Physiological roles of sigma factor SigD in Corynebacterium glutamicum
Abstract Background Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of...
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doaj-5b7b876dd33142ecae399ea426cb853b2020-11-24T21:08:46ZengBMCBMC Microbiology1471-21802017-07-0117111010.1186/s12866-017-1067-6Physiological roles of sigma factor SigD in Corynebacterium glutamicumHironori Taniguchi0Tobias Busche1Thomas Patschkowski2Karsten Niehaus3Miroslav Pátek4Jörn Kalinowski5Volker F. Wendisch6Genetics of Prokaryotes, Faculty of Biology, Bielefeld UniversityCenter for Biotechnology, Bielefeld UniversityCenter for Biotechnology, Bielefeld UniversityCenter for Biotechnology, Bielefeld UniversityInstitute of Microbiology, Academy of Sciences of the Czech RepublicCenter for Biotechnology, Bielefeld UniversityGenetics of Prokaryotes, Faculty of Biology, Bielefeld UniversityAbstract Background Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. Results In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. Conclusions This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.http://link.springer.com/article/10.1186/s12866-017-1067-6Corynebacterium glutamicumSigma factorSigDMycomembraneTrehalose dicorynomycolate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hironori Taniguchi Tobias Busche Thomas Patschkowski Karsten Niehaus Miroslav Pátek Jörn Kalinowski Volker F. Wendisch |
spellingShingle |
Hironori Taniguchi Tobias Busche Thomas Patschkowski Karsten Niehaus Miroslav Pátek Jörn Kalinowski Volker F. Wendisch Physiological roles of sigma factor SigD in Corynebacterium glutamicum BMC Microbiology Corynebacterium glutamicum Sigma factor SigD Mycomembrane Trehalose dicorynomycolate |
author_facet |
Hironori Taniguchi Tobias Busche Thomas Patschkowski Karsten Niehaus Miroslav Pátek Jörn Kalinowski Volker F. Wendisch |
author_sort |
Hironori Taniguchi |
title |
Physiological roles of sigma factor SigD in Corynebacterium glutamicum |
title_short |
Physiological roles of sigma factor SigD in Corynebacterium glutamicum |
title_full |
Physiological roles of sigma factor SigD in Corynebacterium glutamicum |
title_fullStr |
Physiological roles of sigma factor SigD in Corynebacterium glutamicum |
title_full_unstemmed |
Physiological roles of sigma factor SigD in Corynebacterium glutamicum |
title_sort |
physiological roles of sigma factor sigd in corynebacterium glutamicum |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2017-07-01 |
description |
Abstract Background Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. Results In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. Conclusions This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum. |
topic |
Corynebacterium glutamicum Sigma factor SigD Mycomembrane Trehalose dicorynomycolate |
url |
http://link.springer.com/article/10.1186/s12866-017-1067-6 |
work_keys_str_mv |
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1716759449885999104 |