Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males
Protein oxidation may play a role in the balance between anabolism and catabolism. We assessed the effect of a protein restricted diet on protein oxidation as a possible reflection of whole body protein metabolism. Sixteen healthy males (23 ± 3 years) were instructed to use a 4-day isocal...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-01-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6643/11/1/115 |
id |
doaj-5b9c6f98fea3443ebffd2c0082c87387 |
---|---|
record_format |
Article |
spelling |
doaj-5b9c6f98fea3443ebffd2c0082c873872020-11-25T00:03:25ZengMDPI AGNutrients2072-66432019-01-0111111510.3390/nu11010115nu11010115Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young MalesGerlof A.R. Reckman0Gerjan J. Navis1Wim P. Krijnen2Cees P. van der Schans3Roel J. Vonk4Harriët Jager-Wittenaar5Department of Internal Medicine, Division of Nephrology, University of Groningen, University Medical Center Groningen, AA53, PO Box 30.001, 9700 RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University of Groningen, University Medical Center Groningen, AA53, PO Box 30.001, 9700 RB Groningen, The NetherlandsResearch Group Healthy Ageing, Allied Health Care and Nursing, Centre of Expertise Healthy Ageing, Hanze University of Applied Sciences, Petrus Driessenstraat 3, 9714 CA Groningen, The NetherlandsResearch Group Healthy Ageing, Allied Health Care and Nursing, Centre of Expertise Healthy Ageing, Hanze University of Applied Sciences, Petrus Driessenstraat 3, 9714 CA Groningen, The NetherlandsDepartment of Cell Biology, University of Groningen, University Medical Center Groningen, FB33, PO Box 30.001, 9700 RB Groningen, The NetherlandsResearch Group Healthy Ageing, Allied Health Care and Nursing, Centre of Expertise Healthy Ageing, Hanze University of Applied Sciences, Petrus Driessenstraat 3, 9714 CA Groningen, The NetherlandsProtein oxidation may play a role in the balance between anabolism and catabolism. We assessed the effect of a protein restricted diet on protein oxidation as a possible reflection of whole body protein metabolism. Sixteen healthy males (23 ± 3 years) were instructed to use a 4-day isocaloric protein restricted diet (0.25 g protein/kg body weight/day). Their habitual dietary intake was assessed by a 4-day food diary. After an overnight fast, a 30 g 13C-milk protein test drink was administered, followed by 330 min breath sample collection. Protein oxidation was measured by Isotope Ratio Mass Spectrometry. To assess actual change in protein intake from 24-h urea excretion, 24-h urine was collected. During the 4-day protein restricted diet, the urinary urea:creatinine ratio decreased by 56 ± 9%, which is comparable to a protein intake of ~0.65 g protein/kg body weight/day. After the protein restricted diet, 30.5 ± 7.3% of the 30 g 13C-milk protein was oxidized over 330 min, compared to 31.5 ± 6.4% (NS) after the subject’s habitual diet (1.3 ± 0.3 g protein/kg body weight/day). A large range in the effect of the diet on protein oxidation (−43.2% vs. +44.0%) was observed. The residual standard deviation of the measurements was very small (0.601 ± 0.167). This suggests that in healthy males, protein oxidation is unaffected after a protein restricted diet. It is uncertain how important the role of fluctuations in short-term protein oxidation is within whole body protein metabolism.http://www.mdpi.com/2072-6643/11/1/115Proteinoxidationanabolic competencebreath testnaturally enriched 13C-milk proteins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gerlof A.R. Reckman Gerjan J. Navis Wim P. Krijnen Cees P. van der Schans Roel J. Vonk Harriët Jager-Wittenaar |
spellingShingle |
Gerlof A.R. Reckman Gerjan J. Navis Wim P. Krijnen Cees P. van der Schans Roel J. Vonk Harriët Jager-Wittenaar Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males Nutrients Protein oxidation anabolic competence breath test naturally enriched 13C-milk proteins |
author_facet |
Gerlof A.R. Reckman Gerjan J. Navis Wim P. Krijnen Cees P. van der Schans Roel J. Vonk Harriët Jager-Wittenaar |
author_sort |
Gerlof A.R. Reckman |
title |
Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males |
title_short |
Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males |
title_full |
Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males |
title_fullStr |
Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males |
title_full_unstemmed |
Whole Body Protein Oxidation Unaffected after a Protein Restricted Diet in Healthy Young Males |
title_sort |
whole body protein oxidation unaffected after a protein restricted diet in healthy young males |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-01-01 |
description |
Protein oxidation may play a role in the balance between anabolism and catabolism. We assessed the effect of a protein restricted diet on protein oxidation as a possible reflection of whole body protein metabolism. Sixteen healthy males (23 ± 3 years) were instructed to use a 4-day isocaloric protein restricted diet (0.25 g protein/kg body weight/day). Their habitual dietary intake was assessed by a 4-day food diary. After an overnight fast, a 30 g 13C-milk protein test drink was administered, followed by 330 min breath sample collection. Protein oxidation was measured by Isotope Ratio Mass Spectrometry. To assess actual change in protein intake from 24-h urea excretion, 24-h urine was collected. During the 4-day protein restricted diet, the urinary urea:creatinine ratio decreased by 56 ± 9%, which is comparable to a protein intake of ~0.65 g protein/kg body weight/day. After the protein restricted diet, 30.5 ± 7.3% of the 30 g 13C-milk protein was oxidized over 330 min, compared to 31.5 ± 6.4% (NS) after the subject’s habitual diet (1.3 ± 0.3 g protein/kg body weight/day). A large range in the effect of the diet on protein oxidation (−43.2% vs. +44.0%) was observed. The residual standard deviation of the measurements was very small (0.601 ± 0.167). This suggests that in healthy males, protein oxidation is unaffected after a protein restricted diet. It is uncertain how important the role of fluctuations in short-term protein oxidation is within whole body protein metabolism. |
topic |
Protein oxidation anabolic competence breath test naturally enriched 13C-milk proteins |
url |
http://www.mdpi.com/2072-6643/11/1/115 |
work_keys_str_mv |
AT gerlofarreckman wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales AT gerjanjnavis wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales AT wimpkrijnen wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales AT ceespvanderschans wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales AT roeljvonk wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales AT harrietjagerwittenaar wholebodyproteinoxidationunaffectedafteraproteinrestricteddietinhealthyyoungmales |
_version_ |
1725434133508784128 |