Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects
Abstract Background Despite the potential usefulness of optical coherence tomography angiography in retinal and optic disc conditions, the reliability of the imaging modality remains unclear. This study set out to measure the microvascular density of macula and optic disc by mean of optical coherenc...
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doaj-5bb3931645e34cc4947c34d28f5604292020-11-24T21:52:48ZengBMCBMC Ophthalmology1471-24152018-12-0118111010.1186/s12886-018-0976-yOptical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjectsChing Wei Lim0Jun Cheng1Elton Lik Tong Tay2Hwei Yee Teo3Elizabeth Poh Ying Wong4Vernon Khet Yau Yong5Boon Ang Lim6Owen Kim Hee7Hon Tym Wong8Leonard Wei Leon Yip9Department of Ophthalmology, Tan Tock Seng HospitalOcular Imaging Department, Institute for Infocomm Research, Agency for Science, Technology and ResearchDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalDepartment of Ophthalmology, Tan Tock Seng HospitalAbstract Background Despite the potential usefulness of optical coherence tomography angiography in retinal and optic disc conditions, the reliability of the imaging modality remains unclear. This study set out to measure the microvascular density of macula and optic disc by mean of optical coherence tomography angiography and report the repeatability of the vessel density measurements. Methods Cross sectional observational cohort study. Subjects with normal eyes were recruited. Two sets of optical coherence tomography angiography images of macula and optic nerve head were acquired during one visit. Novel in-house developed software was used to count the pixels in each images and to compute the microvessel density of the macula and optic disc. Data were analysed to determine the measurement repeatability. Results A total of 176 eyes from 88 consecutive normal subjects were recruited. For macular images, the mean vessel density at superficial retina, deep retina, outer retina and choriocapillaries segment was OD 0.113 and OS 0.111, OD 0.239 and OS 0.230, OD 0.179 and OS 0.164, OD 0.237 and OS 0.215 respectively. For optic disc images, mean vessel density at vitreoretinal interface, radial peripapillary capillary, superficial nerve head and disc segment at the level of choroid were OD 0.084 and OS 0.085, OD 0.140 and OS 0.138, OD 0.216 and OS 0.209, OD 0.227 and OS 0.236 respectively. The measurement repeatability tests showed that the coefficient of variation of macular scans, for right and left eyes, ranged from 6.4 to 31.1% and 5.3 to 59.4%. Likewise, the coefficient of variation of optic disc scans, for right and left eyes, ranged from 14.3 to 77.4% and 13.5 to 75.3%. Conclusions Optical coherence tomography angiography is a useful modality to visualise the microvasculature plexus of macula and optic nerve head. The vessel density measurement of macular scan by mean of optical coherence tomography angiography demonstrated good repeatability. The optic disc scan, on the other hand, showed a higher coefficient of variation indicating a lower measurement repeatability than macular scan. Interpretation of optical coherence tomography angiography should take into account test-retest repeatability of the imaging system. Trial registration National Healthcare Group Domain Specific Review Board (NHG DSRB) Singapore. DSRB Reference: 2015/00301.http://link.springer.com/article/10.1186/s12886-018-0976-yOptical coherence tomography angiographyTest-retest repeatabilityIntra-observer variationHealthy volunteers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ching Wei Lim Jun Cheng Elton Lik Tong Tay Hwei Yee Teo Elizabeth Poh Ying Wong Vernon Khet Yau Yong Boon Ang Lim Owen Kim Hee Hon Tym Wong Leonard Wei Leon Yip |
spellingShingle |
Ching Wei Lim Jun Cheng Elton Lik Tong Tay Hwei Yee Teo Elizabeth Poh Ying Wong Vernon Khet Yau Yong Boon Ang Lim Owen Kim Hee Hon Tym Wong Leonard Wei Leon Yip Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects BMC Ophthalmology Optical coherence tomography angiography Test-retest repeatability Intra-observer variation Healthy volunteers |
author_facet |
Ching Wei Lim Jun Cheng Elton Lik Tong Tay Hwei Yee Teo Elizabeth Poh Ying Wong Vernon Khet Yau Yong Boon Ang Lim Owen Kim Hee Hon Tym Wong Leonard Wei Leon Yip |
author_sort |
Ching Wei Lim |
title |
Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
title_short |
Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
title_full |
Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
title_fullStr |
Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
title_full_unstemmed |
Optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
title_sort |
optical coherence tomography angiography of the macula and optic nerve head: microvascular density and test-retest repeatability in normal subjects |
publisher |
BMC |
series |
BMC Ophthalmology |
issn |
1471-2415 |
publishDate |
2018-12-01 |
description |
Abstract Background Despite the potential usefulness of optical coherence tomography angiography in retinal and optic disc conditions, the reliability of the imaging modality remains unclear. This study set out to measure the microvascular density of macula and optic disc by mean of optical coherence tomography angiography and report the repeatability of the vessel density measurements. Methods Cross sectional observational cohort study. Subjects with normal eyes were recruited. Two sets of optical coherence tomography angiography images of macula and optic nerve head were acquired during one visit. Novel in-house developed software was used to count the pixels in each images and to compute the microvessel density of the macula and optic disc. Data were analysed to determine the measurement repeatability. Results A total of 176 eyes from 88 consecutive normal subjects were recruited. For macular images, the mean vessel density at superficial retina, deep retina, outer retina and choriocapillaries segment was OD 0.113 and OS 0.111, OD 0.239 and OS 0.230, OD 0.179 and OS 0.164, OD 0.237 and OS 0.215 respectively. For optic disc images, mean vessel density at vitreoretinal interface, radial peripapillary capillary, superficial nerve head and disc segment at the level of choroid were OD 0.084 and OS 0.085, OD 0.140 and OS 0.138, OD 0.216 and OS 0.209, OD 0.227 and OS 0.236 respectively. The measurement repeatability tests showed that the coefficient of variation of macular scans, for right and left eyes, ranged from 6.4 to 31.1% and 5.3 to 59.4%. Likewise, the coefficient of variation of optic disc scans, for right and left eyes, ranged from 14.3 to 77.4% and 13.5 to 75.3%. Conclusions Optical coherence tomography angiography is a useful modality to visualise the microvasculature plexus of macula and optic nerve head. The vessel density measurement of macular scan by mean of optical coherence tomography angiography demonstrated good repeatability. The optic disc scan, on the other hand, showed a higher coefficient of variation indicating a lower measurement repeatability than macular scan. Interpretation of optical coherence tomography angiography should take into account test-retest repeatability of the imaging system. Trial registration National Healthcare Group Domain Specific Review Board (NHG DSRB) Singapore. DSRB Reference: 2015/00301. |
topic |
Optical coherence tomography angiography Test-retest repeatability Intra-observer variation Healthy volunteers |
url |
http://link.springer.com/article/10.1186/s12886-018-0976-y |
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