Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood
Abstract Microbiota plays an important role in regulating immune responses associated with atopic diseases. We sought to evaluate relationships among airway microbiota, serum IgE levels, allergic sensitization and their relevance to rhinitis and asthma. Microbial characterization was performed using...
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doaj-5bd0682a1b6541eb98b59664722544522020-12-08T02:35:48ZengNature Publishing GroupScientific Reports2045-23222017-05-01711810.1038/s41598-017-02067-7Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early ChildhoodChih-Yung Chiu0Yi-Ling Chan1Yu-Shuen Tsai2Ssu-An Chen3Chia-Jung Wang4Kuan-Fu Chen5I.-Fang Chung6Department of Pediatrics, Chang Gung Memorial Hospital at Keelung, and Chang Gung UniversityDepartment of Emergency Medicine, Chang Gung Memorial Hospital at LinkouThe Institute of Biomedical Informatics, National Yang-Ming UniversityThe Institute of Biomedical Informatics, National Yang-Ming UniversityDepartment of Pediatrics, Chang Gung Memorial Hospital at Keelung, and Chang Gung UniversityDepartment of Emergency Medicine, Chang Gung Memorial Hospital at LinkouThe Institute of Biomedical Informatics, National Yang-Ming UniversityAbstract Microbiota plays an important role in regulating immune responses associated with atopic diseases. We sought to evaluate relationships among airway microbiota, serum IgE levels, allergic sensitization and their relevance to rhinitis and asthma. Microbial characterization was performed using Illumina-based 16S rRNA gene sequencing of 87 throat swabs collected from children with asthma (n = 32) and rhinitis (n = 23), and from healthy controls (n = 32). Data analysis was performed using QIIME (Quantitative Insights Into Microbial Ecology) v1.8. Significantly higher abundance of Proteobacteria was found in children with rhinitis than in the healthy controls (20.1% vs. 16.1%, P = 0.009). Bacterial species richness (Chao1 index) and diversity (Shannon index) were significantly reduced in children with mite sensitization but not in those with food or IgE sensitization. Compared with healthy children without mite sensitization, the mite-sensitized children with rhinitis and asthma showed significantly lower Chao1 and Shannon indices. Moraxella and Leptotrichia species were significantly found in the interaction of mite sensitization with rhinitis and asthma respectively. Airway microbial diversity appears to be inversely associated with sensitization to house dust mites. A modulation between airway dysbiosis and responses to allergens may potentially cause susceptibility to rhinitis and asthma in early childhood.https://doi.org/10.1038/s41598-017-02067-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chih-Yung Chiu Yi-Ling Chan Yu-Shuen Tsai Ssu-An Chen Chia-Jung Wang Kuan-Fu Chen I.-Fang Chung |
spellingShingle |
Chih-Yung Chiu Yi-Ling Chan Yu-Shuen Tsai Ssu-An Chen Chia-Jung Wang Kuan-Fu Chen I.-Fang Chung Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood Scientific Reports |
author_facet |
Chih-Yung Chiu Yi-Ling Chan Yu-Shuen Tsai Ssu-An Chen Chia-Jung Wang Kuan-Fu Chen I.-Fang Chung |
author_sort |
Chih-Yung Chiu |
title |
Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood |
title_short |
Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood |
title_full |
Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood |
title_fullStr |
Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood |
title_full_unstemmed |
Airway Microbial Diversity is Inversely Associated with Mite-Sensitized Rhinitis and Asthma in Early Childhood |
title_sort |
airway microbial diversity is inversely associated with mite-sensitized rhinitis and asthma in early childhood |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-05-01 |
description |
Abstract Microbiota plays an important role in regulating immune responses associated with atopic diseases. We sought to evaluate relationships among airway microbiota, serum IgE levels, allergic sensitization and their relevance to rhinitis and asthma. Microbial characterization was performed using Illumina-based 16S rRNA gene sequencing of 87 throat swabs collected from children with asthma (n = 32) and rhinitis (n = 23), and from healthy controls (n = 32). Data analysis was performed using QIIME (Quantitative Insights Into Microbial Ecology) v1.8. Significantly higher abundance of Proteobacteria was found in children with rhinitis than in the healthy controls (20.1% vs. 16.1%, P = 0.009). Bacterial species richness (Chao1 index) and diversity (Shannon index) were significantly reduced in children with mite sensitization but not in those with food or IgE sensitization. Compared with healthy children without mite sensitization, the mite-sensitized children with rhinitis and asthma showed significantly lower Chao1 and Shannon indices. Moraxella and Leptotrichia species were significantly found in the interaction of mite sensitization with rhinitis and asthma respectively. Airway microbial diversity appears to be inversely associated with sensitization to house dust mites. A modulation between airway dysbiosis and responses to allergens may potentially cause susceptibility to rhinitis and asthma in early childhood. |
url |
https://doi.org/10.1038/s41598-017-02067-7 |
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