Trio-Based Deep Sequencing Reveals a Low Incidence of Off-Target Mutations in the Offspring of Genetically Edited Goats

Trio-Based Deep Sequencing Reveals a Low Incidence of Off-Target Mutations in the Offspring of Genetically Edited Goats

Unintended off-target mutations induced by CRISPR/Cas9 nucleases may result in unwanted consequences, which will impede the efficient applicability of this technology for genetic improvement. We have recently edited the goat genome through CRISPR/Cas9 by targeting MSTN and FGF5, which increased musc...

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Bibliographic Details
Main Authors: Chao Li, Shiwei Zhou, Yan Li, Guanwei Li, Yige Ding, Lan Li, Jing Liu, Lei Qu, Tad Sonstegard, Xingxu Huang, Yu Jiang, Yulin Chen, Bjoern Petersen, Xiaolong Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Genetics
Subjects:
genome editing
CRISPR/Cas9
whole genome sequencing
off-target
de novo mutation
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2018.00449/full

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