In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8
<p>Abstract</p> <p>Background</p> <p>Φ8 is a bacteriophage containing a genome of three segments of double-stranded RNA inside a polyhedral capsid enveloped in a lipid-containing membrane. Plus strand RNA binds and is packaged by empty procapsids. Whereas Φ6, another me...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2005-03-01
|
Series: | BMC Microbiology |
Online Access: | http://www.biomedcentral.com/1471-2180/5/10 |
id |
doaj-5bdfeaa196344a259fd1f07650c763b5 |
---|---|
record_format |
Article |
spelling |
doaj-5bdfeaa196344a259fd1f07650c763b52020-11-25T02:33:52ZengBMCBMC Microbiology1471-21802005-03-01511010.1186/1471-2180-5-10In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8Mindich LeonardQiao XueyingQiao Jian<p>Abstract</p> <p>Background</p> <p>Φ8 is a bacteriophage containing a genome of three segments of double-stranded RNA inside a polyhedral capsid enveloped in a lipid-containing membrane. Plus strand RNA binds and is packaged by empty procapsids. Whereas Φ6, another member of the Cystoviridae, shows high stringency, serial dependence and precision in its genomic packaging <it>in vitro </it>and <it>in vivo</it>, Φ8 packaging is more flexible. Unique sequences (<it>pac</it>) near the 5' ends of plus strands are necessary and sufficient for Φ6 genomic packaging and the RNA binding sites are located on P1, the major structural protein of the procapsid.</p> <p>Results</p> <p>In this paper the boundaries of the Φ8 <it>pac </it>sequences have been explored by testing the <it>in vivo </it>packaging efficacy of transcripts containing deletions or changes in the RNA sequences. The <it>pac </it>sequences have been localized to the 5' untranslated regions of the viral transcripts. Major changes in the <it>pac </it>sequences are either tolerated or ameliorated by suppressor mutations in the RNA sequence. Changes in the genomic packaging program can be established as a result of mutations in P1, the major structural protein of the procapsid and the determinant of RNA binding specificity.</p> <p>Conclusion</p> <p>Although Φ8 is distantly related to bacteriophage Φ6, and does not show sequence similarity, it has a similar genomic packaging program. This program, however, is less stringent than that of Φ6.</p> http://www.biomedcentral.com/1471-2180/5/10 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mindich Leonard Qiao Xueying Qiao Jian |
spellingShingle |
Mindich Leonard Qiao Xueying Qiao Jian In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 BMC Microbiology |
author_facet |
Mindich Leonard Qiao Xueying Qiao Jian |
author_sort |
Mindich Leonard |
title |
In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 |
title_short |
In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 |
title_full |
In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 |
title_fullStr |
In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 |
title_full_unstemmed |
In vivo studies of genomic packaging in the dsRNA bacteriophage Φ8 |
title_sort |
in vivo studies of genomic packaging in the dsrna bacteriophage φ8 |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2005-03-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Φ8 is a bacteriophage containing a genome of three segments of double-stranded RNA inside a polyhedral capsid enveloped in a lipid-containing membrane. Plus strand RNA binds and is packaged by empty procapsids. Whereas Φ6, another member of the Cystoviridae, shows high stringency, serial dependence and precision in its genomic packaging <it>in vitro </it>and <it>in vivo</it>, Φ8 packaging is more flexible. Unique sequences (<it>pac</it>) near the 5' ends of plus strands are necessary and sufficient for Φ6 genomic packaging and the RNA binding sites are located on P1, the major structural protein of the procapsid.</p> <p>Results</p> <p>In this paper the boundaries of the Φ8 <it>pac </it>sequences have been explored by testing the <it>in vivo </it>packaging efficacy of transcripts containing deletions or changes in the RNA sequences. The <it>pac </it>sequences have been localized to the 5' untranslated regions of the viral transcripts. Major changes in the <it>pac </it>sequences are either tolerated or ameliorated by suppressor mutations in the RNA sequence. Changes in the genomic packaging program can be established as a result of mutations in P1, the major structural protein of the procapsid and the determinant of RNA binding specificity.</p> <p>Conclusion</p> <p>Although Φ8 is distantly related to bacteriophage Φ6, and does not show sequence similarity, it has a similar genomic packaging program. This program, however, is less stringent than that of Φ6.</p> |
url |
http://www.biomedcentral.com/1471-2180/5/10 |
work_keys_str_mv |
AT mindichleonard invivostudiesofgenomicpackaginginthedsrnabacteriophageph8 AT qiaoxueying invivostudiesofgenomicpackaginginthedsrnabacteriophageph8 AT qiaojian invivostudiesofgenomicpackaginginthedsrnabacteriophageph8 |
_version_ |
1724811974213107712 |