and transmission. Rhipicephalus haemaphysaloides is one of the most widely distributed tick species in China. The symbiotic bacteria composition and their impacts to R. haemaphysaloides ticks have not been studied. The present study investigated the composition of microbial community in R. haemaphysaloides ticks and then assessed the effects of endosymbionts on the host’s fecundity by antibiotic treatment experiments. Methods The microbial population of female and male R. haemaphysaloides ticks was analyzed using Illumina Miseq sequencing of 16S rRNA gene. Thirty engorged female ticks were then randomly divided into five groups and injected with ampicillin, ciprofloxacin, kanamycin, tetracycline, or phosphate-buffered solution (PBS), respectively. Effects of antibiotic treatments on maternal oviposition, egg hatching and density of endosymbionts were evaluated. Results Illumina Miseq sequencing showed that Coxiella and Rickettsia were the predominant bacterial genera inhabiting R. haemaphysaloides ticks. Antibiotic treatment experiments found that kanamycin reduced the density of Coxiella-like endosymbiont (Coxiella-LE hereafter) in eggs, ciprofloxacin reduced the density of Rickettsia-like endosymbiont (Rickettsia-LE), and tetracycline had effect on both endosymbionts, while ampicillin affected neither. Meanwhile hatching rates of eggs were observed to decrease greatly in the kanamycin or tetracycline-treated group but maintained in the ampicillin or ciprofloxacin-treated group. Furthermore, the reduced hatching rates were found to be associated with density of Coxiella-LE in eggs. Conclusions The findings indicate that Coxiella-LE is essential for the reproduction of R. haemaphysaloides ticks, and that kanamycin can be used to study the role of Coxiella-LE on ticks. |
topic |
Rhipicephalus haemaphysaloides Endosymbiont Coxiella Rickettsia Fecundity |
url |
http://link.springer.com/article/10.1186/s13071-018-2807-7 |
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