Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes?
It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchym...
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2007-08-01
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Online Access: | https://doi.org/10.3727/000000007783465154 |
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doaj-5b8d28e7510644fba739dd8d1ff9f60b2020-11-25T03:17:52ZengSAGE PublishingCell Transplantation0963-68971555-38922007-08-011610.3727/000000007783465154Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes?Mustapha Najimi0Dung Ngoc Khuu1Philippe Antoine Lysy2Nawal Jazouli3Jorge Abarca4Christine Sempoux5Etienne Marc Sokal6 Université catholique de Louvain, Laboratory of Pediatric Hepatology & Cell Therapy, B-1200 Brussels, Belgium Université catholique de Louvain, Laboratory of Pediatric Hepatology & Cell Therapy, B-1200 Brussels, Belgium Université catholique de Louvain, Laboratory of Pediatric Hepatology & Cell Therapy, B-1200 Brussels, Belgium Université catholique de Louvain, Laboratory of Pediatric Hepatology & Cell Therapy, B-1200 Brussels, Belgium Université catholique de Louvain, Radiodiagnostic, Cliniques universitaires St-Luc, B-1200, Brussels, Belgium Université catholique de Louvain, Anatomo-pathology, Cliniques universitaires St-Luc, B-1200 Brussels, Belgium Université catholique de Louvain, Laboratory of Pediatric Hepatology & Cell Therapy, B-1200 Brussels, BelgiumIt is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA+/+-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and α-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA+/+-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and α-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases.https://doi.org/10.3727/000000007783465154 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mustapha Najimi Dung Ngoc Khuu Philippe Antoine Lysy Nawal Jazouli Jorge Abarca Christine Sempoux Etienne Marc Sokal |
spellingShingle |
Mustapha Najimi Dung Ngoc Khuu Philippe Antoine Lysy Nawal Jazouli Jorge Abarca Christine Sempoux Etienne Marc Sokal Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? Cell Transplantation |
author_facet |
Mustapha Najimi Dung Ngoc Khuu Philippe Antoine Lysy Nawal Jazouli Jorge Abarca Christine Sempoux Etienne Marc Sokal |
author_sort |
Mustapha Najimi |
title |
Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? |
title_short |
Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? |
title_full |
Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? |
title_fullStr |
Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? |
title_full_unstemmed |
Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes? |
title_sort |
adult-derived human liver mesenchymal-like cells as a potential progenitor reservoir of hepatocytes? |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2007-08-01 |
description |
It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA+/+-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and α-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA+/+-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and α-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases. |
url |
https://doi.org/10.3727/000000007783465154 |
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