Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon
The emergence of insecticide resistance in <i>Aedes mosquitoes</i> could pose major challenges for arboviral-borne disease control. In this paper, insecticide susceptibility level and resistance mechanisms were assessed in <i>Aedes aegypti</i> (Linnaeus, 1762) and <i>Ae...
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MDPI AG
2021-05-01
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Online Access: | https://www.mdpi.com/2073-4425/12/6/828 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Borel Djiappi-Tchamen Mariette Stella Nana-Ndjangwo Konstantinos Mavridis Abdou Talipouo Elysée Nchoutpouen Idene Makoudjou Roland Bamou Audrey Marie Paul Mayi Parfait Awono-Ambene Timoléon Tchuinkam John Vontas Christophe Antonio-Nkondjio |
spellingShingle |
Borel Djiappi-Tchamen Mariette Stella Nana-Ndjangwo Konstantinos Mavridis Abdou Talipouo Elysée Nchoutpouen Idene Makoudjou Roland Bamou Audrey Marie Paul Mayi Parfait Awono-Ambene Timoléon Tchuinkam John Vontas Christophe Antonio-Nkondjio Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon Genes <i>Aedes aegypti</i> <i>Aedes albopictus</i> insecticide resistance diagnostics arbovirus mechanisms urban settings |
author_facet |
Borel Djiappi-Tchamen Mariette Stella Nana-Ndjangwo Konstantinos Mavridis Abdou Talipouo Elysée Nchoutpouen Idene Makoudjou Roland Bamou Audrey Marie Paul Mayi Parfait Awono-Ambene Timoléon Tchuinkam John Vontas Christophe Antonio-Nkondjio |
author_sort |
Borel Djiappi-Tchamen |
title |
Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon |
title_short |
Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon |
title_full |
Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon |
title_fullStr |
Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon |
title_full_unstemmed |
Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from Cameroon |
title_sort |
analyses of insecticide resistance genes in <i>aedes aegypti</i> and <i>aedes albopictus</i> mosquito populations from cameroon |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2021-05-01 |
description |
The emergence of insecticide resistance in <i>Aedes mosquitoes</i> could pose major challenges for arboviral-borne disease control. In this paper, insecticide susceptibility level and resistance mechanisms were assessed in <i>Aedes aegypti</i> (Linnaeus, 1762) and <i>Aedes albopictus</i> (Skuse, 1894) from urban settings of Cameroon. The F1 progeny of <i>Aedes aegypti</i> and <i>Aedes albopictus</i> collected in Douala, Yaoundé and Dschang from August to December 2020 was tested using WHO tube assays with four insecticides: deltamethrin 0.05%, permethrin 0.75%, DDT 4% and bendiocarb 0.1%. TaqMan, qPCR and RT-qPCR assays were used to detect kdr mutations and the expression profiles of eight detoxification genes. <i>Aedes aegypti</i> mosquitoes from Douala were found to be resistant to DDT, permethrin and deltamethrin. Three kdr mutations, F1534C, V1016G and V1016I were detected in <i>Aedes aegypti</i> populations from Douala and Dschang. The kdr allele F1534C was predominant (90%) in <i>Aedes aegypti</i> and was detected for the first time in <i>Aedes albopictus</i> (2.08%). P450s genes, Cyp9J28 (2.23–7.03 folds), Cyp9M6 (1.49–2.59 folds), Cyp9J32 (1.29–3.75 folds) and GSTD4 (1.34–55.3 folds) were found overexpressed in the Douala and Yaoundé <i>Aedes aegypti</i> populations. The emergence of insecticide resistance in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> calls for alternative strategies towards the control and prevention of arboviral vector-borne diseases in Cameroon. |
topic |
<i>Aedes aegypti</i> <i>Aedes albopictus</i> insecticide resistance diagnostics arbovirus mechanisms urban settings |
url |
https://www.mdpi.com/2073-4425/12/6/828 |
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doaj-5bb6ac15e43d4b2ead3b793169dfb2b92021-06-01T01:25:48ZengMDPI AGGenes2073-44252021-05-011282882810.3390/genes12060828Analyses of Insecticide Resistance Genes in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquito Populations from CameroonBorel Djiappi-Tchamen0Mariette Stella Nana-Ndjangwo1Konstantinos Mavridis2Abdou Talipouo3Elysée Nchoutpouen4Idene Makoudjou5Roland Bamou6Audrey Marie Paul Mayi7Parfait Awono-Ambene8Timoléon Tchuinkam9John Vontas10Christophe Antonio-Nkondjio11Vector Borne Diseases Laboratory of the Applied Biology and Ecology Research Unit (VBID-URBEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067 Dschang, CameroonInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 70013 Heraklion, GreeceInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonVector Borne Diseases Laboratory of the Applied Biology and Ecology Research Unit (VBID-URBEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067 Dschang, CameroonVector Borne Diseases Laboratory of the Applied Biology and Ecology Research Unit (VBID-URBEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067 Dschang, CameroonInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonVector Borne Diseases Laboratory of the Applied Biology and Ecology Research Unit (VBID-URBEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067 Dschang, CameroonInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 70013 Heraklion, GreeceInstitut de Recherche de Yaoundé (IRY), Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288 Yaoundé, CameroonThe emergence of insecticide resistance in <i>Aedes mosquitoes</i> could pose major challenges for arboviral-borne disease control. In this paper, insecticide susceptibility level and resistance mechanisms were assessed in <i>Aedes aegypti</i> (Linnaeus, 1762) and <i>Aedes albopictus</i> (Skuse, 1894) from urban settings of Cameroon. The F1 progeny of <i>Aedes aegypti</i> and <i>Aedes albopictus</i> collected in Douala, Yaoundé and Dschang from August to December 2020 was tested using WHO tube assays with four insecticides: deltamethrin 0.05%, permethrin 0.75%, DDT 4% and bendiocarb 0.1%. TaqMan, qPCR and RT-qPCR assays were used to detect kdr mutations and the expression profiles of eight detoxification genes. <i>Aedes aegypti</i> mosquitoes from Douala were found to be resistant to DDT, permethrin and deltamethrin. Three kdr mutations, F1534C, V1016G and V1016I were detected in <i>Aedes aegypti</i> populations from Douala and Dschang. The kdr allele F1534C was predominant (90%) in <i>Aedes aegypti</i> and was detected for the first time in <i>Aedes albopictus</i> (2.08%). P450s genes, Cyp9J28 (2.23–7.03 folds), Cyp9M6 (1.49–2.59 folds), Cyp9J32 (1.29–3.75 folds) and GSTD4 (1.34–55.3 folds) were found overexpressed in the Douala and Yaoundé <i>Aedes aegypti</i> populations. The emergence of insecticide resistance in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> calls for alternative strategies towards the control and prevention of arboviral vector-borne diseases in Cameroon.https://www.mdpi.com/2073-4425/12/6/828<i>Aedes aegypti</i><i>Aedes albopictus</i>insecticide resistance diagnosticsarbovirusmechanismsurban settings |